UDP-sulfoquinovose synthase
| UDPsulfoquinovose synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.13.1.1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / EGO | ||||||||
| |||||||||
UDP-sulfoquinovose synthase (EC 3.13.1.1) is an enzyme that catalyzes the chemical reaction
- UDP-glucose + sulfite
UDP-6-sulfoquinovose + H2O
Thus, the two substrates of this enzyme are UDP-glucose and sulfite, whereas its two products are UDP-6-sulfoquinovose and H2O.
In a subsequent reaction catalyzed by sulfoquinovosyl diacylglycerol synthase, the sulfoquinovose portion of UDP-sulfoquinovose is combined with diacyglycerol to produce the sulfolipid sulfoquinovosyl diacylglycerol (SQDG).
This enzyme belongs to the family of hydrolases, specifically those acting on carbon-sulfur bonds. The systematic name of this enzyme class is UDP-6-sulfo-6-deoxyglucose sulfohydrolase. Other names in common use include sulfite:UDP-glucose sulfotransferase, and UDP-sulfoquinovose synthase. This enzyme participates in nucleotide sugars metabolism and glycerolipid metabolism.
The 3-dimensional structure of the enzyme is known from Protein Data Bank entries 1qrr (Mulichak et al., 1999), 1i24, 1i2b and 1i2c.
See also
References
- Essigmann B, Guler S, Narang RA, Linke D, Benning C (1998). "Phosphate availability affects the thylakoid lipid composition and the expression of SQD1, a gene required for sulfolipid biosynthesis in Arabidopsis thaliana". Proc. Natl. Acad. Sci. U.S.A. 95 (4): 1950–5. doi:10.1073/pnas.95.4.1950. PMC 19220. PMID 9465123.
- Essigmann B, Hespenheide BM, Kuhn LA, Benning C (1999). "Prediction of the active-site structure and NAD(+) binding in SQD1, a protein essential for sulfolipid biosynthesis in Arabidopsis". Arch. Biochem. Biophys. 369 (1): 30–41. doi:10.1006/abbi.1999.1344. PMID 10462438.
- Mulichak AM, Theisen MJ, Essigmann B, Benning C, Garavito RM (1999). "Crystal structure of SQD1, an enzyme involved in the biosynthesis of the plant sulfolipid headgroup donor UDP-sulfoquinovose". Proc. Natl. Acad. Sci. U.S.A. 96 (23): 13097–102. doi:10.1073/pnas.96.23.13097. PMC 23906. PMID 10557279.
- Sanda S, Leustek T, Theisen MJ, Garavito RM, Benning C (2001). "Recombinant Arabidopsis SQD1 converts UDP-glucose and sulfite to the sulfolipid head group precursor UDP-sulfoquinovose in vitro". J. Biol. Chem. 276 (6): 3941–6. doi:10.1074/jbc.M008200200. PMID 11073956.