U2AF2

U2 small nuclear RNA auxiliary factor 2

PDB rendering based on 1jmt.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
SymbolsU2AF2 ; U2AF65
External IDsOMIM: 191318 HomoloGene: 110853 GeneCards: U2AF2 Gene
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez1133822185
EnsemblENSG00000063244ENSMUSG00000030435
UniProtP26368P26369
RefSeq (mRNA)NM_001012478NM_001205231
RefSeq (protein)NP_001012496NP_001192160
Location (UCSC)Chr 19:
56.17 – 56.19 Mb		Chr 7:
5.06 – 5.08 Mb
PubMed search

Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene.[1]

Function

U2 auxiliary factor (U2AF), composed of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit, which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple alternatively spliced transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.[2]

Interactions

U2AF2 has been shown to interact with:

References

  1. ↑ Zamore PD, Patton JG, Green MR (Mar 1992). "Cloning and domain structure of the mammalian splicing factor U2AF". Nature 355 (6361): 609–14. doi:10.1038/355609a0. PMID 1538748.
  2. ↑ "Entrez Gene: U2AF2 U2 small nuclear RNA auxiliary factor 2".
  3. ↑ 3.0 3.1 3.2 3.3 Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N et al. (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
  4. ↑ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
  5. ↑ Berglund JA, Abovich N, Rosbash M (Mar 1998). "A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition". Genes Dev. 12 (6): 858–67. doi:10.1101/gad.12.6.858. PMC 316625. PMID 9512519.
  6. ↑ Abovich N, Rosbash M (May 1997). "Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals". Cell 89 (3): 403–12. doi:10.1016/s0092-8674(00)80221-4. PMID 9150140.
  7. ↑ Zhang WJ, Wu JY (Oct 1996). "Functional properties of p54, a novel SR protein active in constitutive and alternative splicing". Mol. Cell. Biol. 16 (10): 5400–8. PMC 231539. PMID 8816452.
  8. ↑ Zhang WJ, Wu JY (Feb 1998). "Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing". Mol. Cell. Biol. 18 (2): 676–84. PMC 108778. PMID 9447963.
  9. ↑ Wang HY, Lin W, Dyck JA, Yeakley JM, Songyang Z, Cantley LC et al. (Feb 1998). "SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells". J. Cell Biol. 140 (4): 737–50. doi:10.1083/jcb.140.4.737. PMC 2141757. PMID 9472028.
  10. ↑ Zhang M, Zamore PD, Carmo-Fonseca M, Lamond AI, Green MR (Sep 1992). "Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit". Proc. Natl. Acad. Sci. U.S.A. 89 (18): 8769–73. doi:10.1073/pnas.89.18.8769. PMC 50002. PMID 1388271.
  11. ↑ Davies RC, Calvio C, Bratt E, Larsson SH, Lamond AI, Hastie ND (Oct 1998). "WT1 interacts with the splicing factor U2AF65 in an isoform-dependent manner and can be incorporated into spliceosomes". Genes Dev. 12 (20): 3217–25. doi:10.1101/gad.12.20.3217. PMC 317218. PMID 9784496.


Further reading