Thionin

Plant thionin

Wheat beta-purothionin. Alpha helices in red, beta sheets in blue, disulphide bridges in yellow. PDB 1BHP
Identifiers
Symbol Thionin
Pfam PF00321
InterPro IPR001010
PROSITE PDOC00244
SCOP 1cnb
SUPERFAMILY 1cnb
TCDB 1.C.44
OPM superfamily 149
OPM protein 2plh
Not to be confused with Thionine or Gamma thionin.

Thionins (without an e) are a family of small proteins found solely in higher plants. Typically, a thionin consists of 45–48 amino acid residues. 6–8 of these are cysteine forming 3–4 disulfide bonds. Some thionins have cytotoxic activity and they are therefore interesting in the development of new drugs against cancer with novel action mechanisms.[1] No thionin has yet been developed into an anti-cancer drug. Alpha- and beta- thionins are related to each other. Gamma thionins have a similar structure but are an unrelated class of protein, now called plant defensins.

The proteins are toxic to animal cells, presumably attacking the cell membrane and rendering it permeable: this results in the inhibition of sugar uptake and allows potassium and phosphate ions, proteins, and nucleotides to leak from cells.[2] Thionins are mainly found in seeds where they may act as a defence against consumption by animals. A barley (Hordeum vulgare) leaf thionin that is highly toxic to plant pathogens and is involved in the mechanism of plant defence against microbial infections has also been identified.[3] The hydrophobic protein crambin from the Abyssinian kale (Crambe abyssinica) is also a member of the thionin family.[2]

Databases

A database for antimicrobial peptides, including thionins is available: PhytAMP.[4]

See also

References

  1. ↑ Florack DE, Stiekema WJ (October 1994). "Thionins: properties, possible biological roles and mechanisms of action". Plant Mol. Biol. 26 (1): 25–37. doi:10.1007/BF00039517. PMID 7948874.
  2. ↑ 2.0 2.1 Vernon LP, Evett GE, Zeikus RD, Gray WR (1985). "A toxic thionin from Pyrularia pubera: purification, properties, and amino acid sequence". Arch. Biochem. Biophys. 238 (1): 18–29. doi:10.1016/0003-9861(85)90136-5. PMID 3985614.
  3. ↑ Apel K, Andresen I, Becker W, Schluter K, Burges J, Parthier B (1992). "The identification of leaf thionin as one of the main jasmonate-induced proteins of barley (Hordeum vulgare)". Plant Mol. Biol. 19 (2): 193–204. doi:10.1007/BF00027341. PMID 1377959.
  4. ↑ "PhytAMP Database".; Hammami R, Ben Hamida J, Vergoten G, Fliss I (January 2009). "PhytAMP: a database dedicated to antimicrobial plant peptides". Nucleic Acids Res. 37 (Database issue): D963–8. doi:10.1093/nar/gkn655. PMC 2686510. PMID 18836196.