TRIM45

Tripartite motif containing 45
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
SymbolsTRIM45 ; RNF99
External IDsOMIM: 609318 HomoloGene: 11865 GeneCards: TRIM45 Gene
Orthologs
SpeciesHumanMouse
Entrez80263229644
EnsemblENSG00000134253ENSMUSG00000033233
UniProtQ9H8W5Q6PFY8
RefSeq (mRNA)NM_001145635NM_001165952
RefSeq (protein)NP_001139107NP_001159424
Location (UCSC)Chr 1:
117.65 – 117.67 Mb		Chr 3:
100.92 – 100.94 Mb
PubMed search

tripartite motif containing 45, also known as TRIM45, is a human gene.[1]

This gene encodes a member of the tripartite motif family. The encoded protein may function as a [transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.[1]

Model organisms

Model organisms have been used in the study of TRIM45 function. A conditional knockout mouse line, called Trim45tm1a(KOMP)Wtsi[7][8] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[9][10][11]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[5][12] Twenty six tests were carried out on mutant mice and three significant abnormalities were observed.[5] No homozygous mutant embryos were identified during gestation, and therefore none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice; males had increased circulating magnesium levels while animals of both sex displayed increased bone strength.[5]

References

  1. ↑ 1.0 1.1 "Entrez Gene: tripartite motif containing 45". Retrieved 2011-08-30.
  2. ↑ "Clinical chemistry data for Trim45". Wellcome Trust Sanger Institute.
  3. ↑ "Salmonella infection data for Trim45". Wellcome Trust Sanger Institute.
  4. ↑ "Citrobacter infection data for Trim45". Wellcome Trust Sanger Institute.
  5. ↑ 5.0 5.1 5.2 5.3 Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
  6. ↑ Mouse Resources Portal, Wellcome Trust Sanger Institute.
  7. ↑ "International Knockout Mouse Consortium".
  8. ↑ "Mouse Genome Informatics".
  9. ↑ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
  10. ↑ Dolgin E (2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
  11. ↑ Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
  12. ↑ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading