TPX2

TPX2, microtubule-associated

Rendering of Aurora A kinase bound to a 43 residue peptide fragment of TPX2 from PDB 1OL5

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
1OL5, 3E5A, 3HA6

Identifiers

TPX2 ; C20orf1; C20orf2; DIL-2; DIL2; FLS353; GD:C20orf1; HCA519; HCTP4; REPP86; p100

External IDs

OMIM: 605917 MGI: 1919369 HomoloGene: 8107 ChEMBL: 5389 GeneCards: TPX2 Gene

Gene ontology
Molecular function	• protein binding • ATP binding • GTP binding • protein kinase binding
Cellular component	• spindle pole • nucleus • nucleolus • cytoplasm • spindle • microtubule • microtubule cytoskeleton
Biological process	• apoptotic process • mitotic nuclear division • cell proliferation • activation of protein kinase activity • regulation of mitotic spindle organization
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

ENSG00000088325

ENSMUSG00000027469

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 20:
30.33 – 30.39 Mb

Chr 2:
152.85 – 152.9 Mb

PubMed search

Targeting protein for Xklp2 is a protein that in humans is encoded by the TPX2 gene.^[1]^[2]^[3]

Interactions

TPX2 has been shown to interact with Aurora A kinase ^[4] and KIF15

References

↑ Heidebrecht HJ, Buck F, Steinmann J, Sprenger R, Wacker HH, Parwaresch R (Jul 1997). "p100: a novel proliferation-associated nuclear protein specifically restricted to cell cycle phases S, G2, and M". Blood 90 (1): 226–33. PMID 9207457.
↑ Zhang Y, Heidebrecht H, Rott A, Schlegelberger B, Parwaresch R (Jul 1999). "Assignment of human proliferation associated p100 gene (C20orf1) to human chromosome band 20q11.2 by in situ hybridization". Cytogenet Cell Genet 84 (3–4): 182–3. doi:10.1159/000015251. PMID 10393424.
↑ "Entrez Gene: TPX2 TPX2, microtubule-associated, homolog (Xenopus laevis)".
↑ Kufer, Thomas A; Silljé Herman H W; Körner Roman; Gruss Oliver J; Meraldi Patrick; Nigg Erich A (Aug 2002). "Human TPX2 is required for targeting Aurora-A kinase to the spindle". J. Cell Biol. (United States) 158 (4): 617–23. doi:10.1083/jcb.200204155. ISSN 0021-9525. PMC 2174010. PMID 12177045.

Further reading

Manda R, Kohno T, Matsuno Y et al. (1999). "Identification of genes (SPON2 and C20orf2) differentially expressed between cancerous and noncancerous lung cells by mRNA differential display". Genomics 61 (1): 5–14. doi:10.1006/geno.1999.5939. PMID 10512675.
Wittmann T, Wilm M, Karsenti E, Vernos I (2000). "TPX2, A novel xenopus MAP involved in spindle pole organization". J. Cell Biol. 149 (7): 1405–18. doi:10.1083/jcb.149.7.1405. PMC 2175143. PMID 10871281.
Deloukas P, Matthews LH, Ashurst J et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20". Nature 414 (6866): 865–71. doi:10.1038/414865a. PMID 11780052.
Wang Y, Han KJ, Pang XW et al. (2002). "Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies". J. Immunol. 169 (2): 1102–9. doi:10.4049/jimmunol.169.2.1102. PMID 12097419.
Kufer TA, Silljé HH, Körner R et al. (2002). "Human TPX2 is required for targeting Aurora-A kinase to the spindle". J. Cell Biol. 158 (4): 617–23. doi:10.1083/jcb.200204155. PMC 2174010. PMID 12177045.
Gruss OJ, Wittmann M, Yokoyama H et al. (2004). "Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells". Nat. Cell Biol. 4 (11): 871–9. doi:10.1038/ncb870. PMID 12389033.
Garrett S, Auer K, Compton DA, Kapoor TM (2003). "hTPX2 is required for normal spindle morphology and centrosome integrity during vertebrate cell division". Curr. Biol. 12 (23): 2055–9. doi:10.1016/S0960-9822(02)01277-0. PMID 12477396.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Heidebrecht HJ, Adam-Klages S, Szczepanowski M et al. (2004). "repp86: A human protein associated in the progression of mitosis". Mol. Cancer Res. 1 (4): 271–9. PMID 12612055.
Bayliss R, Sardon T, Vernos I, Conti E (2003). "Structural basis of Aurora-A activation by TPX2 at the mitotic spindle". Mol. Cell 12 (4): 851–62. doi:10.1016/S1097-2765(03)00392-7. PMID 14580337.
Ota T, Suzuki Y, Nishikawa T et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Cassimeris L, Morabito J (2004). "TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly". Mol. Biol. Cell 15 (4): 1580–90. doi:10.1091/mbc.E03-07-0544. PMC 379257. PMID 14718566.
Beausoleil SA, Jedrychowski M, Schwartz D et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
Gerhard DS, Wagner L, Feingold EA et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Maxwell CA, Keats JJ, Belch AR et al. (2005). "Receptor for hyaluronan-mediated motility correlates with centrosome abnormalities in multiple myeloma and maintains mitotic integrity". Cancer Res. 65 (3): 850–60. PMID 15705883.
Stewart S, Fang G (2005). "Anaphase-promoting complex/cyclosome controls the stability of TPX2 during mitotic exit". Mol. Cell. Biol. 25 (23): 10516–27. doi:10.1128/MCB.25.23.10516-10527.2005. PMC 1291225. PMID 16287863.
Ma Y, Lin D, Sun W et al. (2006). "Expression of targeting protein for xklp2 associated with both malignant transformation of respiratory epithelium and progression of squamous cell lung cancer". Clin. Cancer Res. 12 (4): 1121–7. doi:10.1158/1078-0432.CCR-05-1766. PMID 16489064.
Nousiainen M, Silljé HH, Sauer G et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.

PDB gallery

1ol5: STRUCTURE OF AURORA-A 122-403, PHOSPHORYLATED ON THR287, THR288 AND BOUND TO TPX2 1-43