Sodium orthovanadate

Sodium orthovanadate
Names
IUPAC name
Sodium vanadate(V)
Other names
sodium vanadate (V),
sodium vanadium oxide
Identifiers
13721-39-6 Yes
ChEMBL ChEMBL179166 
Jmol-3D images Image
PubChem 61671
RTECS number YW1120000
Properties
Na3VO4
Molar mass 183.908 g/mol
Appearance white powder
Density 2.16 g/cm³, solid
Melting point 858 °C (1,576 °F; 1,131 K)
22.17 g/100 mL
Solubility insoluble in ethanol
Structure
Crystal structure cubic
Thermochemistry
Specific
heat capacity (C)
 164.8 J/mol K
190 J/mol K
Std enthalpy of
formation (ΔfHo298)
 -1757 kJ/mol
Hazards
Main hazards Harmful.
NFPA 704
Flammability code 1: Must be pre-heated before ignition can occur. Flash point over 93 °C (200 °F). E.g., canola oil Health code 2: Intense or continued but not chronic exposure could cause temporary incapacitation or possible residual injury. E.g., chloroform Reactivity code 0: Normally stable, even under fire exposure conditions, and is not reactive with water. E.g., liquid nitrogen Special hazards (white): no codeNFPA 704 four-colored diamond
1
2
0
Flash point Non-flammable
330 mg/kg (oral, rat)
Except where noted otherwise, data is given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
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Infobox references

Sodium orthovanadate is the chemical compound Na3VO4 and contains the tetrahedral VO43−.[1] It is an inhibitor of protein tyrosine phosphatases, alkaline phosphatases and a number of ATPases, most likely acting as a phosphate analogue. The VO43− ion binds reversibly to the active sites of most protein tyrosine phosphatases.

It is often added to buffer solutions that are used in protein analysis in molecular biology. The aim is to preserve the phosphorylation of proteins of interest by inhibiting endogenous phosphatases present in cell lysate mixture. It is commonly used at a final working concentration of 1 - 10 mM. It is toxic when inhaled, swallowed or in contact with skin.

Synthesis

Sodium orthovanadate is created by dissolving vanadium(V) oxide in a solution of sodium hydroxide.

References

  1. ↑ Greenwood, Norman N.; Earnshaw, Alan (1997). Chemistry of the Elements (2nd ed.). Butterworth-Heinemann. ISBN 0080379419.
Swarup, G. et al. Biochem. Biophys. Res. Comm. 107: 1104-1109 (1982).

See also