Small nucleolar RNA SNORD45

Small nucleolar RNA SNORD45
Predicted secondary structure and sequence conservation of SNORD45
Identifiers
Symbol SNORD45
Alt. Symbols U45
Rfam RF00279
Other data
RNA type Gene; snRNA; snoRNA; C/D-box
Domain(s) Eukaryota
GO 0006396 0005730
SO 0000593

snoRNA U45 (also known as SNORD45) is a non-coding RNA (ncRNA) molecule which functions in the modification of other small nuclear RNAs (snRNAs). This type of modifying RNA is usually located in the nucleolus of the eukaryotic cell which is a major site of snRNA biogenesis. It is known as a small nucleolar RNA (snoRNA) and also often referred to as a guide RNA.

snoRNA U45 belongs to the C/D box class of snoRNAs which contain the conserved sequence motifs known as the C box (UGAUGA) and the D box (CUGA). Most of the members of the box C/D family function in directing site-specific 2'-O-methylation of substrate RNAs.[1]

U45 was originally cloned from HeLa cells and expression verified by northern blotting.[2] It is related to the snoRNA (MBII-401) identified in mouse[3]

In the human genome there are three very closely related copies of snoRNA U45 (called U45A, U45B, U45C) located within the introns of the same host gene.[4]

References

  1. ↑ Galardi, S.; Fatica, A.; Bachi, A.; Scaloni, A.; Presutti, C.; Bozzoni, I. (October 2002). "Purified Box C/D snoRNPs Are Able to Reproduce Site-Specific 2'-O-Methylation of Target RNA in Vitro". Molecular and Cellular Biology 22 (19): 6663–6668. doi:10.1128/MCB.22.19.6663-6668.2002. PMC 134041. PMID 12215523.
  2. ↑ Kiss-László, Zsuzsanna; Henry, Yves; Bachellerie, Jean-Pierre; Caizergues-Ferrer, Michèle; Kiss, Tamás (June 1996). "Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs". Cell 85 (7): 1077–1088. doi:10.1016/S0092-8674(00)81308-2. PMID 8674114.
  3. ↑ Huttenhofer, Alexander; Kiefmann, Martin; Meier-Ewert, Sebastian; O'Brien, John; Lehrach, Hans; Bachellerie, Jean-Pierre; Brosius, Jürgen (1 June 2001). "RNomics: an experimental approach that identifies 201 candidates for novel, small, non-messenger RNAs in mouse". The EMBO Journal 20 (11): 2943–2953. doi:10.1093/emboj/20.11.2943. PMC 125495. PMID 11387227.
  4. ↑ Lestrade, Laurent; Weber, Michel J. (2006). "snoRNA-LBME-db, a comprehensive database of human H/ACA and C/D box snoRNAs". Nucleic Acids Research 34 (Supplement 1: Database Issue): D158–D162. doi:10.1093/nar/gkj002. PMC 1347365. PMID 16381836. CiteSeerX: 10.1.1.105.7552.

External links