SMYD3
| SET and MYND domain containing 3 | |||||||||||||
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| Identifiers | |||||||||||||
| Symbols | SMYD3 ; KMT3E; ZMYND1; ZNFN3A1; bA74P14.1 | ||||||||||||
| External IDs | OMIM: 608783 MGI: 1916976 HomoloGene: 41491 GeneCards: SMYD3 Gene | ||||||||||||
| EC number | 2.1.1.43 | ||||||||||||
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| RNA expression pattern | |||||||||||||
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| More reference expression data | |||||||||||||
| Orthologs | |||||||||||||
| Species | Human | Mouse | |||||||||||
| Entrez | 64754 | 69726 | |||||||||||
| Ensembl | ENSG00000185420 | ENSMUSG00000055067 | |||||||||||
| UniProt | Q9H7B4 | Q9CWR2 | |||||||||||
| RefSeq (mRNA) | NM_001167740 | NM_027188 | |||||||||||
| RefSeq (protein) | NP_001161212 | NP_081464 | |||||||||||
| Location (UCSC) | Chr 1: 245.91 – 246.67 Mb | Chr 1: 178.95 – 179.52 Mb | |||||||||||
| PubMed search | |||||||||||||
SET and MYND domain-containing protein 3 is a protein that in humans is encoded by the SMYD3 gene.[1]
SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.[1]
Model organisms
| Characteristic | Phenotype |
|---|---|
| Homozygote viability | Normal |
| Body weight | Normal |
| Anxiety | Normal |
| Neurological assessment | Normal |
| Grip strength | Normal |
| Hot plate | Normal |
| Dysmorphology | Normal |
| Indirect calorimetry | Normal |
| Glucose tolerance test | Normal |
| Auditory brainstem response | Normal |
| DEXA | Normal |
| Radiography | Normal |
| Body temperature | Normal |
| Eye morphology | Normal |
| Clinical chemistry | Normal |
| Plasma immunoglobulins | Normal |
| Haematology | Normal |
| Peripheral blood lymphocytes | Normal |
| Micronucleus test | Normal |
| Heart weight | Normal |
| Brain histopathology | Normal |
| Salmonella infection | Normal[2] |
| Citrobacter infection | Normal[3] |
| All tests and analysis from[4][5] |
Model organisms have been used in the study of SMYD3 function. A conditional knockout mouse line, called Smyd3tm2a(KOMP)Wtsi[6][7] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.[8][9][10]
Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[4][11] Twenty three tests were carried out on homozygous mutant adult mice, however no significant abnormalities were observed.[4]
Interactions
SMYD3 has been shown to interact with Heat shock protein 90kDa alpha (cytosolic), member A1[12] and POLR2A.[12]
SMYD3 trimethylates a lysine residue on MAP3K2, which causes crosstalk into the MAP kinase signaling pathway in Ras-driven cancers.[13]
References
- ↑ 1.0 1.1 "Entrez Gene: SMYD3 SET and MYND domain containing 3".
- ↑ "Salmonella infection data for Smyd3". Wellcome Trust Sanger Institute.
- ↑ "Citrobacter infection data for Smyd3". Wellcome Trust Sanger Institute.
- ↑ 4.0 4.1 4.2 Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x.
- ↑ Mouse Resources Portal, Wellcome Trust Sanger Institute.
- ↑ "International Knockout Mouse Consortium".
- ↑ "Mouse Genome Informatics".
- ↑ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
- ↑ Dolgin E (June 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- ↑ Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
- ↑ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
- ↑ 12.0 12.1 Hamamoto, Ryuji; Furukawa Yoichi; Morita Masashi; Iimura Yuko; Silva Fabio Pittella; Li Meihua; Yagyu Ryuichiro; Nakamura Yusuke (August 2004). "SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells". Nat. Cell Biol. (England) 6 (8): 731–40. doi:10.1038/ncb1151. ISSN 1465-7392. PMID 15235609.
- ↑ Mazur, Pawel K.; Nicolas Reynoird; Purvesh Khatri; Pascal W. T. C. Jansen; Alex W. Wilkinson; Shichong Liu; Olena Barbash; Glenn S. Van Aller; Michael Huddleston; Dashyant Dhanak; Peter J. Tummino; Ryan G. Kruger; Benjamin A. Garcia; Atul J. Butte; Michiel Vermeulen; Julien Sage; Or Gozani (June 2014). "SMYD3 links lysine methylation of MAP3K2 to Ras-driven cancer". Nature 510 (7504): 283–87. doi:10.1038/nature13320. PMID 24847881.
Further reading
- Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Lehner B, Semple JI, Brown SE et al. (2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics 83 (1): 153–67. doi:10.1016/S0888-7543(03)00235-0. PMID 14667819.
- Ota T, Suzuki Y, Nishikawa T et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Hamamoto R, Furukawa Y, Morita M et al. (2004). "SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells". Nat. Cell Biol. 6 (8): 731–40. doi:10.1038/ncb1151. PMID 15235609.
- Gerhard DS, Wagner L, Feingold EA et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Zhou Z, Ren X, Huang X et al. (2006). "SMYD3-NY, a novel SMYD3 mRNA transcript variant, may have a role in human spermatogenesis". Ann. Clin. Lab. Sci. 35 (3): 270–7. PMID 16081583.
- Tsuge M, Hamamoto R, Silva FP et al. (2005). "A variable number of tandem repeats polymorphism in an E2F-1 binding element in the 5' flanking region of SMYD3 is a risk factor for human cancers". Nat. Genet. 37 (10): 1104–7. doi:10.1038/ng1638. PMID 16155568.
- Rual JF, Venkatesan K, Hao T et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
- Hamamoto R, Silva FP, Tsuge M et al. (2006). "Enhanced SMYD3 expression is essential for the growth of breast cancer cells". Cancer Sci. 97 (2): 113–8. doi:10.1111/j.1349-7006.2006.00146.x. PMID 16441421.
- Gregory SG, Barlow KF, McLay KE et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature 441 (7091): 315–21. doi:10.1038/nature04727. PMID 16710414.
- Ewing RM, Chu P, Elisma F et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
- Wang XQ, Miao X, Cai Q et al. (2007). "SMYD3 tandem repeats polymorphism is not associated with the occurrence and metastasis of hepatocellular carcinoma in a Chinese population". Exp. Oncol. 29 (1): 71–3. PMID 17431393.