SGOL1
Shugoshin-like 1 is a protein that in humans is encoded by the SGOL1 gene.[1][2]
Model organisms
| Characteristic | Phenotype |
|---|---|
| Homozygote viability | Abnormal |
| Recessive lethal study | Abnormal |
| Body weight | Normal |
| Anxiety | Normal |
| Neurological assessment | Normal |
| Grip strength | Normal |
| Hot plate | Normal |
| Dysmorphology | Normal |
| Indirect calorimetry | Normal |
| Glucose tolerance test | Normal |
| Auditory brainstem response | Normal |
| DEXA | Normal |
| Radiography | Normal |
| Body temperature | Normal |
| Eye morphology | Normal |
| Clinical chemistry | Normal |
| Plasma immunoglobulins | Normal |
| Haematology | Normal |
| Peripheral blood lymphocytes | Abnormal[3] |
| Micronucleus test | Normal |
| Heart weight | Normal |
| Skin Histopathology | Normal |
| Brain histopathology | Normal |
| Eye Histopathology | Normal |
| Salmonella infection | Normal[4] |
| Citrobacter infection | Normal[5] |
| All tests and analysis from[6][7] |
Model organisms have been used in the study of SGOL1 function. A conditional knockout mouse line, called Sgol1tm1a(EUCOMM)Wtsi[8][9] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[10][11][12]
Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[6][13] Twenty six tests were carried out on mutant mice and three significant abnormalities were observed. No homozygous mutant embryos were identified during gestation, and thus none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice and a decreased regulatory T cell number was observed in male animals.[6][6]
References
- ↑ Scanlan MJ, Gout I, Gordon CM, Williamson B, Stockert E, Gure AO, Jager D, Chen YT, Mackay A, O'Hare MJ, Old LJ (May 2003). "Humoral immunity to human breast cancer: antigen definition and quantitative analysis of mRNA expression". Cancer Immun 1: 4. PMID 12747765.
- ↑ "Entrez Gene: SGOL1 shugoshin-like 1 (S. pombe)".
- ↑ "Peripheral blood lymphocytes data for Sgol1". Wellcome Trust Sanger Institute.
- ↑ "Salmonella infection data for Sgol1". Wellcome Trust Sanger Institute.
- ↑ "Citrobacter infection data for Sgol1". Wellcome Trust Sanger Institute.
- ↑ 6.0 6.1 6.2 6.3 Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
- ↑ Mouse Resources Portal, Wellcome Trust Sanger Institute.
- ↑ "International Knockout Mouse Consortium".
- ↑ "Mouse Genome Informatics".
- ↑ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
- ↑ Dolgin E (2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- ↑ Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
- ↑ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
Further reading
- Wang X, Dai W (2005). "Shugoshin, a guardian for sister chromatid segregation.". Exp. Cell Res. 310 (1): 1–9. doi:10.1016/j.yexcr.2005.07.018. PMID 16112668.
- Fu G, Ding X, Yuan K et al. (2007). "Phosphorylation of human Sgo1 by NEK2A is essential for chromosome congression in mitosis.". Cell Res. 17 (7): 608–18. doi:10.1038/cr.2007.55. PMID 17621308.
- Pouwels J, Kukkonen AM, Lan W et al. (2007). "Shugoshin 1 plays a central role in kinetochore assembly and is required for kinetochore targeting of Plk1.". Cell Cycle 6 (13): 1579–85. doi:10.4161/cc.6.13.4442. PMID 17617734.
- Fu G, Hua S, Ward T et al. (2007). "D-box is required for the degradation of human Shugoshin and chromosome alignment.". Biochem. Biophys. Res. Commun. 357 (3): 672–8. doi:10.1016/j.bbrc.2007.03.204. PMID 17448445.
- Yang Y, Wang X, Dai W (2006). "Human Sgo1 is an excellent target for induction of apoptosis of transformed cells.". Cell Cycle 5 (8): 896–901. doi:10.4161/cc.5.8.2691. PMID 16628005.
- Tang Z, Shu H, Qi W et al. (2006). "PP2A is required for centromeric localization of Sgo1 and proper chromosome segregation.". Dev. Cell 10 (5): 575–85. doi:10.1016/j.devcel.2006.03.010. PMID 16580887.
- Kitajima TS, Sakuno T, Ishiguro K et al. (2006). "Shugoshin collaborates with protein phosphatase 2A to protect cohesin.". Nature 441 (7089): 46–52. doi:10.1038/nature04663. PMID 16541025.
- McGuinness BE, Hirota T, Kudo NR et al. (2006). "Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells.". PLoS Biol. 3 (3): e86. doi:10.1371/journal.pbio.0030086. PMC 1054882. PMID 15737064.
- Kitajima TS, Hauf S, Ohsugi M et al. (2005). "Human Bub1 defines the persistent cohesion site along the mitotic chromosome by affecting Shugoshin localization.". Curr. Biol. 15 (4): 353–9. doi:10.1016/j.cub.2004.12.044. PMID 15723797.
- Tang Z, Sun Y, Harley SE et al. (2005). "Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis.". Proc. Natl. Acad. Sci. U.S.A. 101 (52): 18012–7. doi:10.1073/pnas.0408600102. PMC 539817. PMID 15604152.
- Gerhard DS, Wagner L, Feingold EA et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.