RuvB-like 1

RuvB-like AAA ATPase 1

PDB rendering based on 2c9o.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
2C9O, 2XSZ

Identifiers

Symbols

RUVBL1 ; ECP54; INO80H; NMP238; PONTIN; Pontin52; RVB1; TIH1; TIP49; TIP49A

External IDs

OMIM: 603449 MGI: 1928760 HomoloGene: 37839 GeneCards: RUVBL1 Gene

EC number

3.6.4.12

Gene ontology
Molecular function	• DNA helicase activity • protein binding • ATP binding • ATPase activity
Cellular component	• Swr1 complex • nucleus • nucleoplasm • nucleolus • cytoplasm • Golgi apparatus • microtubule organizing center • membrane • nuclear matrix • Ino80 complex • NuA4 histone acetyltransferase complex • intracellular membrane-bounded organelle • extracellular vesicular exosome • MLL1 complex
Biological process	• DNA repair • DNA recombination • chromatin organization • nucleosome assembly • transcription, DNA-templated • regulation of transcription from RNA polymerase II promoter • mitotic nuclear division • spermatogenesis • DNA duplex unwinding • centromere-specific nucleosome assembly • regulation of growth • histone H4 acetylation • histone H2A acetylation
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 3:
127.78 – 127.87 Mb

Chr 6:
88.47 – 88.5 Mb

PubMed search

RuvB-like 1 (E. coli), also known as RUVBL1 and TIP49, is a human gene.^[1] RUVBL1 can form a hexamer. The hexamer can form a dodecamer with RUVBL2 protein.^[2] Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity.

Interactions

RuvB-like 1 has been shown to interact with:

References

↑ "Entrez Gene: RUVBL1 RuvB-like 1 (E. coli)".
↑ Puri T, Wendler P, Sigala B, Saibil H, Tsaneva IR (February 2007). "Dodecameric structure and ATPase activity of the human TIP48/TIP49 complex". J. Mol. Biol. 366 (1): 179–92. doi:10.1016/j.jmb.2006.11.030. PMID 17157868.
↑ 3.0 3.1 Park J, Wood MA, Cole MD (March 2002). "BAF53 forms distinct nuclear complexes and functions as a critical c-Myc-interacting nuclear cofactor for oncogenic transformation". Mol. Cell. Biol. 22 (5): 1307–16. doi:10.1128/mcb.22.5.1307-1316.2002. PMC 134713. PMID 11839798.
↑ Bauer A, Huber O, Kemler R (Dec 1998). "Pontin52, an interaction partner of beta-catenin, binds to the TATA box binding protein". Proc. Natl. Acad. Sci. U.S.A. 95 (25): 14787–92. doi:10.1073/pnas.95.25.14787. PMC 24527. PMID 9843967.
↑ 5.0 5.1 Fuchs M, Gerber J, Drapkin R, Sif S, Ikura T, Ogryzko V et al. (August 2001). "The p400 complex is an essential E1A transformation target". Cell 106 (3): 297–307. doi:10.1016/s0092-8674(01)00450-0. PMID 11509179.
↑ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S et al. "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

Doyon Y, Côté J (2004). "The highly conserved and multifunctional NuA4 HAT complex.". Curr. Opin. Genet. Dev. 14 (2): 147–54. doi:10.1016/j.gde.2004.02.009. PMID 15196461. Vancouver style error (help)
Dawson SJ, White LA (1992). "Treatment of Haemophilus aphrophilus endocarditis with ciprofloxacin.". J. Infect. 24 (3): 317–20. doi:10.1016/S0163-4453(05)80037-4. PMID 1602151.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Kanemaki M, Makino Y, Yoshida T, Kishimoto T, Koga A, Yamamoto K et al. (1997). "Molecular cloning of a rat 49-kDa TBP-interacting protein (TIP49) that is highly homologous to the bacterial RuvB". Biochem. Biophys. Res. Commun. 235 (1): 64–8. doi:10.1006/bbrc.1997.6729. PMID 9196036.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Makino Y, Mimori T, Koike C, Kanemaki M, Kurokawa Y, Inoue S et al. (1998). "TIP49, homologous to the bacterial DNA helicase RuvB, acts as an autoantigen in human". Biochem. Biophys. Res. Commun. 245 (3): 819–23. doi:10.1006/bbrc.1998.8504. PMID 9588198.
Qiu XB, Lin YL, Thome KC, Pian P, Schlegel BP, Weremowicz S et al. (1998). "An eukaryotic RuvB-like protein (RUVBL1) essential for growth". J. Biol. Chem. 273 (43): 27786–93. doi:10.1074/jbc.273.43.27786. PMID 9774387.
Holzmann K, Gerner C, Korosec T, Pöltl A, Grimm R, Sauermann G (1998). "Identification and characterization of the ubiquitously occurring nuclear matrix protein NMP 238". Biochem. Biophys. Res. Commun. 252 (1): 39–45. doi:10.1006/bbrc.1998.9604. PMID 9813143. Vancouver style error (help)
Bauer A, Huber O, Kemler R (1999). "Pontin52, an interaction partner of beta-catenin, binds to the TATA box binding protein". Proc. Natl. Acad. Sci. U.S.A. 95 (25): 14787–92. doi:10.1073/pnas.95.25.14787. PMC 24527. PMID 9843967.
Kanemaki M, Kurokawa Y, Matsu-ura T, Makino Y, Masani A, Okazaki K et al. (1999). "TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a". J. Biol. Chem. 274 (32): 22437–44. doi:10.1074/jbc.274.32.22437. PMID 10428817.
Salzer U, Kubicek M, Prohaska R (1999). "Isolation, molecular characterization, and tissue-specific expression of ECP-51 and ECP-54 (TIP49), two homologous, interacting erythroid cytosolic proteins". Biochim. Biophys. Acta 1446 (3): 365–70. doi:10.1016/S0167-4781(99)00104-9. PMID 10524211.
Wood MA, McMahon SB, Cole MD (2000). "An ATPase/helicase complex is an essential cofactor for oncogenic transformation by c-Myc". Mol. Cell 5 (2): 321–30. doi:10.1016/S1097-2765(00)80427-X. PMID 10882073.
Makino Y, Kanemaki M, Koga A, Osano K, Matsu-Ura T, Kurokawa Y et al. (2000). "Chromosome mapping and expression of human tip49 family genes". DNA Seq. 11 (1–2): 145–8. doi:10.3109/10425170009033982. PMID 10902922.
Ikura T, Ogryzko VV, Grigoriev M, Groisman R, Wang J, Horikoshi M et al. (2000). "Involvement of the TIP60 histone acetylase complex in DNA repair and apoptosis". Cell 102 (4): 463–73. doi:10.1016/S0092-8674(00)00051-9. PMID 10966108.
Hawley SB, Tamura T, Miles LA (2001). "Purification, cloning, and characterization of a profibrinolytic plasminogen-binding protein, TIP49a". J. Biol. Chem. 276 (1): 179–86. doi:10.1074/jbc.M004919200. PMID 11027681.
Bauer A, Chauvet S, Huber O, Usseglio F, Rothbächer U, Aragnol D et al. (2001). "Pontin52 and reptin52 function as antagonistic regulators of beta-catenin signalling activity". EMBO J. 19 (22): 6121–30. doi:10.1093/emboj/19.22.6121. PMC 305835. PMID 11080158. Vancouver style error (help)
Park J, Wood MA, Cole MD (2002). "BAF53 forms distinct nuclear complexes and functions as a critical c-Myc-interacting nuclear cofactor for oncogenic transformation". Mol. Cell. Biol. 22 (5): 1307–16. doi:10.1128/MCB.22.5.1307-1316.2002. PMC 134713. PMID 11839798.
Watkins NJ, Dickmanns A, Lührmann R (2003). "Conserved stem II of the box C/D motif is essential for nucleolar localization and is required, along with the 15.5K protein, for the hierarchical assembly of the box C/D snoRNP". Mol. Cell. Biol. 22 (23): 8342–52. doi:10.1128/MCB.22.23.8342-8352.2002. PMC 134055. PMID 12417735. Vancouver style error (help)
Cai Y, Jin J, Tomomori-Sato C, Sato S, Sorokina I, Parmely TJ et al. (2003). "Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex". J. Biol. Chem. 278 (44): 42733–6. doi:10.1074/jbc.C300389200. PMID 12963728.

PDB gallery

2c9o: 3D STRUCTURE OF THE HUMAN RUVB-LIKE HELICASE RUVBL1

RuvB-like 1

Interactions

References

Further reading