RUVBL2

RuvB-like AAA ATPase 2

PDB rendering based on 2cqa.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
2CQA, 2XSZ, 3UK6

Identifiers

Symbols

RUVBL2 ; ECP51; INO80J; REPTIN; RVB2; TIH2; TIP48; TIP49B

External IDs

OMIM: 604788 MGI: 1342299 HomoloGene: 4856 GeneCards: RUVBL2 Gene

EC number

3.6.4.12

Gene ontology
Molecular function	• RNA polymerase II core promoter sequence-specific DNA binding • RNA polymerase II distal enhancer sequence-specific DNA binding • DNA helicase activity • damaged DNA binding • ATP-dependent DNA helicase activity • protein binding • ATP binding • ATPase activity • chromatin DNA binding • identical protein binding • ATP-dependent 5'-3' DNA helicase activity • unfolded protein binding
Cellular component	• Swr1 complex • intracellular • nucleus • nucleoplasm • nuclear euchromatin • nucleolus • cytoplasm • membrane • nuclear matrix • ribonucleoprotein complex • Ino80 complex • NuA4 histone acetyltransferase complex • extracellular vesicular exosome • MLL1 complex
Biological process	• ATP catabolic process • DNA repair • DNA recombination • chromatin organization • chromatin remodeling • transcription, DNA-templated • protein folding • DNA duplex unwinding • cellular response to UV • positive regulation of histone acetylation • regulation of growth • histone H4 acetylation • histone H2A acetylation • positive regulation of transcription from RNA polymerase II promoter • establishment of protein localization to chromatin • cellular response to estradiol stimulus • transcriptional activation by promoter-enhancer looping • negative regulation of estrogen receptor binding
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 19:
49.5 – 49.52 Mb

Chr 7:
45.42 – 45.44 Mb

PubMed search

RuvB-like 2 (E. coli), also known as RUVBL2, is a human gene coding for a protein belonging to the AAA+ family of proteins.

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. However, the evidence for whether RUVBL2 has DNA helicase activity is contradictory.^[1] This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation.^[2]

Interactions

RUVBL2 has been shown to interact with RuvB-like 1^[3] and Activating transcription factor 2.^[4]

References

↑ Puri, T; Wendler, P; Sigala, B; Saibil, H; Tsaneva, IR (2007). "Dodecameric structure and ATPase activity of the human TIP48/TIP49 complex". Journal of Molecular Biology 366 (1): 179–92. doi:10.1016/j.jmb.2006.11.030. PMID 17157868.
↑ "Entrez Gene: RUVBL2 RuvB-like 2 (E. coli)".
↑ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
↑ Cho, S G; Bhoumik A; Broday L; Ivanov V; Rosenstein B; Ronai Z (Dec 2001). "TIP49b, a Regulator of Activating Transcription Factor 2 Response to Stress and DNA Damage". Mol. Cell. Biol. (United States) 21 (24): 8398–413. doi:10.1128/MCB.21.24.8398-8413.2001. ISSN 0270-7306. PMC 100004. PMID 11713276.

Doyon Y, Côté J (2004). "The highly conserved and multifunctional NuA4 HAT complex". Curr. Opin. Genet. Dev. 14 (2): 147–54. doi:10.1016/j.gde.2004.02.009. PMID 15196461.
Kanemaki M, Kurokawa Y, Matsu-ura T et al. (1999). "TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a". J. Biol. Chem. 274 (32): 22437–44. doi:10.1074/jbc.274.32.22437. PMID 10428817.
Salzer U, Kubicek M, Prohaska R (1999). "Isolation, molecular characterization, and tissue-specific expression of ECP-51 and ECP-54 (TIP49), two homologous, interacting erythroid cytosolic proteins". Biochim. Biophys. Acta 1446 (3): 365–70. doi:10.1016/S0167-4781(99)00104-9. PMID 10524211.
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Wood MA, McMahon SB, Cole MD (2000). "An ATPase/helicase complex is an essential cofactor for oncogenic transformation by c-Myc". Mol. Cell 5 (2): 321–30. doi:10.1016/S1097-2765(00)80427-X. PMID 10882073.
Ikura T, Ogryzko VV, Grigoriev M et al. (2000). "Involvement of the TIP60 histone acetylase complex in DNA repair and apoptosis". Cell 102 (4): 463–73. doi:10.1016/S0092-8674(00)00051-9. PMID 10966108.
Parfait B, Giovangrandi Y, Asheuer M et al. (2000). "Human TIP49b/RUVBL2 gene: genomic structure, expression pattern, physical link to the human CGB/LHB gene cluster on chromosome 19q13.3". Ann. Genet. 43 (2): 69–74. doi:10.1016/S0003-3995(00)01016-9. PMID 10998447.
Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Bauer A, Chauvet S, Huber O et al. (2001). "Pontin52 and Reptin52 function as antagonistic regulators of β-catenin signalling activity". EMBO J. 19 (22): 6121–30. doi:10.1093/emboj/19.22.6121. PMC 305835. PMID 11080158.
Wiemann S, Weil B, Wellenreuther R et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
Simpson JC, Wellenreuther R, Poustka A et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
Cho SG, Bhoumik A, Broday L et al. (2001). "TIP49b, a Regulator of Activating Transcription Factor 2 Response to Stress and DNA Damage". Mol. Cell. Biol. 21 (24): 8398–413. doi:10.1128/MCB.21.24.8398-8413.2001. PMC 100004. PMID 11713276.
Park J, Wood MA, Cole MD (2002). "BAF53 Forms Distinct Nuclear Complexes and Functions as a Critical c-Myc-Interacting Nuclear Cofactor for Oncogenic Transformation". Mol. Cell. Biol. 22 (5): 1307–16. doi:10.1128/MCB.22.5.1307-1316.2002. PMC 134713. PMID 11839798.
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Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
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Cai Y, Jin J, Tomomori-Sato C et al. (2003). "Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex". J. Biol. Chem. 278 (44): 42733–6. doi:10.1074/jbc.C300389200. PMID 12963728.
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PDB gallery

2cqa: Solution structure of RSGI RUH-039, a fragment of C-terminal domain of RuvB-like 2 from human cDNA

RUVBL2

Interactions

References

Further reading