RNPS1

RNA binding protein S1, serine-rich domain

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
4A8X

Identifiers

Symbols

RNPS1 ; E5.1

External IDs

OMIM: 606447 HomoloGene: 40648 GeneCards: RNPS1 Gene

Gene ontology
Molecular function	• nucleotide binding • RNA binding • mRNA 3'-UTR binding • protein binding • poly(A) RNA binding
Cellular component	• nucleus • nucleoplasm • cytoplasm • cytosol • nuclear speck • exon-exon junction complex • ASAP complex
Biological process	• nuclear-transcribed mRNA catabolic process, nonsense-mediated decay • regulation of alternative mRNA splicing, via spliceosome • mRNA splicing, via spliceosome • transcription, DNA-templated • transcription from RNA polymerase II promoter • termination of RNA polymerase II transcription • mRNA export from nucleus • RNA splicing • gene expression • RNA metabolic process • mRNA metabolic process • mRNA 3'-end processing • positive regulation of apoptotic process • negative regulation of mRNA splicing, via spliceosome
Sources: Amigo / QuickGO

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 16:
2.3 – 2.32 Mb

Chr 17:
24.41 – 24.43 Mb

PubMed search

RNA-binding protein with serine-rich domain 1 is a protein that in humans is encoded by the RNPS1 gene.^[1]^[2]^[3]

This gene encodes a protein that is part of a post-splicing multiprotein complex, the exon junction complex, involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. This protein contains many serine residues. Two splice variants have been found for this gene; both variants encode the same protein.^[3]

Interactions

RNPS1 has been shown to interact with SART3^[4] and Pinin.^[5]^[6]

References

↑ Loyer P, Trembley JH, Lahti JM, Kidd VJ (July 1998). "The RNP protein, RNPS1, associates with specific isoforms of the p34cdc2-related PITSLRE protein kinase in vivo". J Cell Sci 111 (11): 1495–506. PMID 9580558.
↑ Badolato J, Gardiner E, Morrison N, Eisman J (February 1996). "Identification and characterisation of a novel human RNA-binding protein". Gene 166 (2): 323–7. doi:10.1016/0378-1119(95)00571-4. PMID 8543184.
↑ 3.0 3.1 "Entrez Gene: RNPS1 RNA binding protein S1, serine-rich domain".
↑ Harada, K; Yamada A; Yang D; Itoh K; Shichijo S (September 2001). "Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation". Int. J. Cancer (United States) 93 (5): 623–8. doi:10.1002/ijc.1391. ISSN 0020-7136. PMID 11477570.
↑ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
↑ Li, Chin; Lin Ru-Inn; Lai Ming-Chih; Ouyang Pin; Tarn Woan-Yuh (October 2003). "Nuclear Pnn/DRS Protein Binds to Spliced mRNPs and Participates in mRNA Processing and Export via Interaction with RNPS1". Mol. Cell. Biol. (United States) 23 (20): 7363–76. doi:10.1128/MCB.23.20.7363-7376.2003. ISSN 0270-7306. PMC 230327. PMID 14517304.

Burn TC; Connors TD; Van Raay TJ et al. (1996). "Generation of a transcriptional map for a 700-kb region surrounding the polycystic kidney disease type 1 (PKD1) and tuberous sclerosis type 2 (TSC2) disease genes on human chromosome 16p3.3". Genome Res. 6 (6): 525–37. doi:10.1101/gr.6.6.525. PMID 8828041.
Wilson KF; Fortes P; Singh US et al. (1999). "The nuclear cap-binding complex is a novel target of growth factor receptor-coupled signal transduction". J. Biol. Chem. 274 (7): 4166–73. doi:10.1074/jbc.274.7.4166. PMID 9933612.
Mayeda A; Badolato J; Kobayashi R et al. (1999). "Purification and characterization of human RNPS1: a general activator of pre-mRNA splicing". EMBO J. 18 (16): 4560–70. doi:10.1093/emboj/18.16.4560. PMC 1171530. PMID 10449421.
Le Hir H, Izaurralde E, Maquat LE, Moore MJ (2001). "The spliceosome deposits multiple proteins 20–24 nucleotides upstream of mRNA exon–exon junctions". EMBO J. 19 (24): 6860–9. doi:10.1093/emboj/19.24.6860. PMC 305905. PMID 11118221.
Harada K; Yamada A; Yang D et al. (2001). "Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation". Int. J. Cancer 93 (5): 623–8. doi:10.1002/ijc.1391. PMID 11477570.
Kim VN, Kataoka N, Dreyfuss G (2001). "Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex". Science 293 (5536): 1832–6. doi:10.1126/science.1062829. PMID 11546873.
Lykke-Andersen J, Shu MD, Steitz JA (2001). "Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1". Science 293 (5536): 1836–9. doi:10.1126/science.1062786. PMID 11546874.
Lejeune F, Ishigaki Y, Li X, Maquat LE (2002). "The exon junction complex is detected on CBP80-bound but not eIF4E-bound mRNA in mammalian cells: dynamics of mRNP remodeling". EMBO J. 21 (13): 3536–45. doi:10.1093/emboj/cdf345. PMC 126094. PMID 12093754.
Strausberg RL; Feingold EA; Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
McCracken S; Longman D; Johnstone IL et al. (2004). "An evolutionarily conserved role for SRm160 in 3'-end processing that functions independently of exon junction complex formation". J. Biol. Chem. 278 (45): 44153–60. doi:10.1074/jbc.M306856200. PMID 12944400.
Li C; Lin RI; Lai MC et al. (2003). "Nuclear Pnn/DRS Protein Binds to Spliced mRNPs and Participates in mRNA Processing and Export via Interaction with RNPS1". Mol. Cell. Biol. 23 (20): 7363–76. doi:10.1128/MCB.23.20.7363-7376.2003. PMC 230327. PMID 14517304.
Kataoka N, Dreyfuss G (2004). "A simple whole cell lysate system for in vitro splicing reveals a stepwise assembly of the exon-exon junction complex". J. Biol. Chem. 279 (8): 7009–13. doi:10.1074/jbc.M307692200. PMID 14625303.
Ota T; Suzuki Y; Nishikawa T et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Sakashita E; Tatsumi S; Werner D et al. (2004). "Human RNPS1 and Its Associated Factors: a Versatile Alternative Pre-mRNA Splicing Regulator In Vivo". Mol. Cell. Biol. 24 (3): 1174–87. doi:10.1128/MCB.24.3.1174-1187.2004. PMC 321435. PMID 14729963.
Nott A, Le Hir H, Moore MJ (2004). "Splicing enhances translation in mammalian cells: an additional function of the exon junction complex". Genes Dev. 18 (2): 210–22. doi:10.1101/gad.1163204. PMC 324426. PMID 14752011.
Beausoleil SA; Jedrychowski M; Schwartz D et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
Jin J; Smith FD; Stark C et al. (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660.
Gerhard DS; Wagner L; Feingold EA et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.

RNPS1

Interactions

References

Further reading