RFC5

Replication factor C (activator 1) 5, 36.5kDa
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
SymbolsRFC5 ; RFC36
External IDsOMIM: 600407 HomoloGene: 6730 GeneCards: RFC5 Gene
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez598572151
EnsemblENSG00000111445ENSMUSG00000029363
UniProtP40937Q9D0F6
RefSeq (mRNA)NM_001130112NM_028128
RefSeq (protein)NP_001123584NP_082404
Location (UCSC)Chr 12:
118.45 – 118.47 Mb		Chr 5:
117.38 – 117.39 Mb
PubMed search

Replication factor C subunit 5 is a protein that in humans is encoded by the RFC5 gene.[1][2]

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.[2]

Interactions

RFC5 has been shown to interact with BRD4,[3] RFC2,[4][5] PCNA,[4][6][7] RFC4[4][5][8][9] and CHTF18.[10]

References

  1. ↑ Okumura K, Nogami M, Taguchi H, Dean FB, Chen M, Pan ZQ, Hurwitz J, Shiratori A, Murakami Y, Ozawa K et al. (Jul 1995). "Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23". Genomics 25 (1): 274–8. doi:10.1016/0888-7543(95)80135-9. PMID 7774928.
  2. ↑ 2.0 2.1 "Entrez Gene: RFC5 replication factor C (activator 1) 5, 36.5kDa".
  3. ↑ Maruyama, Tetsuo; Farina Andrea; Dey Anup; Cheong JaeHun; Bermudez Vladimir P; Tamura Tomohiko; Sciortino Selvaggia; Shuman Jon; Hurwitz Jerard; Ozato Keiko (Sep 2002). "A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase". Mol. Cell. Biol. (United States) 22 (18): 6509–20. doi:10.1128/MCB.22.18.6509-6520.2002. ISSN 0270-7306. PMC 135621. PMID 12192049.
  4. ↑ 4.0 4.1 4.2 Cai, J; Gibbs E; Uhlmann F; Phillips B; Yao N; O'Donnell M; Hurwitz J (Jul 1997). "A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme". J. Biol. Chem. (UNITED STATES) 272 (30): 18974–81. doi:10.1074/jbc.272.30.18974. ISSN 0021-9258. PMID 9228079.
  5. ↑ 5.0 5.1 Cai, J; Yao N; Gibbs E; Finkelstein J; Phillips B; O'Donnell M; Hurwitz J (Sep 1998). "ATP hydrolysis catalyzed by human replication factor C requires participation of multiple subunits". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 95 (20): 11607–12. doi:10.1073/pnas.95.20.11607. ISSN 0027-8424. PMC 21688. PMID 9751713.
  6. ↑ Mossi, R; Jónsson Z O; Allen B L; Hardin S H; Hübscher U (Jan 1997). "Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen". J. Biol. Chem. (UNITED STATES) 272 (3): 1769–76. doi:10.1074/jbc.272.3.1769. ISSN 0021-9258. PMID 8999859.
  7. ↑ Ohta, Satoshi; Shiomi Yasushi; Sugimoto Katsunori; Obuse Chikashi; Tsurimoto Toshiki (Oct 2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". J. Biol. Chem. (United States) 277 (43): 40362–7. doi:10.1074/jbc.M206194200. ISSN 0021-9258. PMID 12171929.
  8. ↑ Rual, Jean-François; Venkatesan Kavitha, Hao Tong, Hirozane-Kishikawa Tomoko, Dricot Amélie, Li Ning, Berriz Gabriel F, Gibbons Francis D, Dreze Matija, Ayivi-Guedehoussou Nono, Klitgord Niels, Simon Christophe, Boxem Mike, Milstein Stuart, Rosenberg Jennifer, Goldberg Debra S, Zhang Lan V, Wong Sharyl L, Franklin Giovanni, Li Siming, Albala Joanna S, Lim Janghoo, Fraughton Carlene, Llamosas Estelle, Cevik Sebiha, Bex Camille, Lamesch Philippe, Sikorski Robert S, Vandenhaute Jean, Zoghbi Huda Y, Smolyar Alex, Bosak Stephanie, Sequerra Reynaldo, Doucette-Stamm Lynn, Cusick Michael E, Hill David E, Roth Frederick P, Vidal Marc (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature (England) 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
  9. ↑ Ellison, V; Stillman B (Mar 1998). "Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity". J. Biol. Chem. (UNITED STATES) 273 (10): 5979–87. doi:10.1074/jbc.273.10.5979. ISSN 0021-9258. PMID 9488738.
  10. ↑ Bermudez, Vladimir P; Maniwa Yoshimasa; Tappin Inger; Ozato Keiko; Yokomori Kyoko; Hurwitz Jerard (Sep 2003). "The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA". Proc. Natl. Acad. Sci. U.S.A. (United States) 100 (18): 10237–42. doi:10.1073/pnas.1434308100. ISSN 0027-8424. PMC 193545. PMID 12930902.

Further reading