REV1
| REV1, polymerase (DNA directed) | |||||||||||||
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 Rendering based on PDB 2EBW. | |||||||||||||
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| Identifiers | |||||||||||||
| Symbols | REV1 ; REV1L | ||||||||||||
| External IDs | OMIM: 606134 MGI: 1929074 HomoloGene: 32309 GeneCards: REV1 Gene | ||||||||||||
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| RNA expression pattern | |||||||||||||
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| Orthologs | |||||||||||||
| Species | Human | Mouse | |||||||||||
| Entrez | 51455 | 56210 | |||||||||||
| Ensembl | ENSG00000135945 | ENSMUSG00000026082 | |||||||||||
| UniProt | Q9UBZ9 | Q920Q2 | |||||||||||
| RefSeq (mRNA) | NM_001037872 | NM_019570 | |||||||||||
| RefSeq (protein) | NP_001032961 | NP_062516 | |||||||||||
| Location (UCSC) | Chr 2: 100.02 – 100.11 Mb | Chr 1: 38.05 – 38.13 Mb | |||||||||||
| PubMed search | |||||||||||||
DNA repair protein REV1 is a protein that in humans is encoded by the REV1 gene.[1][2] This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found.[2] Rev1 is a Y family DNA polymerase; it is sometimes referred to as a deoxycytidyl transferase because it only inserts deoxycytidine (dC) across from lesions. Whether G, A, T, C, or an abasic site, Rev1 will always add a C. Rev1 has the ability to always add a C, because it uses an arginine as a template which complements well with C.[3] Yet it is believed that Rev1 rarely uses its polymerase activity, rather it is thought that Rev1's primary role is as a protein landing pad, whereby it helps direct the recruitment of TLS proteins, especially Pol ζ (Rev3/Rev7).
Interactions
REV1 has been shown to interact with MAD2L2.[4] It is believed that Rev1 may interact with PCNA, once ubiquitylated due to a lesion, and help recruit Pol ζ (Rev3/Rev7) a B family polymerase involved in TLS.
References
- ↑ Lin W, Xin H, Zhang Y, Wu X, Yuan F, Wang Z (Dec 1999). "The human REV1 gene codes for a DNA template-dependent dCMP transferase". Nucleic Acids Res 27 (22): 4468–75. doi:10.1093/nar/27.22.4468. PMC 148731. PMID 10536157.
- ↑ 2.0 2.1 "Entrez Gene: REV1 REV1 homolog (S. cerevisiae)".
- ↑ Nair, DT (Sep 30, 2005). "Rev1 employs a novel mechanism of DNA synthesis using a protein template". Science. doi:10.1126/science.1116336. PMID 16195463. Retrieved Feb 6, 2015.
- ↑ Murakumo, Y; Ogura Y; Ishii H; Numata S; Ichihara M; Croce C M; Fishel R; Takahashi M (September 2001). "Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7". J. Biol. Chem. (United States) 276 (38): 35644–51. doi:10.1074/jbc.M102051200. ISSN 0021-9258. PMID 11485998.
Further reading
- Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery.". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
- Wixler V, Laplantine E, Geerts D et al. (1999). "Identification of novel interaction partners for the conserved membrane proximal region of alpha-integrin cytoplasmic domains.". FEBS Lett. 445 (2-3): 351–5. doi:10.1016/S0014-5793(99)00151-9. PMID 10094488.
- Gibbs PE, Wang XD, Li Z et al. (2000). "The function of the human homolog of Saccharomyces cerevisiae REV1 is required for mutagenesis induced by UV light.". Proc. Natl. Acad. Sci. U.S.A. 97 (8): 4186–91. doi:10.1073/pnas.97.8.4186. PMC 18191. PMID 10760286.
- Wixler V, Geerts D, Laplantine E et al. (2000). "The LIM-only protein DRAL/FHL2 binds to the cytoplasmic domain of several alpha and beta integrin chains and is recruited to adhesion complexes.". J. Biol. Chem. 275 (43): 33669–78. doi:10.1074/jbc.M002519200. PMID 10906324.
- Masuda Y, Takahashi M, Tsunekuni N et al. (2001). "Deoxycytidyl transferase activity of the human REV1 protein is closely associated with the conserved polymerase domain.". J. Biol. Chem. 276 (18): 15051–8. doi:10.1074/jbc.M008082200. PMID 11278384.
- Murakumo Y, Ogura Y, Ishii H et al. (2001). "Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7.". J. Biol. Chem. 276 (38): 35644–51. doi:10.1074/jbc.M102051200. PMID 11485998.
- Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Masuda Y, Ohmae M, Masuda K, Kamiya K (2003). "Structure and enzymatic properties of a stable complex of the human REV1 and REV7 proteins.". J. Biol. Chem. 278 (14): 12356–60. doi:10.1074/jbc.M211765200. PMID 12529368.
- Clark DR, Zacharias W, Panaitescu L, McGregor WG (2004). "Ribozyme-mediated REV1 inhibition reduces the frequency of UV-induced mutations in the human HPRT gene.". Nucleic Acids Res. 31 (17): 4981–8. doi:10.1093/nar/gkg725. PMC 212819. PMID 12930947.
- Guo C, Fischhaber PL, Luk-Paszyc MJ et al. (2004). "Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis.". EMBO J. 22 (24): 6621–30. doi:10.1093/emboj/cdg626. PMC 291821. PMID 14657033.
- Ota T, Suzuki Y, Nishikawa T et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Ohashi E, Murakumo Y, Kanjo N et al. (2005). "Interaction of hREV1 with three human Y-family DNA polymerases.". Genes Cells 9 (6): 523–31. doi:10.1111/j.1356-9597.2004.00747.x. PMID 15189446.
- Tissier A, Kannouche P, Reck MP et al. (2005). "Co-localization in replication foci and interaction of human Y-family members, DNA polymerase pol eta and REVl protein.". DNA Repair (Amst.) 3 (11): 1503–14. doi:10.1016/j.dnarep.2004.06.015. PMID 15380106.
- Hillier LW, Graves TA, Fulton RS et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4.". Nature 434 (7034): 724–31. doi:10.1038/nature03466. PMID 15815621.
- Lin X, Okuda T, Trang J, Howell SB (2006). "Human REV1 modulates the cytotoxicity and mutagenicity of cisplatin in human ovarian carcinoma cells.". Mol. Pharmacol. 69 (5): 1748–54. doi:10.1124/mol.105.020446. PMID 16495473.
- Masuda Y, Kamiya K (2006). "Role of single-stranded DNA in targeting REV1 to primer termini.". J. Biol. Chem. 281 (34): 24314–21. doi:10.1074/jbc.M602967200. PMID 16803901.
- Yuasa MS, Masutani C, Hirano A et al. (2006). "A human DNA polymerase eta complex containing Rad18, Rad6 and Rev1; proteomic analysis and targeting of the complex to the chromatin-bound fraction of cells undergoing replication fork arrest.". Genes Cells 11 (7): 731–44. doi:10.1111/j.1365-2443.2006.00974.x. PMID 16824193.