RBPJ

Recombination signal binding protein for immunoglobulin kappa J region

PDB rendering based on 2f8x.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
2F8X, 3NBN, 3V79

Identifiers

Symbols

RBPJ ; AOS3; CBF1; IGKJRB; IGKJRB1; KBF2; RBP-J; RBPJK; RBPSUH; SUH; csl

External IDs

OMIM: 147183 MGI: 96522 HomoloGene: 7511 GeneCards: RBPJ Gene

Gene ontology
Molecular function	• recombinase activity • RNA polymerase II core promoter proximal region sequence-specific DNA binding • RNA polymerase II core promoter sequence-specific DNA binding • RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription • RNA polymerase II repressing transcription factor binding • DNA binding • chromatin binding • sequence-specific DNA binding transcription factor activity • protein binding • protein N-terminus binding
Cellular component	• MAML1-RBP-Jkappa- ICN1 complex • nucleus • nucleoplasm • transcription factor complex • nucleolus • cytoplasm
Biological process	• negative regulation of transcription from RNA polymerase II promoter • angiogenesis • epithelial to mesenchymal transition • blood vessel remodeling • inflammatory response to antigenic stimulus • outflow tract morphogenesis • endocardium morphogenesis • epithelial to mesenchymal transition involved in endocardial cushion formation • cardiac left ventricle morphogenesis • ventricular trabecula myocardium morphogenesis • regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation • DNA recombination • transcription initiation from RNA polymerase II promoter • humoral immune response • Notch signaling pathway • positive regulation of transcription of Notch receptor target • negative regulation of cell proliferation • auditory receptor cell fate commitment • epidermal cell fate specification • gene expression • pituitary gland development • B cell differentiation • keratinocyte differentiation • negative regulation of ossification • positive regulation of BMP signaling pathway • somatic stem cell maintenance • dorsal aorta morphogenesis • atrioventricular canal development • defense response to bacterium • negative regulation of transcription, DNA-templated • positive regulation of transcription from RNA polymerase II promoter • regulation of timing of cell differentiation • sebaceous gland development • hair follicle maturation • positive regulation of cardiac muscle cell proliferation • Clara cell differentiation • labyrinthine layer blood vessel development • arterial endothelial cell fate commitment • Notch signaling involved in heart development • blood vessel lumenization • interleukin-4 secretion • blood vessel endothelial cell fate specification • positive regulation of ERBB signaling pathway • positive regulation of ephrin receptor signaling pathway • positive regulation of cell proliferation involved in heart morphogenesis
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 4:
26.17 – 26.44 Mb

Chr 5:
53.56 – 53.66 Mb

PubMed search

Recombining binding protein suppressor of hairless is a protein that in humans is encoded by the RBPJ gene.^[1]^[2]^[3]

RBPJ^[4] also known as CBF1, is the human homolog for the Drosophila gene Suppressor of Hairless. Its promoter region is classically used to demonstrate Notch1 signaling.^[5]

Interactions

RBPJ has been shown to interact with:

NOTCH1^[6]^[7]
NCOR2,^[8]^[9]
PCAF,^[10]
SND1,^[11]^[12] and
SNW1.^[11]^[13]

References

↑ Amakawa R, Jing W, Ozawa K, Matsunami N, Hamaguchi Y, Matsuda F, Kawaichi M, Honjo T (October 1993). "Human Jk recombination signal binding protein gene (IGKJRB): comparison with its mouse homologue". Genomics 17 (2): 306–15. doi:10.1006/geno.1993.1326. PMID 8406481.
↑ Tang X, Saito-Ohara F, Song J, Koga C, Ugai H, Murakami H, Ikeuchi T, Yokoyama KK (November 1997). "Assignment of the human gene for KBF2/RBP-Jk to chromosome 9p12-13 and 9q13 by fluorescence in situ hybridization". Jpn J Hum Genet 42 (2): 337–41. doi:10.1007/BF02766956. PMID 9290259.
↑ "Entrez Gene: RBPJ recombination signal binding protein for immunoglobulin kappa J region".
↑ "Human Genome Organization: RBPJ Report".
↑ Hsieh JJ, Henkel T, Salmon P, Robey E, Peterson MG, Hayward SD (1996). "Truncated mammalian Notch1 activates CBF1/RBPJk-repressed genes by a mechanism resembling that of Epstein-Barr virus EBNA2.". Molecular and Cellular Biology 16 (3): 952–959. PMC 231077. PMID 8622698.
↑ Nam Y, Weng AP, Aster JC, Blacklow SC (June 2003). "Structural requirements for assembly of the CSL.intracellular Notch1.Mastermind-like 1 transcriptional activation complex". J. Biol. Chem. 278 (23): 21232–9. doi:10.1074/jbc.M301567200. PMID 12644465.
↑ Aster JC, Robertson ES, Hasserjian RP, Turner JR, Kieff E, Sklar J (April 1997). "Oncogenic forms of NOTCH1 lacking either the primary binding site for RBP-Jkappa or nuclear localization sequences retain the ability to associate with RBP-Jkappa and activate transcription". J. Biol. Chem. 272 (17): 11336–43. doi:10.1074/jbc.272.17.11336. PMID 9111040.
↑ Beatus P, Lundkvist J, Oberg C, Pedersen K, Lendahl U (June 2001). "The origin of the ankyrin repeat region in Notch intracellular domains is critical for regulation of HES promoter activity". Mech. Dev. 104 (1-2): 3–20. doi:10.1016/s0925-4773(01)00373-2. PMID 11404076.
↑ Zhou S, Hayward SD (September 2001). "Nuclear localization of CBF1 is regulated by interactions with the SMRT corepressor complex". Mol. Cell. Biol. 21 (18): 6222–32. doi:10.1128/mcb.21.18.6222-6232.2001. PMC 87339. PMID 11509665.
↑ Kurooka H, Honjo T (June 2000). "Functional interaction between the mouse notch1 intracellular region and histone acetyltransferases PCAF and GCN5". J. Biol. Chem. 275 (22): 17211–20. doi:10.1074/jbc.M000909200. PMID 10747963.
↑ 11.0 11.1 Zhou S, Fujimuro M, Hsieh JJ, Chen L, Hayward SD (February 2000). "A role for SKIP in EBNA2 activation of CBF1-repressed promoters". J. Virol. 74 (4): 1939–47. doi:10.1128/jvi.74.4.1939-1947.2000. PMC 111672. PMID 10644367.
↑ Hsieh JJ, Zhou S, Chen L, Young DB, Hayward SD (January 1999). "CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex". Proc. Natl. Acad. Sci. U.S.A. 96 (1): 23–8. doi:10.1073/pnas.96.1.23. PMC 15086. PMID 9874765.
↑ Zhou S, Fujimuro M, Hsieh JJ, Chen L, Miyamoto A, Weinmaster G, Hayward SD (April 2000). "SKIP, a CBF1-associated protein, interacts with the ankyrin repeat domain of NotchIC To facilitate NotchIC function". Mol. Cell. Biol. 20 (7): 2400–10. doi:10.1128/mcb.20.7.2400-2410.2000. PMC 85419. PMID 10713164.

External links

RBPJ protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)

PDB gallery

2f8x: Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence

Transcription factors and intracellular receptors

(1) Basic domains

(1.1) Basic leucine zipper (bZIP)	Activating transcription factor AATF 1 2 3 4 5 6 7 AP-1 c-Fos FOSB FOSL1 FOSL2 JDP2 c-Jun JUNB JunD BACH 1 2 BATF BLZF1 C/EBP α β γ δ ε ζ CREB 1 3 L1 CREM DBP DDIT3 GABPA HLF MAF B F G K NFE 2 L1 L2 L3 NFIL3 NRL NRF 1 2 3 XBP1

(1.2) Basic helix-loop-helix (bHLH)	ATOH1 AhR AHRR ARNT ASCL1 BHLH 2 3 9 ARNTL ARNTL ARNTL2 CLOCK EPAS1 FIGLA HAND 1 2 HES 5 6 HEY 1 2 L HES1 HIF 1A 3A ID 1 2 3 4 LYL1 MESP2 MXD4 MYCL1 MYCN Myogenic regulatory factors MyoD Myogenin MYF5 MYF6 Neurogenins 1 2 3 NeuroD 1 2 NPAS 1 2 3 OLIG 1 2 Pho4 Scleraxis SIM 1 2 TAL 1 2 Twist USF1

(1.3) bHLH-ZIP	AP-4 MAX MXD3 MITF MNT MLX MXI1 Myc SREBP 1 2

(1.4) NF-1	NFI A B C X SMAD R-SMAD 1 2 3 5 9 I-SMAD 6 7 4)

(1.5) RF-X	RFX 1 2 3 4 5 6 ANK

(1.6) Basic helix-span-helix (bHSH)	AP-2 α β γ δ ε

(2) Zinc finger DNA-binding domains

(2.1) Nuclear receptor (Cys₄)

subfamily 1	Thyroid hormone α β CAR FXR LXR α β PPAR α β/δ γ PXR RAR α β γ ROR α β γ Rev-ErbA α β VDR

subfamily 2	COUP-TF (I II Ear-2 HNF4 α γ PNR RXR α β γ Testicular receptor 2 4 TLX

subfamily 3	Steroid hormone Androgen Estrogen α β Glucocorticoid Mineralocorticoid Progesterone Estrogen related α β γ

subfamily 4	NUR NGFIB NOR1 NURR1

subfamily 5	LRH-1 SF1

subfamily 6	GCNF

subfamily 0	DAX1 SHP

(2.2) Other Cys₄

GATA
- 1
- 2
- 3
- 4
- 5
- 6
MTA
- 1
- 2
- 3
TRPS1

(2.3) Cys₂His₂

General transcription factors
- TFIIA
- TFIIB
- TFIID
- TFIIE
  - 1
  - 2
- TFIIF
- 1
- 2
  - TFIIH
  - 1
  - 2
  - 4
  - 2I
  - 3A
  - 3C1
  - 3C2

ATBF1
BCL
- 6
- 11A
- 11B
CTCF
E4F1
EGR
- 1
- 2
- 3
- 4
ERV3
GFI1
GLI-Krüppel family
- 1
- 2
- 3
- REST
- S1
- S2
- YY1
HIC
- 1
- 2
HIVEP
- 1
- 2
- 3
IKZF
- 1
- 2
- 3
ILF
- 2
- 3
KLF
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 11
- 12
- 13
- 14
- 15
- 17
MTF1
MYT1
OSR1
PRDM9
SALL
- 1
- 2
- 3
- 4
SP
- 1
- 2
- 4
- 7
- 8
TSHZ3
WT1
Zbtb7
- 7A
- 7B
ZBTB
- 11
- 16
- 17
- 20
- 32
- 33
- 40
zinc finger
- 3
- 7
- 9
- 10
- 19
- 22
- 24
- 33B
- 34
- 35
- 41
- 43
- 44
- 51
- 74
- 143
- 146
- 148
- 165
- 202
- 217
- 219
- 238
- 239
- 259
- 267
- 268
- 281
- 295
- 300
- 318
- 330
- 346
- 350
- 365
- 366
- 384
- 423
- 451
- 452
- 471
- 593
- 638
- 644
- 649
- 655

(2.4) Cys₆

HIVEP1

(2.5) Alternating composition

AIRE
DIDO1
GRLF1
ING
- 1
- 2
- 4
JARID
- 1A
- 1B
- 1C
- 1D
- 2
JMJD1B

(2.6) WRKY

WRKY

(3) Helix-turn-helix domains

(3.1) Homeodomain	ARX CDX 1 2 CRX CUTL1 DBX 1 2 DLX 3 4 5 EMX 1 2 EN 1 2 FHL 1 2 3 HESX1 HHEX HLX Homeobox A1 A2 A3 A4 A5 A7 A9 A10 A11 A13 B1 B2 B3 B4 B5 B6 B7 B8 B9 B13 C4 C5 C6 C8 C9 C10 C11 C12 C13 D1 D3 D4 D8 D9 D10 D11 D12 D13 HOPX IRX 1 2 3 4 5 6 MKX LMX 1A 1B MEIS 1 2 MEOX2 MNX1 MSX 1 2 NANOG NKX 2-1 2-2 2-3 2-5 3-1 3-2 6-1 6-2 NOBOX PBX 1 2 3 PHF 1 3 6 8 10 16 17 20 21A PHOX 2A 2B PITX 1 2 3 POU domain PIT-1 BRN-3: A B C Octamer transcription factor: 1 2 3/4 6 7 11 OTX 1 2 PDX1 SATB2 SHOX2 SIX 1 2 3 4 5 VAX1 ZEB 1 2

(3.2) Paired box	PAX 1 2 3 4 5 6 7 8 9 PRRX 1 2 RAX

(3.3) Fork head / winged helix	E2F 1 2 3 4 5 FOX proteins A1 A2 A3 C1 C2 D3 D4 E1 E3 F1 G1 H1 I1 J1 J2 K1 K2 L2 M1 N1 N3 O1 O3 O4 P1 P2 P3 P4

(3.4) Heat shock factors	HSF 1 2 4

(3.5) Tryptophan clusters	ELF 2 4 5 EGF ELK 1 3 4 ERF ETS 1 2 ERG SPIB ETV 1 4 5 6 FLI1 Interferon regulatory factors 1 2 3 4 5 6 7 8 MYB MYBL2

(3.6) TEA domain	transcriptional enhancer factor 1 2 3 4

(4) β-Scaffold factors with minor groove contacts

(4.1) Rel homology region	NF-κB NFKB1 NFKB2 REL RELA RELB NFAT C1 C2 C3 C4 5

(4.2) STAT	STAT 1 2 3 4 5 6

(4.3) p53	p53 TBX 1 2 3 5 19 21 22 TBR1 TBR2 TFT MYRF TP63

(4.4) MADS box	Mef2 A B C D SRF

(4.6) TATA-binding proteins	TBP TBPL1

(4.7) High-mobility group	BBX HMGB 1 2 3 4 HMGN 1 2 3 4 HNF 1A 1B LEF1 SOX 1 2 3 4 5 6 8 9 10 11 12 13 14 15 18 21 SRY SSRP1 TCF 3 4 TOX 1 2 3 4

(4.9) Grainyhead	TFCP2

(4.10) Cold-shock domain	CSDA YBX1

(4.11) Runt	CBF CBFA2T2 CBFA2T3 RUNX1 RUNX2 RUNX3 RUNX1T1

(0) Other transcription factors

(0.2) HMGI(Y)	HMGA 1 2 HBP1

(0.3) Pocket domain	Rb RBL1 RBL2

(0.5) AP-2/EREBP-related factors	Apetala 2 EREBP B3

(0.6) Miscellaneous	ARID 1A 1B 2 3A 3B 4A CAP IFI 16 35 MLL 2 3 T1 MNDA NFY A B C Rho/Sigma

see also transcription factor/coregulator deficiencies

Index of genetics

Description	Gene expression DNA replication cycle recombination repair binding proteins Transcription factors regulators nucleic acids RNA RNA binding proteins ribonucleoproteins repeated sequence modification Translation ribosome modification nexins Proteins domains Structure primary secondary tertiary quaternary

Disease	Replication and repair Transcription factor Transcription Translation

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

RBPJ

Interactions

References

Further reading

External links