RACGAP1

Rac GTPase activating protein 1

PDB rendering based on 2ovj.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
2OVJ, 3W6R, 4B6D

Identifiers

Symbols

RACGAP1 ; CYK4; HsCYK-4; ID-GAP; MgcRacGAP

External IDs

OMIM: 604980 MGI: 1349423 HomoloGene: 8077 GeneCards: RACGAP1 Gene

Gene ontology
Molecular function	• GTPase activator activity • protein binding • phosphatidylinositol-3,4,5-trisphosphate binding • microtubule binding • protein kinase binding • alpha-tubulin binding • gamma-tubulin binding • metal ion binding • beta-tubulin binding
Cellular component	• acrosomal vesicle • nucleus • nucleolus • cytosol • microtubule • midbody • extrinsic component of cytoplasmic side of plasma membrane • cleavage furrow • spindle midzone • extracellular vesicular exosome • mitotic spindle • centralspindlin complex
Biological process	• mitotic cytokinesis • actomyosin contractile ring assembly • microtubule-based movement • cytokinesis, initiation of separation • small GTPase mediated signal transduction • spermatogenesis • neuroblast proliferation • blood coagulation • sulfate transport • embryo development • antigen processing and presentation of exogenous peptide antigen via MHC class II • positive regulation of cytokinesis • positive regulation of GTPase activity • regulation of small GTPase mediated signal transduction • spindle midzone assembly involved in mitosis • regulation of attachment of spindle microtubules to kinetochore
Sources: Amigo / QuickGO

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 12:
50.37 – 50.43 Mb

Chr 15:
99.62 – 99.65 Mb

PubMed search

Rac GTPase-activating protein 1 is an enzyme that in humans is encoded by the RACGAP1 gene.^[1]

Function

Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.[supplied by OMIM]^[2]

Interactions

RACGAP1 has been shown to interact with Rnd2^[3] and SLC26A8.^[4]

During cytokinesis, RACGAP1 has been shown to interact with KIF23 to form the centralspindlin complex.^[5]

References

↑ Touré A, Dorseuil O, Morin L, Timmons P, Jégou B, Reibel L et al. (April 1998). "MgcRacGAP, a new human GTPase-activating protein for Rac and Cdc42 similar to Drosophila rotundRacGAP gene product, is expressed in male germ cells". J Biol Chem 273 (11): 6019–23. doi:10.1074/jbc.273.11.6019. PMID 9497316. Vancouver style error (help)
↑ "Entrez Gene: RACGAP1 Rac GTPase activating protein 1".
↑ Naud N, Touré A, Liu J, Pineau C, Morin L, Dorseuil O et al. (May 2003). "Rho family GTPase Rnd2 interacts and co-localizes with MgcRacGAP in male germ cells". Biochem. J. 372 (Pt 1): 105–12. doi:10.1042/BJ20021652. PMC 1223378. PMID 12590651. Vancouver style error (help)
↑ Toure A, Morin L, Pineau C, Becq F, Dorseuil O, Gacon G (June 2001). "Tat1, a novel sulfate transporter specifically expressed in human male germ cells and potentially linked to rhogtpase signaling". J. Biol. Chem. 276 (23): 20309–15. doi:10.1074/jbc.M011740200. PMID 11278976.
↑ Glotzer, Michael. "Cytokinesis: Centralspindlin Moonlights as a Membrane Anchor", Current Biology, 18 February 2013

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Gerwins P, Blank JL, Johnson GL (1997). "Cloning of a novel mitogen-activated protein kinase kinase kinase, MEKK4, that selectively regulates the c-Jun amino terminal kinase pathway.". J. Biol. Chem. 272 (13): 8288–95. doi:10.1074/jbc.272.13.8288. PMID 9079650.
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Nagata K, Puls A, Futter C, Aspenstrom P, Schaefer E, Nakata T et al. (1998). "The MAP kinase kinase kinase MLK2 co-localizes with activated JNK along microtubules and associates with kinesin superfamily motor KIF3.". EMBO J. 17 (1): 149–58. doi:10.1093/emboj/17.1.149. PMC 1170366. PMID 9427749.
Tapon N, Nagata K, Lamarche N, Hall A (1998). "A new rac target POSH is an SH3-containing scaffold protein involved in the JNK and NF-kappaB signalling pathways.". EMBO J. 17 (5): 1395–404. doi:10.1093/emboj/17.5.1395. PMC 1170487. PMID 9482736.
Abo A, Qu J, Cammarano MS, Dan C, Fritsch A, Baud V et al. (1999). "PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia.". EMBO J. 17 (22): 6527–40. doi:10.1093/emboj/17.22.6527. PMC 1171000. PMID 9822598.
Zuber J, Tchernitsa OI, Hinzmann B, Schmitz AC, Grips M, Hellriegel M et al. (2000). "A genome-wide survey of RAS transformation targets.". Nat. Genet. 24 (2): 144–52. doi:10.1038/72799. PMID 10655059.
Das B, Shu X, Day GJ, Han J, Krishna UM, Falck JR et al. (2000). "Control of intramolecular interactions between the pleckstrin homology and Dbl homology domains of Vav and Sos1 regulates Rac binding.". J. Biol. Chem. 275 (20): 15074–81. doi:10.1074/jbc.M907269199. PMID 10748082.
Nagase T, Kikuno R, Ishikawa K, Hirosawa M, Ohara O (2000). "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.". DNA Res. 7 (2): 143–50. doi:10.1093/dnares/7.2.143. PMID 10819331.
Kawashima T, Hirose K, Satoh T, Kaneko A, Ikeda Y, Kaziro Y et al. (2000). "MgcRacGAP is involved in the control of growth and differentiation of hematopoietic cells.". Blood 96 (6): 2116–24. PMID 10979956.
Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination.". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Hirose K, Kawashima T, Iwamoto I, Nosaka T, Kitamura T (2001). "MgcRacGAP is involved in cytokinesis through associating with mitotic spindle and midbody.". J. Biol. Chem. 276 (8): 5821–8. doi:10.1074/jbc.M007252200. PMID 11085985.
Caloca MJ, Wang H, Delemos A, Wang S, Kazanietz MG (2001). "Phorbol esters and related analogs regulate the subcellular localization of beta 2-chimaerin, a non-protein kinase C phorbol ester receptor.". J. Biol. Chem. 276 (21): 18303–12. doi:10.1074/jbc.M011368200. PMID 11278894.
Toure A, Morin L, Pineau C, Becq F, Dorseuil O, Gacon G (2001). "Tat1, a novel sulfate transporter specifically expressed in human male germ cells and potentially linked to rhogtpase signaling.". J. Biol. Chem. 276 (23): 20309–15. doi:10.1074/jbc.M011740200. PMID 11278976.
Mishima M, Kaitna S, Glotzer M (2002). "Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity.". Dev. Cell 2 (1): 41–54. doi:10.1016/S1534-5807(01)00110-1. PMID 11782313.
Vikis HG, Li W, Guan KL (2002). "The plexin-B1/Rac interaction inhibits PAK activation and enhances Sema4D ligand binding.". Genes Dev. 16 (7): 836–45. doi:10.1101/gad.966402. PMC 186329. PMID 11937491.
Kitamura T, Kawashima T, Minoshima Y, Tonozuka Y, Hirose K, Nosaka T (2002). "Role of MgcRacGAP/Cyk4 as a regulator of the small GTPase Rho family in cytokinesis and cell differentiation.". Cell Struct. Funct. 26 (6): 645–51. doi:10.1247/csf.26.645. PMID 11942621.
Côté JF, Vuori K (2003). "Identification of an evolutionarily conserved superfamily of DOCK180-related proteins with guanine nucleotide exchange activity.". J. Cell. Sci. 115 (Pt 24): 4901–13. doi:10.1242/jcs.00219. PMID 12432077. Vancouver style error (help)
Jantsch-Plunger V, Gönczy P, Romano A, Schnabel H, Hamill D, Schnabel R et al. (2000). "CYK-4: A Rho family gtpase activating protein (GAP) required for central spindle formation and cytokinesis.". J Cell Biol 149 (7): 1391–404. doi:10.1083/jcb.149.7.1391. PMC 2175131. PMID 10871280. Vancouver style error (help)

PDB gallery

2ovj: The crystal structure of the human Rac GTPase activating protein 1 (RACGAP1) MgcRacGAP.

RACGAP1

Function

Interactions

References

Further reading