PrimerPlex
| Developer(s) | PREMIER Biosoft |
|---|---|
| Stable release | 2.61 |
| Operating system | Windows, Macintosh |
| Type | Bioinformatics |
| License | commercial |
| Website | www.premierbiosoft.com |
PrimerPlex designs oligos for standard multiplex PCR assays and for bead-based suspension array systems such as BioPlex-200 and Luminex 100/200 based on xMAP technology. The assays are used for high-throughput SNP applications such as genotyping, pathogen detection, strain typing, and haplotyping. It is supported on both Windows and Mac.
The following oligo design options are supported:
- Multiplex PCR Primer Design: Can design highly specific multiplex primers for standard PCR assays for up to 30 sequences.
- Direct Hybridization Assay (DHA) Oligo Design: Can design probes for 100 individual targets in a multiplex reaction. The SNPs are located at the center to maximize discrimination.
- ASPE Assay Oligo Design: Can design primers for 40 individual targets in a multiplex reaction. The mutation is located at the 3' end.
The author company, PREMIER Biosoft, has supported strain differentiation multiplex xMAP assays in one of their other products, AlleleID.
References
- Luminex Corporation
- PREMIER Biosoft International Releases version 2.00 of PrimerPlex with Support for Using Proven Pre-designed oligos and Analyzing SNPs of Medium-sized Genomes
- A multiplex single nucleotide polymorphism typing assay for detecting mutations that result in decreased fluoroquinolone susceptibility in Salmonella enterica serovars Typhi and Paratyphi A