PrfA thermoregulator UTR
| PrfA thermoregulator UTR | |
|---|---|
 | |
| Predicted secondary structure and sequence conservation of PrfA | |
| Identifiers | |
| Symbol | PrfA |
| Rfam | RF00038 |
| Other data | |
| RNA type | Cis-reg; thermoregulator |
| Domain(s) | Bacteria |
| SO | 0000233 |
The PrfA thermoregulator UTR is an RNA thermometer found in the 5' UTR of the prfA gene.[1] In Listeria monocytogenes, virulence genes are maximally expressed at 37°C (human body temperature) but are almost silent at 30°C.[2] The genes are controlled by PrfA, a transcriptional activator whose expression is thermoregulated.[3] It has been shown that the untranslated mRNA (UTR) preceding prfA, forms a secondary structure, which masks the ribosome binding region.[1] It is thought that at 37°C, the hairpin structure 'melts' and the SD sequence is unmasked.[1]
References
- ↑ 1.0 1.1 1.2 Johansson J, Mandin P, Renzoni A, Chiaruttini C, Springer M, Cossart P (2002). "An RNA thermosensor controls expression of virulence genes in Listeria monocytogenes". Cell 110 (5): 551–61. doi:10.1016/S0092-8674(02)00905-4. PMID 12230973.
- ↑ Freitag, NE; Port, GC, Miner, MD (September 2009). "Listeria monocytogenes - from saprophyte to intracellular pathogen.". Nature reviews. Microbiology 7 (9): 623–8. doi:10.1038/nrmicro2171. PMC 2813567. PMID 19648949. Retrieved 10 August 2011.
- ↑ Digel, I; Kayser, P; Artmann, GM (2008). "Molecular processes in biological thermosensation.". Journal of biophysics (Hindawi Publishing Corporation : Online) 2008: 602870. doi:10.1155/2008/602870. PMC 2814129. PMID 20130806. Retrieved 10 August 2011.