POLK

Polymerase (DNA directed) kappa

PDB rendering based on 1t94.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
1T94, 2LSI, 2OH2, 2W7O, 2W7P, 3IN5, 3PZP, 4BA9, 4GK5

Identifiers

Symbols

POLK ; DINB1; DINP; POLQ

External IDs

OMIM: 605650 MGI: 1349767 HomoloGene: 32140 ChEMBL: 5365 GeneCards: POLK Gene

EC number

2.7.7.7

Gene ontology
Molecular function	• damaged DNA binding • DNA-directed DNA polymerase activity • metal ion binding
Cellular component	• nucleus • nucleolus
Biological process	• DNA-dependent DNA replication • DNA repair • nucleotide-excision repair, DNA gap filling
Sources: Amigo / QuickGO

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 5:
75.51 – 75.6 Mb

Chr 13:
96.48 – 96.54 Mb

PubMed search

DNA polymerase kappa is an enzyme that in humans is encoded by the POLK gene.^[1]^[2]^[3]

References

↑ Ohashi E, Ogi T, Kusumoto R, Iwai S, Masutani C, Hanaoka F, Ohmori H (Aug 2000). "Error-prone bypass of certain DNA lesions by the human DNA polymerase κ". Genes Dev 14 (13): 1589–94. PMC 316741. PMID 10887153.
↑ Gerlach VL, Aravind L, Gotway G, Schultz RA, Koonin EV, Friedberg EC (Nov 1999). "Human and mouse homologs of Escherichia coli DinB (DNA polymerase IV), members of the UmuC/DinB superfamily". Proc Natl Acad Sci U S A 96 (21): 11922–7. doi:10.1073/pnas.96.21.11922. PMC 18388. PMID 10518552.
↑ "Entrez Gene: POLK Polymerase (DNA directed) kappa".

Bavoux C, Hoffmann JS, Cazaux C (2005). "Adaptation to DNA damage and stimulation of genetic instability: the double-edged sword mammalian DNA polymerase kappa". Biochimie 87 (7): 637–46. doi:10.1016/j.biochi.2005.02.007. PMID 15989980.
Ogi T, Kato T, Kato T, Ohmori H (2000). "Mutation enhancement by DINB1, a mammalian homologue of the Escherichia coli mutagenesis protein dinB". Genes Cells 4 (11): 607–18. doi:10.1046/j.1365-2443.1999.00289.x. PMID 10620008.
Johnson RE, Prakash S, Prakash L (2000). "The human DINB1 gene encodes the DNA polymerase Polθ". Proc. Natl. Acad. Sci. U.S.A. 97 (8): 3838–43. doi:10.1073/pnas.97.8.3838. PMC 18103. PMID 10760255.
Gerlach VL, Feaver WJ, Fischhaber PL, Friedberg EC (2001). "Purification and characterization of pol kappa, a DNA polymerase encoded by the human DINB1 gene". J. Biol. Chem. 276 (1): 92–8. doi:10.1074/jbc.M004413200. PMID 11024016.
Haracska L, Unk I, Johnson RE et al. (2002). "Stimulation of DNA Synthesis Activity of Human DNA Polymerase κ by PCNA". Mol. Cell. Biol. 22 (3): 784–91. doi:10.1128/MCB.22.3.784-791.2002. PMC 133560. PMID 11784855.
Washington MT, Johnson RE, Prakash L, Prakash S (2002). "Human DINB1-encoded DNA polymerase κ is a promiscuous extender of mispaired primer termini". Proc. Natl. Acad. Sci. U.S.A. 99 (4): 1910–4. doi:10.1073/pnas.032594399. PMC 122293. PMID 11842189.
Suzuki N, Ohashi E, Kolbanovskiy A et al. (2002). "Translesion synthesis by human DNA polymerase kappa on a DNA template containing a single stereoisomer of dG-(+)- or dG-(−)-anti-N(2)-BPDE (7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene)". Biochemistry 41 (19): 6100–6. doi:10.1021/bi020049c. PMID 11994005.
Fischhaber PL, Gerlach VL, Feaver WJ et al. (2002). "Human DNA polymerase kappa bypasses and extends beyond thymine glycols during translesion synthesis in vitro, preferentially incorporating correct nucleotides". J. Biol. Chem. 277 (40): 37604–11. doi:10.1074/jbc.M206027200. PMID 12145297.
Bergoglio V, Bavoux C, Verbiest V et al. (2003). "Localisation of human DNA polymerase kappa to replication foci". J. Cell. Sci. 115 (Pt 23): 4413–8. doi:10.1242/jcs.00162. PMID 12414988.
Haracska L, Prakash L, Prakash S (2003). "Role of human DNA polymerase κ as an extender in translesion synthesis". Proc. Natl. Acad. Sci. U.S.A. 99 (25): 16000–5. doi:10.1073/pnas.252524999. PMC 138554. PMID 12444249.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Wolfle WT, Washington MT, Prakash L, Prakash S (2003). "Human DNA polymerase κ uses template–primer misalignment as a novel means for extending mispaired termini and for generating single-base deletions". Genes Dev. 17 (17): 2191–9. doi:10.1101/gad.1108603. PMC 196459. PMID 12952891.
Haracska L, Prakash L, Prakash S (2003). "A mechanism for the exclusion of low-fidelity human Y-family DNA polymerases from base excision repair". Genes Dev. 17 (22): 2777–85. doi:10.1101/gad.1146103. PMC 280626. PMID 14630940.
Ota T, Suzuki Y, Nishikawa T et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Suzuki N, Itoh S, Poon K et al. (2004). "Translesion synthesis past estrogen-derived DNA adducts by human DNA polymerases eta and kappa". Biochemistry 43 (20): 6304–11. doi:10.1021/bi0360298. PMID 15147214.
Ohashi E, Murakumo Y, Kanjo N et al. (2005). "Interaction of hREV1 with three human Y-family DNA polymerases". Genes Cells 9 (6): 523–31. doi:10.1111/j.1356-9597.2004.00747.x. PMID 15189446.
Washington MT, Minko IG, Johnson RE et al. (2004). "Efficient and Error-Free Replication Past a Minor-Groove DNA Adduct by the Sequential Action of Human DNA Polymerases ι and κ". Mol. Cell. Biol. 24 (13): 5687–93. doi:10.1128/MCB.24.13.5687-5693.2004. PMC 480884. PMID 15199127.
Uljon SN, Johnson RE, Edwards TA et al. (2005). "Crystal structure of the catalytic core of human DNA polymerase kappa". Structure 12 (8): 1395–404. doi:10.1016/j.str.2004.05.011. PMID 15296733.

PDB gallery

1t94: Crystal structure of the catalytic core of human DNA polymerase kappa

2oh2: Ternary Complex of Human DNA Polymerase

POLK

References

Further reading