PLCG1

Phospholipase C, gamma 1

PDB rendering based on 1hsq.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
SymbolsPLCG1 ; NCKAP3; PLC-II; PLC1; PLC148; PLCgamma1
External IDsOMIM: 172420 MGI: 97615 HomoloGene: 1997 IUPHAR: 1407 ChEMBL: 3964 GeneCards: PLCG1 Gene
EC number3.1.4.11
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez533518803
EnsemblENSG00000124181ENSMUSG00000016933
UniProtP19174Q62077
RefSeq (mRNA)NM_002660NM_021280
RefSeq (protein)NP_002651NP_067255
Location (UCSC)Chr 20:
39.77 – 39.81 Mb		Chr 2:
160.73 – 160.78 Mb
PubMed search

Phospholipase C, gamma 1, also known as PLCG1, is a protein that in humans is encoded by the PLCG1 gene.[1][2]

Function

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RASGRP1 to translocate to the Golgi apparatus, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. The receptor protein tyrosine phosphatase PTPmu (PTPRM) is capable of dephosphorylating PLCG1.[3] Two transcript variants encoding different isoforms have been found for this gene.[4]

Clinical significance

Researchers studying PLCg1 and its role in breast cancer metastasis discovered this gene can promote cancer metastasis and subsequently blocking it stopped cancer from spreading. Research is ongoing but this gene could lead to the development of new anti-cancer drugs.[5][6]

Interactions

PLCG1 has been shown to interact with:

See also


References

  1. ↑ Bristol A, Hall SM, Kriz RW, Stahl ML, Fan YS, Byers MG et al. (1988). "Phospholipase C-148: chromosomal location and deletion mapping of functional domains". Cold Spring Harb. Symp. Quant. Biol. 53 Pt 2: 915–20. PMID 3254788.
  2. ↑ Burgess WH, Dionne CA, Kaplow J, Mudd R, Friesel R, Zilberstein A et al. (September 1990). "Characterization and cDNA cloning of phospholipase C-gamma, a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase". Mol. Cell. Biol. 10 (9): 4770–7. PMC 361079. PMID 2167438.
  3. ↑ Phillips-Mason PJ, Kaur H, Burden-Gulley SM, Craig SE, Brady-Kalnay SM (2011). "Identification of phospholipase C gamma1 as a protein tyrosine phosphatase mu substrate that regulates cell migration.". J Cell Biochem 112 (1): 39–48. doi:10.1002/jcb.22710. PMC 3031780. PMID 20506511.
  4. ↑ "Entrez Gene: PLCG1 phospholipase C, gamma 1".
  5. ↑ Smith R (2008-12-14). "Scientists may be able to stop cancer spreading round the body". Health News. Telegraph.co.uk. Retrieved 2008-12-17.
  6. ↑ Sala G, Dituri F, Raimondi C, Previdi S, Maffucci T, Mazzoletti M et al. (December 2008). "Phospholipase C{gamma}1 Is Required for Metastasis Development and Progression". Cancer Res. 68 (24): 10187–96. doi:10.1158/0008-5472.CAN-08-1181. PMID 19074886.
  7. ↑ Doong H, Price J, Kim YS, Gasbarre C, Probst J, Liotta LA et al. "CAIR-1/BAG-3 forms an EGF-regulated ternary complex with phospholipase C-gamma and Hsp70/Hsc70". Oncogene 19 (38): 4385–95. doi:10.1038/sj.onc.1203797. PMID 10980614.
  8. ↑ van Dijk TB, van Den Akker E, Amelsvoort MP, Mano H, Löwenberg B, von Lindern M. "Stem cell factor induces phosphatidylinositol 3'-kinase-dependent Lyn/Tec/Dok-1 complex formation in hematopoietic cells". Blood 96 (10): 3406–13. PMID 11071635.
  9. ↑ Jhun BH, Rivnay B, Price D, Avraham H. "The MATK tyrosine kinase interacts in a specific and SH2-dependent manner with c-Kit". J. Biol. Chem. 270 (16): 9661–6. doi:10.1074/jbc.270.16.9661. PMID 7536744.
  10. ↑ Pumphrey NJ, Taylor V, Freeman S, Douglas MR, Bradfield PF, Young SP et al. "Differential association of cytoplasmic signalling molecules SHP-1, SHP-2, SHIP and phospholipase C-gamma1 with PECAM-1/CD31". FEBS Lett. 450 (1-2): 77–83. doi:10.1016/s0014-5793(99)00446-9. PMID 10350061.
  11. ↑ 11.0 11.1 Tvorogov D, Carpenter G. "EGF-dependent association of phospholipase C-gamma1 with c-Cbl". Exp. Cell Res. 277 (1): 86–94. doi:10.1006/excr.2002.5545. PMID 12061819.
  12. ↑ Graham LJ, Stoica BA, Shapiro M, DeBell KE, Rellahan B, Laborda J et al. "Sequences surrounding the Src-homology 3 domain of phospholipase Cgamma-1 increase the domain's association with Cbl". Biochem. Biophys. Res. Commun. 249 (2): 537–41. doi:10.1006/bbrc.1998.9177. PMID 9712732.
  13. ↑ Bedrin MS, Abolafia CM, Thompson JF. "Cytoskeletal association of epidermal growth factor receptor and associated signaling proteins is regulated by cell density in IEC-6 intestinal cells". J. Cell. Physiol. 172 (1): 126–36. doi:10.1002/(SICI)1097-4652(199707)172:1<126::AID-JCP14>3.0.CO;2-A. PMID 9207933.
  14. ↑ Chang JS, Seok H, Kwon TK, Min DS, Ahn BH, Lee YH et al. "Interaction of elongation factor-1alpha and pleckstrin homology domain of phospholipase C-gamma 1 with activating its activity". J. Biol. Chem. 277 (22): 19697–702. doi:10.1074/jbc.M111206200. PMID 11886851.
  15. ↑ Cunningham SA, Arrate MP, Brock TA, Waxham MN. "Interactions of FLT-1 and KDR with phospholipase C gamma: identification of the phosphotyrosine binding sites". Biochem. Biophys. Res. Commun. 240 (3): 635–9. doi:10.1006/bbrc.1997.7719. PMID 9398617.
  16. ↑ Ueno E, Haruta T, Uno T, Usui I, Iwata M, Takano A et al. "Potential role of Gab1 and phospholipase C-gamma in osmotic shock-induced glucose uptake in 3T3-L1 adipocytes". Horm. Metab. Res. 33 (7): 402–6. doi:10.1055/s-2001-16227. PMID 11507676.
  17. ↑ Holgado-Madruga M, Emlet DR, Moscatello DK, Godwin AK, Wong AJ. "A Grb2-associated docking protein in EGF- and insulin-receptor signalling". Nature 379 (6565): 560–4. doi:10.1038/379560a0. PMID 8596638.
  18. ↑ Haendeler J, Yin G, Hojo Y, Saito Y, Melaragno M, Yan C et al. "GIT1 mediates Src-dependent activation of phospholipase Cgamma by angiotensin II and epidermal growth factor". J. Biol. Chem. 278 (50): 49936–44. doi:10.1074/jbc.M307317200. PMID 14523024.
  19. ↑ Pei Z, Maloney JA, Yang L, Williamson JR. "A new function for phospholipase C-gamma1: coupling to the adaptor protein GRB2". Arch. Biochem. Biophys. 345 (1): 103–10. doi:10.1006/abbi.1997.0245. PMID 9281317.
  20. ↑ Nel AE, Gupta S, Lee L, Ledbetter JA, Kanner SB. "Ligation of the T-cell antigen receptor (TCR) induces association of hSos1, ZAP-70, phospholipase C-gamma 1, and other phosphoproteins with Grb2 and the zeta-chain of the TCR". J. Biol. Chem. 270 (31): 18428–36. doi:10.1074/jbc.270.31.18428. PMID 7629168.
  21. ↑ 21.0 21.1 Scholler JK, Perez-Villar JJ, O'Day K, Kanner SB. "Engagement of the T lymphocyte antigen receptor regulates association of son-of-sevenless homologues with the SH3 domain of phospholipase Cgamma1". Eur. J. Immunol. 30 (8): 2378–87. doi:10.1002/1521-4141(2000)30:8<2378::AID-IMMU2378>3.0.CO;2-E. PMID 10940929.
  22. ↑ Peles E, Levy RB, Or E, Ullrich A, Yarden Y. "Oncogenic forms of the neu/HER2 tyrosine kinase are permanently coupled to phospholipase C gamma". EMBO J. 10 (8): 2077–86. PMC 452891. PMID 1676673.
  23. ↑ Arteaga CL, Johnson MD, Todderud G, Coffey RJ, Carpenter G, Page DL. "Elevated content of the tyrosine kinase substrate phospholipase C-gamma 1 in primary human breast carcinomas". Proc. Natl. Acad. Sci. U.S.A. 88 (23): 10435–9. doi:10.1073/pnas.88.23.10435. PMC 52943. PMID 1683701.
  24. ↑ Sozzani P, Hasan L, Séguélas MH, Caput D, Ferrara P, Pipy B et al. "IL-13 induces tyrosine phosphorylation of phospholipase C gamma-1 following IRS-2 association in human monocytes: relationship with the inhibitory effect of IL-13 on ROI production". Biochem. Biophys. Res. Commun. 244 (3): 665–70. doi:10.1006/bbrc.1998.8314. PMID 9535722.
  25. ↑ Perez-Villar JJ, Kanner SB. "Regulated association between the tyrosine kinase Emt/Itk/Tsk and phospholipase-C gamma 1 in human T lymphocytes". J. Immunol. 163 (12): 6435–41. PMID 10586033.
  26. ↑ Hao S, August A. "The proline rich region of the Tec homology domain of ITK regulates its activity". FEBS Lett. 525 (1-3): 53–8. doi:10.1016/s0014-5793(02)03066-1. PMID 12163161.
  27. ↑ Oneyama C, Nakano H, Sharma SV. "UCS15A, a novel small molecule, SH3 domain-mediated protein-protein interaction blocking drug". Oncogene 21 (13): 2037–50. doi:10.1038/sj.onc.1205271. PMID 11960376.
  28. ↑ Jabado N, Jauliac S, Pallier A, Bernard F, Fischer A, Hivroz C. "Sam68 association with p120GAP in CD4+ T cells is dependent on CD4 molecule expression". J. Immunol. 161 (6): 2798–803. PMID 9743338.
  29. ↑ Shen Z, Batzer A, Koehler JA, Polakis P, Schlessinger J, Lydon NB et al. "Evidence for SH3 domain directed binding and phosphorylation of Sam68 by Src". Oncogene 18 (33): 4647–53. doi:10.1038/sj.onc.1203079. PMID 10467411.
  30. ↑ Zhang W, Trible RP, Samelson LE. "LAT palmitoylation: its essential role in membrane microdomain targeting and tyrosine phosphorylation during T cell activation". Immunity 9 (2): 239–46. doi:10.1016/s1074-7613(00)80606-8. PMID 9729044.
  31. ↑ Paz PE, Wang S, Clarke H, Lu X, Stokoe D, Abo A. "Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells". Biochem. J. 356 (Pt 2): 461–71. doi:10.1042/0264-6021:3560461. PMC 1221857. PMID 11368773.
  32. ↑ Zhang W, Sloan-Lancaster J, Kitchen J, Trible RP, Samelson LE. "LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation". Cell 92 (1): 83–92. doi:10.1016/S0092-8674(00)80901-0. PMID 9489702.
  33. ↑ Yablonski D, Kadlecek T, Weiss A. "Identification of a phospholipase C-gamma1 (PLC-gamma1) SH3 domain-binding site in SLP-76 required for T-cell receptor-mediated activation of PLC-gamma1 and NFAT". Mol. Cell. Biol. 21 (13): 4208–18. doi:10.1128/MCB.21.13.4208-4218.2001. PMC 87082. PMID 11390650.
  34. ↑ Eriksson A, Nånberg E, Rönnstrand L, Engström U, Hellman U, Rupp E et al. "Demonstration of functionally different interactions between phospholipase C-gamma and the two types of platelet-derived growth factor receptors". J. Biol. Chem. 270 (13): 7773–81. doi:10.1074/jbc.270.13.7773. PMID 7535778.
  35. ↑ Jang IH, Lee S, Park JB, Kim JH, Lee CS, Hur EM et al. "The direct interaction of phospholipase C-gamma 1 with phospholipase D2 is important for epidermal growth factor signaling". J. Biol. Chem. 278 (20): 18184–90. doi:10.1074/jbc.M208438200. PMID 12646582.
  36. ↑ Thodeti CK, Massoumi R, Bindslev L, Sjölander A. "Leukotriene D4 induces association of active RhoA with phospholipase C-gamma1 in intestinal epithelial cells". Biochem. J. 365 (Pt 1): 157–63. doi:10.1042/BJ20020248. PMC 1222665. PMID 12071848.
  37. ↑ Kim MJ, Chang JS, Park SK, Hwang JI, Ryu SH, Suh PG. "Direct interaction of SOS1 Ras exchange protein with the SH3 domain of phospholipase C-gamma1". Biochemistry 39 (29): 8674–82. doi:10.1021/bi992558t. PMID 10913276.
  38. ↑ Kapeller R, Moriarty A, Strauss A, Stubdal H, Theriault K, Siebert E et al. "Tyrosine phosphorylation of tub and its association with Src homology 2 domain-containing proteins implicate tub in intracellular signaling by insulin". J. Biol. Chem. 274 (35): 24980–6. doi:10.1074/jbc.274.35.24980. PMID 10455176.
  39. ↑ Ohmichi M, Decker SJ, Pang L, Saltiel AR. "Nerve growth factor binds to the 140 kd trk proto-oncogene product and stimulates its association with the src homology domain of phospholipase C gamma 1". Biochem. Biophys. Res. Commun. 179 (1): 217–23. doi:10.1016/0006-291x(91)91357-i. PMID 1715690.
  40. ↑ Qian X, Riccio A, Zhang Y, Ginty DD. "Identification and characterization of novel substrates of Trk receptors in developing neurons". Neuron 21 (5): 1017–29. doi:10.1016/s0896-6273(00)80620-0. PMID 9856458.
  41. ↑ 41.0 41.1 Meakin SO, MacDonald JI, Gryz EA, Kubu CJ, Verdi JM. "The signaling adapter FRS-2 competes with Shc for binding to the nerve growth factor receptor TrkA. A model for discriminating proliferation and differentiation". J. Biol. Chem. 274 (14): 9861–70. doi:10.1074/jbc.274.14.9861. PMID 10092678.
  42. ↑ Koch A, Mancini A, Stefan M, Niedenthal R, Niemann H, Tamura T. "Direct interaction of nerve growth factor receptor, TrkA, with non-receptor tyrosine kinase, c-Abl, through the activation loop". FEBS Lett. 469 (1): 72–6. doi:10.1016/s0014-5793(00)01242-4. PMID 10708759.
  43. ↑ Suzuki S, Mizutani M, Suzuki K, Yamada M, Kojima M, Hatanaka H et al. "Brain-derived neurotrophic factor promotes interaction of the Nck2 adaptor protein with the TrkB tyrosine kinase receptor". Biochem. Biophys. Res. Commun. 294 (5): 1087–92. doi:10.1016/S0006-291X(02)00606-X. PMID 12074588.
  44. ↑ Bertagnolo V, Marchisio M, Volinia S, Caramelli E, Capitani S. "Nuclear association of tyrosine-phosphorylated Vav to phospholipase C-gamma1 and phosphoinositide 3-kinase during granulocytic differentiation of HL-60 cells". FEBS Lett. 441 (3): 480–4. doi:10.1016/s0014-5793(98)01593-2. PMID 9891995.
  45. ↑ Banin S, Truong O, Katz DR, Waterfield MD, Brickell PM, Gout I. "Wiskott-Aldrich syndrome protein (WASp) is a binding partner for c-Src family protein-tyrosine kinases". Curr. Biol. 6 (8): 981–8. doi:10.1016/s0960-9822(02)00642-5. PMID 8805332.
  46. ↑ Finan PM, Soames CJ, Wilson L, Nelson DL, Stewart DM, Truong O et al. "Identification of regions of the Wiskott-Aldrich syndrome protein responsible for association with selected Src homology 3 domains". J. Biol. Chem. 271 (42): 26291–5. doi:10.1074/jbc.271.42.26291. PMID 8824280.