Optimal cutting temperature compound
Frozen section procedure: Tissue embedded within OCT, mounted on a chuck in a cryostat and ready for section production
Optimal Cutting Temperature compound (OCT) is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a sample onto slides for analysis.
Components
Composition/Data on Components: As stated on the bottle: "10.24% polyvinyl alcohol 4.26% polyethylene glycol 85.5% non-reactive ingredients"[1]
See also
References
- ↑ "OCT Compound, 27050, MSDS". Tedpella.com. Retrieved 2011-08-19.
Further reading
- Turbett, Gavin; Sellner, Loryn (1997). "The use of optimal cutting temperature compound can inhibit amplification by polymerase chain reaction". Diagnostic Molecular Pathology 6 (5): 298–303. doi:10.1097/00019606-199710000-00009. PMID 9458390.