Optical projection tomography
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Optical projection tomography is a form of tomography involving optical microscopy.[1]
It is in many ways the optical equivalent of X-ray computed tomography or the medical CT scan. OPT differs in the way that it assumes parallel ray projection as opposed to fan beam projection as is the case for X-ray CT.
The technique has already contributed to a large number of studies aimed at addressing a broad range of biological questions in diverse systems such as human, mice, chicken, fly, zebrafish, and plants. More recent adaptations have further enabled the use of the technique for studies of specimens on the adult mouse organ scale,[2] individual cell nuclei, and longitudinal assessments of organ cultures.
Fluorescence optical projection tomography images the distribution of dyes in the specimen.
See also
References
- ↑ "Optical projection tomography as a tool for 3D microscopy and gene expression studies". Science 296 (5567): 541–5. April 2002. doi:10.1126/science.1068206. PMID 11964482.
- ↑ "Image Processing Assisted Algorithms for Optical Projection Tomography". IEEE Transactions on Medical Imaging 31 (1): 1–15. January 2012. doi:10.1109/TMI.2011.2161590. PMID 21768046.
External links
- http://genex.hgu.mrc.ac.uk/OPT_Microscopy/optwebsite/frontpage/index.htm
- Video I: Optical Projection Tomography of a mouse left lateral liver lobe.
- Video II: Optical Projection Tomography of an embryonic stomach, intestine and pancreas of a mouse.
- Inner World of carnivorous plants from the John Innes Centre