Nanopore

A nanopore is a very small hole. It may, for example, be created by a pore-forming protein or as a hole in synthetic materials such as silicon or graphene.

When a nanopore is present in an electrically insulating membrane, it can be used as a single-molecule detector. It can be a biological protein channel in a high electrical resistance lipid bilayer, a pore in a solid-state membrane or a hybrid of these - a protein channel set in a synthetic membrane. The detection principle is based on monitoring the ionic current passing through the nanopore as a voltage is applied across the membrane. When the nanopore is of molecular dimensions, passage of molecules (e.g., DNA) cause interruptions of the "open" current level, leading to a "translocation event" signal. The passage of RNA or single-stranded DNA molecules through the membrane-embedded alpha-hemolysin channel (1.5 nm diameter), for example, causes a ~90% blockage of the current (measured at 1 M KCl solution).^[1]

It may be considered a Coulter counter for much smaller particles.

Biological/Protein Nanopores

Nanopores may be formed by pore-forming proteins,^[2] typically a hollow core passing through a mushroom-shaped protein molecule. Examples of pore-forming proteins are alpha hemolysin and MspA porin. In typical laboratory nanopore experiments, a single protein nanopore is inserted into a lipid bilayer membrane and single-channel electrophysiology measurements are taken.

Solid State Nanopores

Solid-state nanopores are generally made in silicon compound membranes, one of the most common being silicon nitride. Solid-state nanopores can be manufactured with several techniques including ion-beam sculpting^[3] and electron beams.^[4]

More recently, the use of graphene^[5] as a material for solid-state nanopore sensing has been explored.

Nanopore measurement in track etched membrane

Since the discovery of track-etched technology in the late 1960s, filter membranes with needed diameter have found application potential in various fields including food safety, Environmental pollution, biology, medicine, fuel cell, and chemistry.These track-etched membranes are typically made in polymer membrane through track-etching procedure, during which the polymer membrane is first irradiated by heavy ion beam to form tracks and then cylindrical pores or asymmetric pores are created along the track after wet etching.

As important as fabrication of the filter membranes with proper diameters, characterizations and measurements of these materials are of the same paramount. Until now, a few of methods have been developed, which can be classified into the following categories according to the physical mechanisms they exploited: Imaging methods such as Scanning Electron Microscopy (SEM),Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM); Fluid transports such as Bubble Point11 and Gas Transport; Fluid adsorptions such as Nitrogen Adsorption/Desorption (BEH), Mercury Porosimetry, Liquid-Vapor Equilibrium (BJH), Gas-Liquid Equilibrium (Permoporometry) and Liquid-Solid Equilibrium (Thermoporometry); Electronic Conductance; Ultrasonic Spectroscopy;19 Molecular Transport.

More recently, the use of light transmission technique ^[6] as a method for nanopore size measurement has been proposed.

Ion current rectification for analytical chemistry

Ion current rectification is a very important phenomenon for nanopore. Siwy research group has contribute significantly to understand this experimentally and theoretically. Jiang lei group has been pioneering in constructing smart switching nanopore via incorporating intelligent polymer or molecules into the nanopore.

Ion current rectification can als be used for drug sensor ^[7][8] and be employed to investigate charge status in the polymer membrane.^[9]

 More information can  be found in webpage ( http://nanopore.weebly.com/ )

Nanopore Based Sequencing

The observation that a passing strand of DNA containing different bases results in different blocking levels has led to the nanopore sequencing hypothesis. Oxford Nanopore Technologies and Professor Hagan Bayley's laboratories have shown identification of individual nucleotides including methylated cytosine as they pass through a modified hemolysin nanopore.^[10]

Such sequencing, if successful, could revolutionize the field of genomics, as sequencing would be simplified and have the potential for dramatic improvements in power and cost over current versions that use fluorescence/luminescence and optical instrumentation to detect this photon signal. Apart from rapid DNA sequencing, other applications include separation of single stranded and double stranded DNA in solution, and the determination of length of polymers. At this stage, nanopores are making contributions to the understanding of polymer biophysics, as well as to single-molecule analysis of DNA-protein interactions.

Size Tunable Nanopores

Size-tunable elastomeric nanopores have been fabricated, allowing accurate measurement of nanoparticles as they occlude the flow of ionic current.This measurement methodology can be used to measure a wide range of particle types. In contrast to the limitations of solid-state pores, they allow for the optimisation of the resistance pulse magnitude relative to the background current by matching the pore-size closely to the particle-size. As detection occurs on a particle by particle basis, the true average and polydispersity distribution can be determined.^[11][12] Using this principle, the world's only commercial tunable nanopore-based particle detection system has been developed by Izon Science Ltd.

Alternate Definition

These can be about 20 nm in a diameter. They are integrated into artificially constructed encapsulated cells of silicon wafers. These pores allow small molecules like oxygen, glucose and insulin to pass however they prevent large immune system molecules like immunoglobins from passing. This way rat pancreatic cells are microencapsulated, they receive nutrients and release insulin through nanopores being totally isolated from their neighboring environment i.e. foreign cells. This knowledge can help to replace nonfunctional islets of Langerhans cells in the pancreas (responsible for producing insulin), by harvested piglet cells. They can be implanted underneath the human skin without the need of immunosuppressants which put diabetic patients at a risk of infection.

See also

• Nanopore sequencing
• Nanofluidics

References

• Xu Hou, Wei Guo, Lei Jiang (2011). "Biomimetic Smart Nanopores and Nanochannels". Chem. Soc. Rev. 40 (5): 2041–3052. doi:10.1039/C0CS00053A. 

• Xu Hou, Lei Jiang (2009). "Learning from Nature: Building Bio-Inspired Smart Nanochannels". ACS Nano 3 (11): 3339–42. doi:10.1021/nn901402b. PMID 19928930. 

• Xu Hou, Huacheng Zhang, Lei Jiang (2012). "Building Bio-Inspired Artificial Functional Nanochannels: From Symmetric to Asymmetric Modification". Angewandte Chemie International Edition 51 (22): 5296–5307. doi:10.1002/anie.201104904. 

External links and references

• Nanopore Research in China
• Nanopore in China
• Nanopore in Changchun
• TheNanoporeSite Recent nanopore papers, meetings and research groups
• Links to nanopore research groups
• The Harvard Nanopore Group
• NanoSEQ — The Global Exchange for Nanopore Research
• Oxford Nanopore Technologies
• Nabsys
• Sequenom
• UC Santa Cruz Nanopore Project
• Wang H, Dunning JE, Huang AP, Nyamwanda JA, Branton D (September 2004). "DNA heterogeneity and phosphorylation unveiled by single-molecule electrophoresis". Proc. Natl. Acad. Sci. U.S.A. 101 (37): 13472–7. doi:10.1073/pnas.0405568101. PMC 518781. PMID 15342914. 
• Computer simulations of nanopore devices
• Conical Nanopore Sensors
• Biomimetic Channels and Ionic Devices