NDC80

NDC80 kinetochore complex component

PDB rendering based on 2igp.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
2IGP, 2VE7, 3IZ0

Identifiers

Symbols

NDC80 ; HEC; HEC1; HsHec1; KNTC2; TID3; hsNDC80

External IDs

OMIM: 607272 MGI: 1914302 HomoloGene: 38141 ChEMBL: 5660 GeneCards: NDC80 Gene

Gene ontology
Molecular function	• protein binding
Cellular component	• chromosome, centromeric region • kinetochore • condensed chromosome kinetochore • condensed nuclear chromosome outer kinetochore • nucleus • cytosol • membrane • Ndc80 complex
Biological process	• mitotic sister chromatid segregation • establishment of mitotic spindle orientation • mitotic cell cycle • cell cycle • spindle organization • mitotic spindle organization • chromosome segregation • mitotic nuclear division • attachment of spindle microtubules to kinetochore • phosphatidylinositol-mediated signaling
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 18:
2.57 – 2.62 Mb

Chr 17:
71.5 – 71.53 Mb

PubMed search

Kinetochore protein NDC80 homolog is a protein that in humans is encoded by the NDC80 gene.^[1]^[2]^[3]

HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]^[3]

Interactions

NDC80 has been shown to interact with MIS12,^[4]^[5] NEK2^[6]^[7] and PSMC2.^[7]

References

↑ Chen Y, Riley DJ, Chen PL, Lee WH (Oct 1997). "HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis". Mol Cell Biol 17 (10): 6049–56. PMC 232454. PMID 9315664.
↑ Martin-Lluesma S, Stucke VM, Nigg EA (Sep 2002). "Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2". Science 297 (5590): 2267–70. doi:10.1126/science.1075596. PMID 12351790.
↑ 3.0 3.1 "Entrez Gene: NDC80 NDC80 homolog, kinetochore complex component (S. cerevisiae)".
↑ Cheeseman, Iain M; Niessen Sherry; Anderson Scott; Hyndman Francie; Yates John R; Oegema Karen; Desai Arshad (Sep 2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension". Genes Dev. (United States) 18 (18): 2255–68. doi:10.1101/gad.1234104. ISSN 0890-9369. PMC 517519. PMID 15371340.
↑ Obuse, Chikashi; Iwasaki Osamu; Kiyomitsu Tomomi; Goshima Gohta; Toyoda Yusuke; Yanagida Mitsuhiro (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nat. Cell Biol. (England) 6 (11): 1135–41. doi:10.1038/ncb1187. ISSN 1465-7392. PMID 15502821.
↑ Chen, Yumay; Riley Daniel J; Zheng Lei; Chen Phang-Lang; Lee Wen-Hwa (Dec 2002). "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". J. Biol. Chem. (United States) 277 (51): 49408–16. doi:10.1074/jbc.M207069200. ISSN 0021-9258. PMID 12386167.
↑ 7.0 7.1 Chen, Y; Sharp Z D; Lee W H (Sep 1997). "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins". J. Biol. Chem. (UNITED STATES) 272 (38): 24081–7. doi:10.1074/jbc.272.38.24081. ISSN 0021-9258. PMID 9295362.

Chen Y, Sharp ZD, Lee WH (1997). "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins". J. Biol. Chem. 272 (38): 24081–7. doi:10.1074/jbc.272.38.24081. PMID 9295362.
Zheng L, Chen Y, Lee WH (1999). "Hec1p, an Evolutionarily Conserved Coiled-Coil Protein, Modulates Chromosome Segregation through Interaction with SMC Proteins". Mol. Cell. Biol. 19 (8): 5417–28. PMC 84384. PMID 10409732.
Zheng L; Chen Y; Riley DJ et al. (2000). "Retinoblastoma Protein Enhances the Fidelity of Chromosome Segregation Mediated by hsHec1p". Mol. Cell. Biol. 20 (10): 3529–37. doi:10.1128/MCB.20.10.3529-3537.2000. PMC 85645. PMID 10779342.
Chen Y; Riley DJ; Zheng L et al. (2003). "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". J. Biol. Chem. 277 (51): 49408–16. doi:10.1074/jbc.M207069200. PMID 12386167.
Strausberg RL; Feingold EA; Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Tien AC; Lin MH; Su LJ et al. (2004). "Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model". Mol. Cell Proteomics 3 (1): 93–104. doi:10.1074/mcp.M300072-MCP200. PMID 14602875.
DeLuca JG; Howell BJ; Canman JC et al. (2003). "Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores". Curr. Biol. 13 (23): 2103–9. doi:10.1016/j.cub.2003.10.056. PMID 14654001.
Bharadwaj R, Qi W, Yu H (2004). "Identification of two novel components of the human NDC80 kinetochore complex". J. Biol. Chem. 279 (13): 13076–85. doi:10.1074/jbc.M310224200. PMID 14699129.
Lou Y; Yao J; Zereshki A et al. (2004). "NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling". J. Biol. Chem. 279 (19): 20049–57. doi:10.1074/jbc.M314205200. PMID 14978040.
Joseph J; Liu ST; Jablonski SA et al. (2004). "The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo". Curr. Biol. 14 (7): 611–7. doi:10.1016/j.cub.2004.03.031. PMID 15062103.
Steensgaard P; Garrè M; Muradore I et al. (2005). "Sgt1 is required for human kinetochore assembly". EMBO Rep. 5 (6): 626–31. doi:10.1038/sj.embor.7400154. PMC 1299074. PMID 15133482.
Stucke VM, Baumann C, Nigg EA (2005). "Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1". Chromosoma 113 (1): 1–15. doi:10.1007/s00412-004-0288-2. PMID 15235793.
Meraldi P, Draviam VM, Sorger PK (2004). "Timing and checkpoints in the regulation of mitotic progression". Dev. Cell 7 (1): 45–60. doi:10.1016/j.devcel.2004.06.006. PMID 15239953.
Cheeseman IM; Niessen S; Anderson S et al. (2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension". Genes Dev. 18 (18): 2255–68. doi:10.1101/gad.1234104. PMC 517519. PMID 15371340.
Gerhard DS; Wagner L; Feingold EA et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Obuse C; Iwasaki O; Kiyomitsu T et al. (2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nat. Cell Biol. 6 (11): 1135–41. doi:10.1038/ncb1187. PMID 15502821.
Le XF; Lammayot A; Gold D et al. (2005). "Genes affecting the cell cycle, growth, maintenance, and drug sensitivity are preferentially regulated by anti-HER2 antibody through phosphatidylinositol 3-kinase-AKT signaling". J. Biol. Chem. 280 (3): 2092–104. doi:10.1074/jbc.M403080200. PMID 15504738.
DeLuca JG; Dong Y; Hergert P et al. (2005). "Hec1 and Nuf2 Are Core Components of the Kinetochore Outer Plate Essential for Organizing Microtubule Attachment Sites". Mol. Biol. Cell 16 (2): 519–31. doi:10.1091/mbc.E04-09-0852. PMC 545888. PMID 15548592.

PDB gallery

2igp: Crystal Structure of Hec1 CH domain

NDC80

Interactions

References

Further reading