mir-160 microRNA precursor family

mir-160 microRNA precursor family
Predicted secondary structure and sequence conservation of mir-160
Identifiers
Symbol mir-160
Rfam RF00247
miRBase MI0000190
miRBase family MIPF0000032
Other data
RNA type Gene; miRNA
Domain(s) Eukaryota
GO 0035195 0035068
SO 0001244

In molecular biology, mir-160 is a microRNA that has been predicted or experimentally confirmed in a range of plant species including Arabidopsis thaliana (mouse-ear cress) and Oryza sativa (rice).[1] miR-160 is predicted to bind complementary sites in the untranslated regions of auxin response factor genes to regulate their expression.[2] The hairpin precursors (represented here) are predicted based on base pairing and cross-species conservation; their extents are not known. In this case, the mature sequence is excised from the 5' arm of the hairpin.

Specifically, 3 of A. thaliana's 23 auxin-response factor genes are thought to be post-transcriptionally regulated by mir-160.[3][4] When one of these targets (ARF17) is manipulated to become miRNA-resistant, several developmental defects can be observed in the host plant.[3] This experiment has been repeated with another mir-160 target, ARF10, and results highlighted a regulatory role in post-embryonic development and seed germination.[5]

References

  1. ↑ "miRNA gene family: MIR160". mirBASE. University of Manchester. Retrieved 5 September 2011.
  2. ↑ Rhoades, MW; Reinhart BJ; Lim LP; Burge CB; Bartel B; Bartel DP (2002). "Prediction of plant microRNA targets". Cell 110 (4): 513–520. doi:10.1016/S0092-8674(02)00863-2. PMID 12202040.
  3. ↑ 3.0 3.1 Mallory, AC; Bartel, DP, Bartel, B (May 2005). "MicroRNA-directed regulation of Arabidopsis AUXIN RESPONSE FACTOR17 is essential for proper development and modulates expression of early auxin response genes.". The Plant cell 17 (5): 1360–75. doi:10.1105/tpc.105.031716. PMC 1091760. PMID 15829600.
  4. ↑ Liu, X; Huang, J; Wang, Y; Khanna, K; Xie, Z; Owen, HA; Zhao, D (May 2010). "The role of floral organs in carpels, an Arabidopsis loss-of-function mutation in MicroRNA160a, in organogenesis and the mechanism regulating its expression.". The Plant journal : for cell and molecular biology 62 (3): 416–28. doi:10.1111/j.1365-313X.2010.04164.x. PMID 20136729.
  5. ↑ Liu, PP; Montgomery, TA, Fahlgren, N, Kasschau, KD, Nonogaki, H, Carrington, JC (October 2007). "Repression of AUXIN RESPONSE FACTOR10 by microRNA160 is critical for seed germination and post-germination stages.". The Plant journal : for cell and molecular biology 52 (1): 133–46. doi:10.1111/j.1365-313X.2007.03218.x. PMID 17672844.

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