MCM3
DNA replication licensing factor MCM3 is a protein that in humans is encoded by the MCM3 gene.[1]
Function
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.[2]
Interactions
MCM3 has been shown to interact with:
- CDC45-related protein,[3]
- CDC6,[4][5]
- DBF4,[3]
- MCM2[3]
- MCM3AP,[6][7][8]
- MCM5,[3][9]
- MCM7,[3][4][10][11]
- ORC4L,[3] and
- ORC5L.[3]
See also
- Mini Chromosome Maintenance
References
- ↑ Thömmes P, Fett R, Schray B, Burkhart R, Barnes M, Kennedy C et al. (April 1992). "Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein". Nucleic Acids Res 20 (5): 1069–74. doi:10.1093/nar/20.5.1069. PMC 312092. PMID 1549468. Vancouver style error (help)
- ↑ "Entrez Gene: MCM3 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)".
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 Kneissl M, Pütter V, Szalay AA, Grummt F (March 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612. Vancouver style error (help)
- ↑ 4.0 4.1 Fujita M, Yamada C, Goto H, Yokoyama N, Kuzushima K, Inagaki M et al. (September 1999). "Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation". J. Biol. Chem. 274 (36): 25927–32. doi:10.1074/jbc.274.36.25927. PMID 10464337.
- ↑ Méndez J, Stillman B (November 2000). "Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis". Mol. Cell. Biol. 20 (22): 8602–12. doi:10.1128/mcb.20.22.8602-8612.2000. PMC 102165. PMID 11046155. Vancouver style error (help)
- ↑ Takei Y, Assenberg M, Tsujimoto G, Laskey R (November 2002). "The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with MCM3". J. Biol. Chem. 277 (45): 43121–5. doi:10.1074/jbc.C200442200. PMID 12226073.
- ↑ Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E et al. (April 2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood 95 (7): 2321–8. PMID 10733502.
- ↑ Takei Y, Tsujimoto G (August 1998). "Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization". J. Biol. Chem. 273 (35): 22177–80. doi:10.1074/jbc.273.35.22177. PMID 9712829.
- ↑ DaFonseca CJ, Shu F, Zhang JJ (March 2001). "Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma". Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3034–9. doi:10.1073/pnas.061487598. PMC 30602. PMID 11248027.
- ↑ Fujita M, Kiyono T, Hayashi Y, Ishibashi M (April 1997). "In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP". J. Biol. Chem. 272 (16): 10928–35. doi:10.1074/jbc.272.16.10928. PMID 9099751.
- ↑ Fujita M, Ishimi Y, Nakamura H, Kiyono T, Tsurumi T (March 2002). "Nuclear organization of DNA replication initiation proteins in mammalian cells". J. Biol. Chem. 277 (12): 10354–61. doi:10.1074/jbc.M111398200. PMID 11779870.
Further reading
- Musahl C, Schulte D, Burkhart R, Knippers R (1995). "A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins". Eur. J. Biochem. 230 (3): 1096–101. doi:10.1111/j.1432-1033.1995.tb20660.x. PMID 7601140.
- Schulte D, Burkhart R, Musahl C, Hu B, Schlatterer C, Hameister H et al. (1995). "Expression, phosphorylation and nuclear localization of the human P1 protein, a homologue of the yeast Mcm 3 replication protein". J. Cell. Sci. 108 (4): 1381–9. PMID 7615659.
- Starborg M, Brundell E, Gell K, Larsson C, White I, Daneholt B et al. (1995). "A murine replication protein accumulates temporarily in the heterochromatic regions of nuclei prior to initiation of DNA replication". J. Cell. Sci. 108 (3): 927–34. PMID 7622621.
- Burkhart R, Schulte D, Hu D, Musahl C, Göhring F, Knippers R (1995). "Interactions of human nuclear proteins P1Mcm3 and P1Cdc46". Eur. J. Biochem. 228 (2): 431–8. doi:10.1111/j.1432-1033.1995.tb20281.x. PMID 7705359. Vancouver style error (help)
- Kubota Y, Mimura S, Nishimoto S, Takisawa H, Nojima H (1995). "Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor". Cell 81 (4): 601–9. doi:10.1016/0092-8674(95)90081-0. PMID 7758114.
- Madine MA, Khoo CY, Mills AD, Laskey RA (1995). "MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells". Nature 375 (6530): 421–4. doi:10.1038/375421a0. PMID 7760938.
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Hu B, Burkhart R, Schulte D, Musahl C, Knippers R (1994). "The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins". Nucleic Acids Res. 21 (23): 5289–93. doi:10.1093/nar/21.23.5289-a. PMC 310560. PMID 8265339.
- Schulte D, Richter A, Burkhart R, Musahl C, Knippers R (1996). "Properties of the human nuclear protein p85Mcm. Expression, nuclear localization and interaction with other Mcm proteins". Eur. J. Biochem. 235 (1–2): 144–51. doi:10.1111/j.1432-1033.1996.00144.x. PMID 8631321.
- Ishimi Y, Ichinose S, Omori A, Sato K, Kimura H (1996). "Binding of human minichromosome maintenance proteins with histone H3". J. Biol. Chem. 271 (39): 24115–22. doi:10.1074/jbc.271.39.24115. PMID 8798650.
- Sato N, Arai K, Masai H (1997). "Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7". EMBO J. 16 (14): 4340–51. doi:10.1093/emboj/16.14.4340. PMC 1170060. PMID 9250678.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Takei Y, Tsujimoto G (1998). "Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization". J. Biol. Chem. 273 (35): 22177–80. doi:10.1074/jbc.273.35.22177. PMID 9712829.
- Zhang JJ, Zhao Y, Chait BT, Lathem WW, Ritzi M, Knippers R et al. (1999). "Ser727-dependent recruitment of MCM5 by Stat1alpha in IFN-gamma-induced transcriptional activation". EMBO J. 17 (23): 6963–71. doi:10.1093/emboj/17.23.6963. PMC 1171044. PMID 9843502.
- Yankulov K, Todorov I, Romanowski P, Licatalosi D, Cilli K, McCracken S et al. (1999). "MCM Proteins Are Associated with RNA Polymerase II Holoenzyme". Mol. Cell. Biol. 19 (9): 6154–63. PMC 84545. PMID 10454562.
- Fujita M, Yamada C, Goto H, Yokoyama N, Kuzushima K, Inagaki M et al. (1999). "Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation". J. Biol. Chem. 274 (36): 25927–32. doi:10.1074/jbc.274.36.25927. PMID 10464337.
- Kuwahara K, Yoshida M, Kondo E, Sakata A, Watanabe Y, Abe E et al. (2000). "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication". Blood 95 (7): 2321–8. PMID 10733502.
- Hofmann Y, Becker J, Wright F, Avner ED, Mrug M, Guay-Woodford LM et al. (2000). "Genomic structure of the gene for the human P1 protein (MCM3) and its exclusion as a candidate for autosomal recessive polycystic kidney disease". Eur. J. Hum. Genet. 8 (3): 163–6. doi:10.1038/sj.ejhg.5200426. PMID 10780780.
- Abe E, Kuwahara K, Yoshida M, Suzuki M, Terasaki H, Matsuo Y et al. (2000). "Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication". Gene 255 (2): 219–27. doi:10.1016/S0378-1119(00)00336-X. PMID 11024281.