Lys-N

Lys-N is a metalloendopeptidase found in the mushroom Grifola frondosa that cleaves proteins on the amino side of lysine residues.[1]

Crystal structure of Lys-N with co-ordinated zinc atom.[2]

Mass spectrometry

Lys-N is becoming a popular protease used for protein digestion in proteomics experiments. The combination Lys-N proteolytic peptides and mass spectrometry sequencing with ETD creates tandem mass spectra composed mostly of amino terminal peptide fragment ions.[3] This fragmentation pattern facilitates the applicability of these spectra for de novo peptide sequencing.[3]

See also

References

  1. ↑ Nonaka T, Hashimoto Y, Takio K (July 1998). "Kinetic characterization of lysine-specific metalloendopeptidases from Grifola frondosa and Pleurotus ostreatus fruiting bodies". Journal of Biochemistry 124 (1): 157–62. doi:10.1093/oxfordjournals.jbchem.a022074. PMID 9644258.
  2. ↑ RCSB Protein Data Bank - RCSB PDB - 1G12 Structure Summary
  3. ↑ 3.0 3.1 Taouatas N, Drugan MM, Heck AJ, Mohammed S (May 2008). "Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase". Nature Methods 5 (5): 405–7. doi:10.1038/nmeth.1204. PMID 18425140.

External links