Lupeol synthase

Lupeol synthase
Identifiers
EC number 5.4.99.41
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Lupeol synthase (EC 5.4.99.41, LUPI, BPW, RcLUS) is an enzyme with system name (3S)-2,3-epoxy-2,3-dihydrosqualene mutase (cyclizing, lupeol-forming).[1][2][3][4][5][6][7] This enzyme catalyses the following chemical reaction

(3S)-2,3-epoxy-2,3-dihydrosqualene \rightleftharpoons lupeol

Also forms some beta-amyrin.

References

  1. ↑ Herrera, J.B., Bartel, B., Wilson, W.K. and Matsuda, S.P. (1998). "Cloning and characterization of the Arabidopsis thaliana lupeol synthase gene". Phytochemistry 49: 1905–1911. doi:10.1016/s0031-9422(98)00366-5. PMID 9883589.
  2. ↑ Shibuya, M., Zhang, H., Endo, A., Shishikura, K., Kushiro, T. and Ebizuka, Y. (1999). "Two branches of the lupeol synthase gene in the molecular evolution of plant oxidosqualene cyclases". Eur. J. Biochem. 266: 302–307. doi:10.1046/j.1432-1327.1999.00875.x. PMID 10542078.
  3. ↑ Segura, M.J., Meyer, M.M. and Matsuda, S.P. (2000). "Arabidopsis thaliana LUP1 converts oxidosqualene to multiple triterpene alcohols and a triterpene diol". Org. Lett. 2: 2257–2259. doi:10.1021/ol006016b. PMID 10930257.
  4. ↑ Zhang, H., Shibuya, M., Yokota, S. and Ebizuka, Y. (2003). "Oxidosqualene cyclases from cell suspension cultures of Betula platyphylla var. japonica: molecular evolution of oxidosqualene cyclases in higher plants". Biol. Pharm. Bull. 26: 642–650. doi:10.1248/bpb.26.642. PMID 12736505.
  5. ↑ Hayashi, H., Huang, P., Takada, S., Obinata, M., Inoue, K., Shibuya, M. and Ebizuka, Y. (2004). "Differential expression of three oxidosqualene cyclase mRNAs in Glycyrrhiza glabra". Biol. Pharm. Bull. 27: 1086–1092. doi:10.1248/bpb.27.1086. PMID 15256745.
  6. ↑ Guhling, O., Hobl, B., Yeats, T. and Jetter, R. (2006). "Cloning and characterization of a lupeol synthase involved in the synthesis of epicuticular wax crystals on stem and hypocotyl surfaces of Ricinus communis". Arch. Biochem. Biophys. 448: 60–72. doi:10.1016/j.abb.2005.12.013. PMID 16445885.
  7. ↑ Basyuni, M., Oku, H., Tsujimoto, E., Kinjo, K., Baba, S. and Takara, K. (2007). "Triterpene synthases from the Okinawan mangrove tribe, Rhizophoraceae". FEBS J. 274: 5028–5042. doi:10.1111/j.1742-4658.2007.06025.x. PMID 17803686.

External links