Lecithin retinol acyltransferase
Lecithin retinol acyltransferase is an enzyme that in humans is encoded by the LRAT gene.[1][2]
Function
Lecithin retinol acyltransferase is a microsomal enzyme that catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester during phototransduction, an essential reaction for the retinoid cycle in visual system and vitamin A status in liver.
Clinical significance
Mutations in this gene have been associated with early-onset severe retinal dystrophy.[2]
LRAT was overexpressed in colorectal cancer cells compared to normal colonic epithelium. Strong LRAT expression was associated with a poor prognosis in patients with colorectal cancer.[3]
See also
- The Visual Cycle
References
- ↑ Ruiz A, Winston A, Lim YH, Gilbert BA, Rando RR, Bok D (Feb 1999). "Molecular and biochemical characterization of lecithin retinol acyltransferase". J Biol Chem 274 (6): 3834–41. doi:10.1074/jbc.274.6.3834. PMID 9920938.
- ↑ 2.0 2.1 "Entrez Gene: LRAT lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)".
- ↑ Brown, Gordon; Beatriz Cash; Daniela Blihoghe; Petronella Johansson; Ayham Alnabulsi; Graeme Murray (2014-03-07). "The Expression and Prognostic Significance of Retinoic Acid Metabolising Enzymes in Colorectal Cancer". PLOS ONE. doi:10.1371/journal.pone.0090776.
Further reading
- Ross AC, Zolfaghari R (2004). "Regulation of hepatic retinol metabolism: perspectives from studies on vitamin A status.". J. Nutr. 134 (1): 269S–275S. PMID 14704332.
- Herr FM, Ong DE (1992). "Differential interaction of lecithin-retinol acyltransferase with cellular retinol binding proteins.". Biochemistry 31 (29): 6748–55. doi:10.1021/bi00144a014. PMID 1322170.
- Mata NL, Tsin AT (1998). "Distribution of 11-cis LRAT, 11-cis RD and 11-cis REH in bovine retinal pigment epithelium membranes.". Biochim. Biophys. Acta 1394 (1): 16–22. doi:10.1016/s0005-2760(98)00078-2. PMID 9767084.
- Mondal MS, Ruiz A, Bok D, Rando RR (2000). "Lecithin retinol acyltransferase contains cysteine residues essential for catalysis.". Biochemistry 39 (17): 5215–20. doi:10.1021/bi9929554. PMID 10819989.
- Ruiz A, Kuehn MH, Andorf JL, et al. (2001). "Genomic organization and mutation analysis of the gene encoding lecithin retinol acyltransferase in human retinal pigment epithelium.". Invest. Ophthalmol. Vis. Sci. 42 (1): 31–7. PMID 11133845.
- Andreola F, Giandomenico V, Spero R, De Luca LM (2001). "Expression of a smaller lecithin:retinol acyl transferase transcript and reduced retinol esterification in MCF-7 cells.". Biochem. Biophys. Res. Commun. 279 (3): 920–4. doi:10.1006/bbrc.2000.3995. PMID 11162450.
- Thompson DA, Li Y, McHenry CL, et al. (2001). "Mutations in the gene encoding lecithin retinol acyltransferase are associated with early-onset severe retinal dystrophy.". Nat. Genet. 28 (2): 123–4. doi:10.1038/88828. PMID 11381255.
- Jahng WJ, Cheung E, Rando RR (2002). "Lecithin retinol acyltransferase forms functional homodimers.". Biochemistry 41 (20): 6311–9. doi:10.1021/bi015710b. PMID 12009892.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Zhan HC, Gudas LJ, Bok D, et al. (2004). "Differential expression of the enzyme that esterifies retinol, lecithin:retinol acyltransferase, in subtypes of human renal cancer and normal kidney.". Clin. Cancer Res. 9 (13): 4897–905. PMID 14581364.
- Jahng WJ, Xue L, Rando RR (2004). "Lecithin retinol acyltransferase is a founder member of a novel family of enzymes.". Biochemistry 42 (44): 12805–12. doi:10.1021/bi035370p. PMID 14596594.
- Boorjian S, Tickoo SK, Mongan NP, et al. (2004). "Reduced lecithin: retinol acyltransferase expression correlates with increased pathologic tumor stage in bladder cancer.". Clin. Cancer Res. 10 (10): 3429–37. doi:10.1158/1078-0432.CCR-03-0756. PMID 15161698.
- Zolfaghari R, Ross AC (2005). "Cloning, gene organization and identification of an alternative splicing process in lecithin:retinol acyltransferase cDNA from human liver.". Gene 341: 181–8. doi:10.1016/j.gene.2004.06.043. PMID 15474300.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- O'Byrne SM, Wongsiriroj N, Libien J, et al. (2005). "Retinoid absorption and storage is impaired in mice lacking lecithin:retinol acyltransferase (LRAT).". J. Biol. Chem. 280 (42): 35647–57. doi:10.1074/jbc.M507924200. PMC 1352312. PMID 16115871.
- Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
- Xue L, Jahng WJ, Gollapalli D, Rando RR (2006). "Palmitoyl transferase activity of lecithin retinol acyl transferase.". Biochemistry 45 (35): 10710–8. doi:10.1021/bi060897y. PMC 2529161. PMID 16939223.
- Sénéchal A, Humbert G, Surget MO, et al. (2006). "Screening genes of the retinoid metabolism: novel LRAT mutation in leber congenital amaurosis.". Am. J. Ophthalmol. 142 (4): 702–4. doi:10.1016/j.ajo.2006.04.057. PMID 17011878.