James Knowles

James Knowles may refer to:

• James Sheridan Knowles (1784–1862), Irish dramatist and actor
• James Thomas Knowles (1806–1884), English architect
• James Knowles (architect) (1831–1908), English architect
• James Knowles (aviator) (1896–1971), World War I ace
• Jim Knowles (American football), head football coach at Cornell University
• Jim Knowles (football manager), manager of Tranmere Rovers, 1936–1939
• Jimmy Knowles (1856–1912), baseball player
• James Knowles, Ku Klux Klan member responsible for the lynching of Michael Donald
• James Knowles III, Mayor of Ferguson, Missouri