Funnel plot

An example funnel plot showing no publication bias. Each dot represents a study (e.g. measuring the effect of a certain drug); the y-axis represents the size of the study (e.g. number of experimental subjects) and the x-axis shows the study's result (e.g. the drug's measured average effect).

A funnel plot is a graph designed to check for the existence of publication bias; funnel plots are commonly used in systematic reviews and meta-analyses. In the absence of publication bias, it assumes that the largest studies will be plotted near the average, and smaller studies will be spread evenly on both sides of the average, creating a roughly funnel-shaped distribution. Deviation from this shape can indicate publication bias.

Quotation

Funnel plots, introduced by Light and Pillemer in 1984[1] and discussed in detail by Egger and colleagues,[2][3] are useful adjuncts to meta-analyses. A funnel plot is a scatterplot of treatment effect against a measure of study size. It is used primarily as a visual aid for detecting bias or systematic heterogeneity. A symmetric inverted funnel shape arises from a ‘well-behaved’ data set, in which publication bias is unlikely. An asymmetric funnel indicates a relationship between treatment effect and study size. This suggests the possibility of either publication bias or a systematic difference between smaller and larger studies (‘small study effects’). Asymmetry can also arise from use of an inappropriate effect measure. Whatever the cause, an asymmetric funnel plot leads to doubts over the appropriateness of a simple meta-analysis and suggests that there needs to be investigation of possible causes.

A variety of choices of measures of ‘study size’ is available, including total sample size, standard error of the treatment effect, and inverse variance of the treatment effect (weight). Sterne and Egger have compared these with others, and conclude that the standard error is to be recommended.[3] When the standard error is used, straight lines may be drawn to define a region within which 95% of points might lie in the absence of both heterogeneity and publication bias.[3]

In common with confidence interval plots, funnel plots are conventionally drawn with the treatment effect measure on the horizontal axis, so that study size appears on the vertical axis, breaking with the general rule. Since funnel plots are principally visual aids for detecting asymmetry along the treatment effect axis, this makes them considerably easier to interpret.

Criticism

The funnel plot is not without problems. If high precision studies really are different from low precision studies with respect to effect size (e.g., due to different populations examined) a funnel plot may give a wrong impression of publication bias.[4] The appearance of the funnel plot can change quite dramatically depending on the scale on the y-axis — whether it is the inverse square error or the trial size.[5]

See also

References

  1. ↑ R. J. Light, D. B. Pillemer (1984). Summing up: The Science of Reviewing Research. Cambridge, Massachusetts.: Harvard University Press. ISBN 0-674-85431-4.
  2. ↑ Matthias Egger, G. Davey Smith, M. Schneider & C. Minder (September 1997). "Bias in meta-analysis detected by a simple, graphical test". BMJ 315 (7109): 629–624. doi:10.1136/bmj.315.7109.629. PMC 2127453. PMID 9310563.
  3. ↑ 3.0 3.1 3.2 Jonathan A. C. Sterne & Matthias Egger (October 2001). "Funnel plots for detecting bias in meta-analysis: guidelines on choice of axis". Journal of Clinical Epidemiology 54 (10): 1046–1045. doi:10.1016/S0895-4356(01)00377-8. PMID 11576817.
  4. ↑ Joseph Lau, John P. A. Ioannidis, Norma Terrin, Christopher H. Schmid & Ingram Olkin (September 2006). "The case of the misleading funnel plot". BMJ 333 (7568): 597–590. doi:10.1136/bmj.333.7568.597. PMC 1570006. PMID 16974018.
  5. ↑ Jin-Ling Tang, Joseph LY Liu (May 2000). "Misleading funnel plot for detection of bias in meta-analysis". Journal of Clinical Epidemiology 53 (5): 477–484. doi:10.1016/S0895-4356(99)00204-8. PMID 10812319.

Further reading