Fluo-4

Fluo-4

Names
IUPAC name Fluo-4
Systematic IUPAC name 2-{[2-(2-{5-[bis(carboxymethyl)amino]-2-methylphenoxy}ethoxy)-4-(2,7-difluoro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenyl](carboxymethyl)amino}acetic acid
Identifiers
ChEBI	CHEBI:51103
InChI InChI=1S/C36H30F2N2O13/c1-18-2-4-20(39(14-32(43)44)15-33(45)46)9-28(18)51-6-7-52-31-8-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-21-10-23(37)26(41)12-29(21)53-30-13-27(42)24(38)11-22(30)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50) Y Key: WZZRKOQVHRRNDH-UHFFFAOYSA-N Y
Jmol-3D images	Image Image
MeSH	Fluo-4
SMILES CC1=C(OCCOC2=C(C=CC(=C2)C2=C3C=C(F)C(=O)C=C3OC3=CC(O)=C(F)C=C23)N(CC(O)=O)CC(O)=O)C=C(C=C1)N(CC(O)=O)CC(O)=O CC1=CC=C(C=C1OCCOC1=C(C=CC(=C1)C1=C2C=C(F)C(=O)C=C2OC2=C1C=C(F)C(O)=C2)N(CC(O)=O)CC(O)=O)N(CC(O)=O)CC(O)=O
Properties
Molecular formula	C36H30F2N2O13
Molar mass	736.63 g·mol−1
Except where noted otherwise, data is given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
Infobox references

Fluo-4 is used to measure calcium (Ca²⁺) concentrations inside living cells, and is often used for high-throughput screening of receptor ligands and calcium permeable ion channels.

The green-fluorescent calcium indicator, Fluo-4, is an improved version of the calcium indicator, Fluo-3. It is commonly used as the non-fluorescent acetoxymethyl ester (Fluo-4 AM) which is cleaved inside the cell to give the free, fluorescent Fluo-4. It loads faster, is brighter at equivalent concentrations and is well-excited by the 488 nm line of the argon-ion laser which is often used in biological research laboratories.^[1] The sole distributor and owner of the patents is Invitrogen which acquired the original inventor Molecular Probes.

References