Felisa Wolfe-Simon

Felisa Lauren Wolfe-Simon
Wolfe-Simon at the 2011 Time 100 gala
Born	Felisa Lauren Wolfe
Residence	United States
Fields	Biochemistry Microbiology Astrobiology Geochemistry Geomicrobiology Oceanography
Institutions	Lawrence Berkeley National Laboratory NASA Astrobiology Institute US Geological Survey Rutgers University
Alma mater	Rutgers University Institute of Marine and Coastal Sciences (Ph.D.) Oberlin College (B.A.) Oberlin Conservatory of Music (B.M.)
Known for	GFAJ-1 bacterium

Felisa Wolfe-Simon is an American microbial geobiologist and biogeochemist. In 2010, Wolfe-Simon led a team that discovered GFAJ-1, an extremophile bacterium that they claimed was capable of substituting arsenic for a small percentage of its phosphorus to sustain its growth, thus advancing the remarkable possibility of non-RNA/DNA-based genetics.^[1] However, these conclusions were immediately debated and critiqued in correspondence to the original journal of publication,^[2] and have since come to be widely disbelieved.^[3] In 2012, two reports refuting the most significant aspects of the original results were published in the same journal in which the original findings had been previously published.^[4][5]

Education and career

Wolfe-Simon did her undergraduate studies at Oberlin College and completed a Bachelor of Arts in Biology and Chemistry and a Bachelor of Music in Oboe Performance and Ethnomusicology at the Oberlin Conservatory of Music.^[6] She received her Doctor of Philosophy in oceanography from the Institute of Marine and Coastal Sciences at Rutgers University in 2006 with a dissertation titled The Role and Evolution of Superoxide Dismutases in Algae.^[7] Later Wolfe-Simon was a NASA research fellow in residence at the US Geological Survey and a member of the NASA Astrobiology Institute. She is currently at Lawrence Berkeley National Laboratory.^[8]

Controversy

Wolfe-Simon's research focuses on evolutionary microbiology and exotic metabolic pathways. At a conference in 2008 and subsequent 2009 paper, Wolfe-Simon, Paul Davies and Ariel Anbar proposed that arsenate (AsO3−
4) could serve as a substitute for phosphate (PO3−
4) in various forms of biochemistry.^[9][10] According to Paul Davies, Wolfe-Simon was the one who had the "critical insight" that arsenic might be able to substitute for phosphorus.^[11] As late as March 2010, she had been hinting of some shadow biosphere results to the press.^[12][13]

Wolfe-Simon processing mud at Mono Lake, 2010

Wolfe-Simon then led a search for such an organism by targeting the naturally occurring arsenic-rich Mono Lake, California. This search led to the discovery of the bacterium GFAJ-1, which her team claimed in a Science on-line article in December 2010 was able to incorporate arsenate as a substitute for a small percentage of the typical phosphate in its DNA and other essential biomolecules.^[1] If correct, this would be the only known organism to be capable of replacing phosphorus in its DNA and other vital biochemical functions.^[14][15][16] The Science publication and an hour-long December 2, 2010 NASA news conference were publicized and led to "wild speculations on the Web about extraterrestrial life".^[17] Wolfe-Simon was the only one of the paper's authors at that news conference.^[18] The news conference was promptly met with criticism by scientists and journalists.^[19] In the following month, Wolfe-Simon (and her co-authors and NASA) responded to criticisms through an online FAQ and an exclusive interview with a Science reporter, but also announced they would not respond further outside scientific peer-review.^{[20][21][22][23][24][25]} Wolfe-Simon left USGS in May 2011 to pursue her research elsewhere.^[26] Wolfe-Simon maintains she did not leave voluntarily, but was "effectively evicted" from the USGS group.^[27]

The Science article "A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus" appeared in the June 3, 2011 print version of Science;^[1] it had remained on the "Publication ahead of print" ScienceXpress page for six months after acceptance for publication.^[28]

However, researchers from the University of British Columbia and Princeton University performed studies in which they used a variety of different techniques to investigate the presence of arsenic in the DNA of GFAJ-1 and published their results in early 2012. The group found no detectable arsenic in the DNA of the bacterium. In addition they found that the strain did not grow in the presence of arsenate, further supporting the absence of the element and its lack of participation in essential biological processes.^[29][30]

Following the publication of the articles challenging the conclusions of the original Science article first describing GFAJ-1 the website Retraction Watch argued that the original article should be retracted because of misrepresentation of critical data.^[31][32] As of April 2013, no retraction had been announced.

Recognition

In 2006 Wolfe-Simon was awarded a National Science Foundation Minority Postdoctoral Research Fellowship ^[33] to support work done at Harvard University and Arizona State University. In 2010, she received a Kavli Fellowship from the United States National Academy of Sciences.

Publications

• F. Wolfe, K. Kroeger and I. Valiela (1999). Increased lability of estuarine dissolved organic nitrogen from urbanized watersheds. Biological Bulletin. 197:290-292.
• Wolfe-Simon F., Grzebyk D., Schofield O., Falkowski P. G. (2005). "The role and evolution of superoxide dismutase in algae". Journal of Phycology 41 (3): 453–465. doi:10.1111/j.1529-8817.2005.00086.x. 
• F. Wolfe-Simon (2006). The Role and Evolution of Superoxide Dismutases in Algae. Ph.D. Thesis. Rutgers Graduate Program in Oceanography.
• Wolfe-Simon F., Starovoytov V., Reinfelder J.R., Schofield O., Falkowski P. G. (2006). "Localization and role of manganese superoxide dismutase in a marine diatom". Plant Physiology 142 (4): 1701–1709. doi:10.1104/pp.106.088963. PMC 1676035. PMID 17056755. 
• Davies P.C.W., Benner S.A., Cleland C.E., Lineweaver C.H., McKay C.P., Wolfe-Simon F. (2009). "Signatures of a Shadow Biosphere". Astrobiology 9 (2): 241–249. Bibcode:2009AsBio...9..241D. doi:10.1089/ast.2008.0251. PMID 19292603. 
• J.B. Glass, F. Wolfe-Simon, and A.D. Anbar (2009). Coevolution of marine metal availability and nitrogen assimilation in cyanobacteria and algae. Geobiology. 7: 100-123.
• F. Wolfe-Simon, P.C.W. Davies and A.D. Anbar (2009). Did nature also choose Arsenic? International Journal of Astrobiology. 8: 69-74.
• R.S. Oremland, C.W. Saltikov, F. Wolfe-Simon, and J.F. Stolz (2009). Arsenic in the evolution of Earth and extraterrestrial ecosystems. Geomicrobiology Journal. 26: 522 - 536.
• Johnston D.T., Wolfe-Simon F., Pearson A., Knoll A.H. (2009). "Anoxygenic photosynthesis modulated Proterozoic oxygen and sustained Earth's middle age". Proceedings of the National Academy of Sciences 106 (40): 16925–16929. Bibcode:2009PNAS..10616925J. doi:10.1073/pnas.0909248106. PMC 2753640. PMID 19805080. 
• J.B. Glass, F. Wolfe-Simon, J.J. Elser and A.D. Anbar (2010). Molybdenum-nitrogen colimitation in heterocystous cyanobacteria. Limnology and Oceanography. 55: 667-676.
• Chauhan D, Folea IM, Jolley CC et al. (February 2011). "A Novel Photosynthetic Strategy for Adaptation to Low-Iron Aquatic Environments". Biochemistry 50 (5): 686–692. doi:10.1021/bi1009425. PMID 20942381.  CS1 maint: Explicit use of et al. (link)
• Felisa Wolfe-Simon, Jodi Switzer Blum, Thomas R. Kulp, Gwyneth W. Gordon, Shelley E. Hoeft, Jennifer Pett-Ridge, John F. Stolz, Samuel M. Webb, Peter K. Weber, Paul C. W. Davies, Ariel D. Anbar and Ronald S. Oremland (2010). "A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus". Science 332 (6034): 1163–6. Bibcode:2011Sci...332.1163W. doi:10.1126/science.1197258. PMID 21127214. 
• Wolfe-Simon, Felisa; Blum; Kulp, Thomas R.; Gordon, Gwyneth W.; Hoeft, Shelley E.; Pett-Ridge, Jennifer; Stolz, John F.; Webb, Samuel M.; Weber, Peter K.; Davies, Paul C. W.; Anbar, Ariel D. (27 May 2011). "Response to Comments on "A Bacterium That Can Grow Using Arsenic Instead of Phosphorus"" (PDF). Science. Bibcode:2011Sci...332.1149W. doi:10.1126/science.1202098. Retrieved 30 May 2011. 

See also

• Hypothetical types of biochemistry
• Prebiotic arsenic

References

External links

Wikimedia Commons has media related to Felisa Wolfe-Simon.

• Official website
• Labyrint on Astrobiology. Dutch science documentary featuring Wolfe-Simon. six-minute segment with Wolfe-Simon.
• NASA-funded research discovers life built with toxic chemical one-hour press conference on NASA TV 2010-12-02, with Wolfe-Simon, Mary Voytek, Steven A. Benner, Pamela Conrad and James Elser