Eukaryotic translation initiation factor 4 gamma

Eukaryotic translation initiation factor 4 gamma, 1

PDB rendering based on 1ug3.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
1LJ2, 1UG3, 2W97, 4F02

Identifiers

Symbols

EIF4G1 ; EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18

External IDs

OMIM: 600495 MGI: 2384784 HomoloGene: 110725 ChEMBL: 1741197 GeneCards: EIF4G1 Gene

Gene ontology
Molecular function	• translation initiation factor activity • protein binding • translation factor activity, nucleic acid binding • poly(A) RNA binding
Cellular component	• cytosol • membrane • eukaryotic translation initiation factor 4F complex
Biological process	• nuclear-transcribed mRNA catabolic process, nonsense-mediated decay • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay • nuclear-transcribed mRNA poly(A) tail shortening • translation • translational initiation • regulation of translational initiation • cell death • insulin receptor signaling pathway • gene expression • viral process • RNA metabolic process • mRNA metabolic process • cytokine-mediated signaling pathway • cellular protein metabolic process
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 3:
184.03 – 184.05 Mb

Chr 16:
20.67 – 20.69 Mb

PubMed search

Eukaryotic translation initiation factor 4 gamma 1 is a protein that in humans is encoded by the EIF4G1 gene.^[1]^[2]

The protein encoded by this gene is a component of the protein complex EIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure, and recruitment of mRNA to the ribosome. Alternative splicing results in five transcript variants encoding four distinct isoforms.^[3]

Interactions

Eukaryotic translation initiation factor 4 gamma has been shown to interact with MKNK1,^[4] EIF4A1,^[5]^[6]^[7] EIF4E,^[6]^[7]^[8]^[9]^[10] MKNK2^[11] and PABPC1.^[12]

References

↑ Yan R, Rychlik W, Etchison D, Rhoads RE (December 1992). "Amino acid sequence of the human protein synthesis initiation factor eIF-4 gamma". J Biol Chem 267 (32): 23226–31. PMID 1429670.
↑ Imataka H, Sonenberg N (December 1997). "Human eukaryotic translation initiation factor 4G (eIF4G) possesses two separate and independent binding sites for eIF4A". Mol Cell Biol 17 (12): 6940–7. PMC 232551. PMID 9372926.
↑ "Entrez Gene: EIF4G1 eukaryotic translation initiation factor 4 gamma, 1".
↑ Pyronnet, S; Imataka H; Gingras A C; Fukunaga R; Hunter T; Sonenberg N (January 1999). "Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E". EMBO J. (ENGLAND) 18 (1): 270–9. doi:10.1093/emboj/18.1.270. ISSN 0261-4189. PMC 1171121. PMID 9878069.
↑ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
↑ 6.0 6.1 Connolly, Eileen; Braunstein Steve; Formenti Silvia; Schneider Robert J (May 2006). "Hypoxia inhibits protein synthesis through a 4E-BP1 and elongation factor 2 kinase pathway controlled by mTOR and uncoupled in breast cancer cells". Mol. Cell. Biol. (United States) 26 (10): 3955–65. doi:10.1128/MCB.26.10.3955-3965.2006. ISSN 0270-7306. PMC 1489005. PMID 16648488.
↑ 7.0 7.1 Harris, Thurl E; Chi An; Shabanowitz Jeffrey; Hunt Donald F; Rhoads Robert E; Lawrence John C (April 2006). "mTOR-dependent stimulation of the association of eIF4G and eIF3 by insulin". EMBO J. (England) 25 (8): 1659–68. doi:10.1038/sj.emboj.7601047. ISSN 0261-4189. PMC 1440840. PMID 16541103.
↑ Vary, T C; Jefferson L S; Kimball S R (December 1999). "Amino acid-induced stimulation of translation initiation in rat skeletal muscle". Am. J. Physiol. (UNITED STATES) 277 (6 Pt 1): E1077–86. ISSN 0002-9513. PMID 10600798.
↑ Mader, S; Lee H; Pause A; Sonenberg N (September 1995). "The translation initiation factor eIF-4E binds to a common motif shared by the translation factor eIF-4 gamma and the translational repressors 4E-binding proteins". Mol. Cell. Biol. (UNITED STATES) 15 (9): 4990–7. ISSN 0270-7306. PMC 230746. PMID 7651417.
↑ Kumar, V; Sabatini D, Pandey P, Gingras A C, Majumder P K, Kumar M, Yuan Z M, Carmichael G, Weichselbaum R, Sonenberg N, Kufe D, Kharbanda S (April 2000). "Regulation of the rapamycin and FKBP-target 1/mammalian target of rapamycin and cap-dependent initiation of translation by the c-Abl protein-tyrosine kinase". J. Biol. Chem. (UNITED STATES) 275 (15): 10779–87. doi:10.1074/jbc.275.15.10779. ISSN 0021-9258. PMID 10753870.
↑ Scheper, Gert C; Parra Josep L; Wilson Mary; Van Kollenburg Barbara; Vertegaal Alfred C O; Han Ze-Guang; Proud Christopher G (August 2003). "The N and C termini of the splice variants of the human mitogen-activated protein kinase-interacting kinase Mnk2 determine activity and localization". Mol. Cell. Biol. (United States) 23 (16): 5692–705. doi:10.1128/MCB.23.16.5692-5705.2003. ISSN 0270-7306. PMC 166352. PMID 12897141.
↑ Imataka, H; Gradi A; Sonenberg N (December 1998). "A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation". EMBO J. (ENGLAND) 17 (24): 7480–9. doi:10.1093/emboj/17.24.7480. ISSN 0261-4189. PMC 1171091. PMID 9857202.

Gingras AC, Raught B, Sonenberg N (2000). "eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation.". Annu. Rev. Biochem. 68: 913–63. doi:10.1146/annurev.biochem.68.1.913. PMID 10872469.
Yan R, Rhoads RE (1995). "Human protein synthesis initiation factor eIF-4 gamma is encoded by a single gene (EIF4G) that maps to chromosome 3q27-qter.". Genomics 26 (2): 394–8. doi:10.1016/0888-7543(95)80227-D. PMID 7601469.
Mader S, Lee H, Pause A, Sonenberg N (1995). "The translation initiation factor eIF-4E binds to a common motif shared by the translation factor eIF-4 gamma and the translational repressors 4E-binding proteins.". Mol. Cell. Biol. 15 (9): 4990–7. PMC 230746. PMID 7651417.
Pause A, Belsham GJ, Gingras AC et al. (1994). "Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function.". Nature 371 (6500): 762–7. doi:10.1038/371762a0. PMID 7935836.
Lamphear BJ, Yan R, Yang F et al. (1993). "Mapping the cleavage site in protein synthesis initiation factor eIF-4 gamma of the 2A proteases from human Coxsackievirus and rhinovirus.". J. Biol. Chem. 268 (26): 19200–3. PMID 8396129.
Haghighat A, Mader S, Pause A, Sonenberg N (1996). "Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E.". EMBO J. 14 (22): 5701–9. PMC 394685. PMID 8521827.
Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery.". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
Lamphear BJ, Rhoads RE (1997). "A single amino acid change in protein synthesis initiation factor 4G renders cap-dependent translation resistant to picornaviral 2A proteases.". Biochemistry 35 (49): 15726–33. doi:10.1021/bi961864t. PMID 8961935.
Brass N, Heckel D, Sahin U et al. (1997). "Translation initiation factor eIF-4gamma is encoded by an amplified gene and induces an immune response in squamous cell lung carcinoma.". Hum. Mol. Genet. 6 (1): 33–9. doi:10.1093/hmg/6.1.33. PMID 9002667.
Eberle J, Krasagakis K, Orfanos CE (1997). "Translation initiation factor eIF-4A1 mRNA is consistently overexpressed in human melanoma cells in vitro.". Int. J. Cancer 71 (3): 396–401. doi:10.1002/(SICI)1097-0215(19970502)71:3<396::AID-IJC16>3.0.CO;2-E. PMID 9139875.
Gradi A, Imataka H, Svitkin YV et al. (1998). "A novel functional human eukaryotic translation initiation factor 4G.". Mol. Cell. Biol. 18 (1): 334–42. PMC 121501. PMID 9418880.
Piron M, Vende P, Cohen J, Poncet D (1998). "Rotavirus RNA-binding protein NSP3 interacts with eIF4GI and evicts the poly(A) binding protein from eIF4F.". EMBO J. 17 (19): 5811–21. doi:10.1093/emboj/17.19.5811. PMC 1170909. PMID 9755181.
Imataka H, Gradi A, Sonenberg N (1999). "A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation.". EMBO J. 17 (24): 7480–9. doi:10.1093/emboj/17.24.7480. PMC 1171091. PMID 9857202.
Pyronnet S, Imataka H, Gingras AC et al. (1999). "Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E.". EMBO J. 18 (1): 270–9. doi:10.1093/emboj/18.1.270. PMC 1171121. PMID 9878069.
Laroia G, Cuesta R, Brewer G, Schneider RJ (1999). "Control of mRNA decay by heat shock-ubiquitin-proteasome pathway.". Science 284 (5413): 499–502. doi:10.1126/science.284.5413.499. PMID 10205060.
Vary TC, Jefferson LS, Kimball SR (2000). "Amino acid-induced stimulation of translation initiation in rat skeletal muscle.". Am. J. Physiol. 277 (6 Pt 1): E1077–86. PMID 10600798.
Cuesta R, Laroia G, Schneider RJ (2000). "Chaperone hsp27 inhibits translation during heat shock by binding eIF4G and facilitating dissociation of cap-initiation complexes.". Genes Dev. 14 (12): 1460–70. PMC 316692. PMID 10859165.
Chartier-Harlin M, Dachsel JC, Vilariño-Güell C et al. (2011). "Translation Initiator EIF4G1 Mutations in Familial Parkinson Disease.". American Journal of Human Genetics 89 (3): 398–406. doi:10.1016/j.ajhg.2011.08.009.

PDB gallery

1ug3: C-terminal portion of human eIF4GI

Protein biosynthesis: translation (prokaryotic, eukaryotic)

Ribosomal proteins

Initiation factor

Prokaryotic

Archaeal

aIF1
aIF2
aIF5
aIF6

Eukaryotic

eIF1	EIF1AX AY 1B

eIF2	EIF2S1 EIF2S2 EIF2S3 EIF2B1 EIF2B2 EIF2B3 EIF2B4 EIF2B5 EIF-2 kinase

eIF3	EIF3A B C D F G H I J K M S6

eIF4	EIF4A2 A3 B E1 E2 G1 G2 G3 H

eIF5	EIF5A A2 B

eIF6	EIF6

Elongation factor

Prokaryotic	EF-Tu EF-Ts EF-G

Archaeal	aEF-1, aEF-2

Eukaryotic	EEF-1 EEF1A1 EEF1A2 EEF1A3 EEF1B1 EEF1B2 EEF1B3 EEF1B4 EEF1D EEF1E1 EEF1G EEF2

Release factor

Other

Other concepts

Index of genetics

Description	Gene expression DNA replication cycle recombination repair binding proteins Transcription factors regulators nucleic acids RNA RNA binding proteins ribonucleoproteins repeated sequence modification Translation ribosome modification nexins Proteins domains Structure primary secondary tertiary quaternary

Disease	Replication and repair Transcription factor Transcription Translation

Eukaryotic translation initiation factor 4 gamma

Interactions

See also

References

Further reading