Ent-kaurenoic acid oxidase

Ent-kaurenoic acid oxidase
Identifiers
EC number 1.14.13.79
CAS number 337507-95-6
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Ent-kaurenoic acid oxidase (EC 1.14.13.79) is an enzyme with system name ent-kaur-16-en-19-oate,NADPH:oxygen oxidoreductase (hydroxylating).[1] This enzyme catalyses the following chemical reaction

ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2 \rightleftharpoons gibberellin A12 + 3 NADP+ + 4 H2O (overall reaction)
(1a) ent-kaur-16-en-19-oate + NADPH + H+ + O2 \rightleftharpoons ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
(1b) ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + H+ + O2 \rightleftharpoons gibberellin A12 aldehyde + NADP+ + 2 H2O
(1c) gibberellin A12 aldehyde + NADPH + H+ + O2 \rightleftharpoons gibberellin A12 + NADP+ + H2O

Ent-kaurenoic acid oxidase requires cytochrome P450.

References

  1. ↑ Helliwell, C.A., Chandler, P.M., Poole, A., Dennis, E.S. and Peacock, W.J. (2001). "The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway". Proc. Natl. Acad. Sci. USA 98 (4): 2065–2070. doi:10.1073/pnas.041588998. PMC 29382. PMID 11172076.

External links