EIF4A1

Eukaryotic translation initiation factor 4A1

PDB rendering based on 2g9n.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
2G9N, 2ZU6, 3EIQ

Identifiers

EIF4A1 ; DDX2A; EIF-4A; EIF4A; eIF-4A-I; eIF4A-I

External IDs

OMIM: 602641 MGI: 95303 HomoloGene: 103998 GeneCards: EIF4A1 Gene

Gene ontology
Molecular function	• RNA cap binding • double-stranded RNA binding • mRNA binding • translation initiation factor activity • helicase activity • protein binding • ATP binding • ATP-dependent helicase activity • translation factor activity, nucleic acid binding • poly(A) RNA binding
Cellular component	• cytosol • membrane • eukaryotic translation initiation factor 4F complex • extracellular vesicular exosome
Biological process	• nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay • nuclear-transcribed mRNA poly(A) tail shortening • ATP catabolic process • translation • translational initiation • gene expression • viral process • RNA metabolic process • mRNA metabolic process • cytokine-mediated signaling pathway • organ regeneration • cellular protein metabolic process
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

ENSG00000161960

ENSMUSG00000059796

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 17:
7.48 – 7.48 Mb

Chr 11:
69.67 – 69.67 Mb

PubMed search

Eukaryotic initiation factor 4A-I is a protein that in humans is encoded by the EIF4A1 gene.^[1]^[2]^[3]

Interactions

EIF4A1 has been shown to interact with EIF4E^[4]^[5] and Eukaryotic translation initiation factor 4 gamma.^[4]^[5]^[6]

References

↑ Kim NS, Kato T, Abe N, Kato S (Jun 1993). "Nucleotide sequence of human cDNA encoding eukaryotic initiation factor 4AI". Nucleic Acids Res. 21 (8): 2012. doi:10.1093/nar/21.8.2012. PMC 309447. PMID 8493113. CS1 maint: Date and year (link)
↑ Jones E, Quinn CM, See CG, Montgomery DS, Ford MJ, Kölble K et al. (Dec 1998). "The linked human elongation initiation factor 4A1 (EIF4A1) and CD68 genes map to chromosome 17p13". Genomics 53 (2): 248–50. doi:10.1006/geno.1998.5515. PMID 9790779. Vancouver style error (help) CS1 maint: Date and year (link)
↑ "Entrez Gene: EIF4A1 eukaryotic translation initiation factor 4A, isoform 1".
↑ 4.0 4.1 Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
↑ 5.0 5.1 Connolly E, Braunstein S, Formenti S, Schneider RJ (May 2006). "Hypoxia inhibits protein synthesis through a 4E-BP1 and elongation factor 2 kinase pathway controlled by mTOR and uncoupled in breast cancer cells". Mol. Cell. Biol. 26 (10): 3955–65. doi:10.1128/MCB.26.10.3955-3965.2006. PMC 1489005. PMID 16648488. CS1 maint: Date and year (link)
↑ Harris TE, Chi A, Shabanowitz J, Hunt DF, Rhoads RE, Lawrence JC (Apr 2006). "mTOR-dependent stimulation of the association of eIF4G and eIF3 by insulin". EMBO J. 25 (8): 1659–68. doi:10.1038/sj.emboj.7601047. PMC 1440840. PMID 16541103. CS1 maint: Date and year (link)

Further reading

Reddy NS, Roth WW, Bragg PW, Wahba AJ (1988). "Isolation and mapping of a gene for protein synthesis initiation factor 4A and its expression during differentiation of murine erythroleukemia cells". Gene 70 (2): 231–43. doi:10.1016/0378-1119(88)90195-3. PMID 3215517.
Kukimoto I, Watanabe S, Taniguchi K, Ogata T, Yoshiike K, Kanda T (1997). "Characterization of the cloned promoter of the human initiation factor 4AI gene". Biochem. Biophys. Res. Commun. 233 (3): 844–7. doi:10.1006/bbrc.1997.6555. PMID 9168945.
Imataka H, Sonenberg N (1997). "Human eukaryotic translation initiation factor 4G (eIF4G) possesses two separate and independent binding sites for eIF4A". Mol. Cell. Biol. 17 (12): 6940–7. PMC 232551. PMID 9372926.
Gradi A, Imataka H, Svitkin YV, Rom E, Raught B, Morino S et al. (1998). "A novel functional human eukaryotic translation initiation factor 4G". Mol. Cell. Biol. 18 (1): 334–42. PMC 121501. PMID 9418880.
Craig AW, Haghighat A, Yu AT, Sonenberg N (1998). "Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation". Nature 392 (6675): 520–3. doi:10.1038/33198. PMID 9548260.
Henis-Korenblit S, Strumpf NL, Goldstaub D, Kimchi A (2000). "A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation". Mol. Cell. Biol. 20 (2): 496–506. doi:10.1128/MCB.20.2.496-506.2000. PMC 85113. PMID 10611228.
Quinn CM, Wiles AP, El-Shanawany T, Catchpole I, Alnadaf T, Ford MJ et al. (1999). "The human eukaryotic initiation factor 4AI gene (EIF4A1) contains multiple regulatory elements that direct high-level reporter gene expression in mammalian cell lines". Genomics 62 (3): 468–76. doi:10.1006/geno.1999.6031. PMID 10644445.
Cuesta R, Xi Q, Schneider RJ (2000). "Adenovirus-specific translation by displacement of kinase Mnk1 from cap-initiation complex eIF4F". EMBO J. 19 (13): 3465–74. doi:10.1093/emboj/19.13.3465. PMC 313943. PMID 10880459.
Mendell JT, Medghalchi SM, Lake RG, Noensie EN, Dietz HC (2000). "Novel Upf2p orthologues suggest a functional link between translation initiation and nonsense surveillance complexes". Mol. Cell. Biol. 20 (23): 8944–57. doi:10.1128/MCB.20.23.8944-8957.2000. PMC 86549. PMID 11073994.
Li W, Belsham GJ, Proud CG (2001). "Eukaryotic initiation factors 4A (eIF4A) and 4G (eIF4G) mutually interact in a 1:1 ratio in vivo". J. Biol. Chem. 276 (31): 29111–5. doi:10.1074/jbc.C100284200. PMID 11408474.
Du MX, Johnson RB, Sun XL, Staschke KA, Colacino J, Wang QM (2002). "Comparative characterization of two DEAD-box RNA helicases in superfamily II: human translation-initiation factor 4A and hepatitis C virus non-structural protein 3 (NS3) helicase". Biochem. J. 363 (Pt 1): 147–55. doi:10.1042/0264-6021:3630147. PMC 1222461. PMID 11903057.
Bohnsack MT, Regener K, Schwappach B, Saffrich R, Paraskeva E, Hartmann E et al. (2002). "Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm". EMBO J. 21 (22): 6205–15. doi:10.1093/emboj/cdf613. PMC 137205. PMID 12426392.
Yang HS, Cho MH, Zakowicz H, Hegamyer G, Sonenberg N, Colburn NH (2004). "A novel function of the MA-3 domains in transformation and translation suppressor Pdcd4 is essential for its binding to eukaryotic translation initiation factor 4A". Mol. Cell. Biol. 24 (9): 3894–906. doi:10.1128/MCB.24.9.3894-3906.2004. PMC 387765. PMID 15082783.
Mingot JM, Bohnsack MT, Jäkle U, Görlich D (2004). "Exportin 7 defines a novel general nuclear export pathway". EMBO J. 23 (16): 3227–36. doi:10.1038/sj.emboj.7600338. PMC 514512. PMID 15282546. Vancouver style error (help)
Hinton TM, Coldwell MJ, Carpenter GA, Morley SJ, Pain VM (2007). "Functional analysis of individual binding activities of the scaffold protein eIF4G". J. Biol. Chem. 282 (3): 1695–708. doi:10.1074/jbc.M602780200. PMID 17130132.
Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

PDB gallery

2g9n: Structure of the DEAD domain of Human eukaryotic initiation factor 4A, eIF4A