DEPTOR
| DEP domain containing MTOR-interacting protein | |||||||||||||
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| Identifiers | |||||||||||||
| Symbols | DEPTOR ; DEP.6; DEPDC6 | ||||||||||||
| External IDs | OMIM: 612974 MGI: 2146322 HomoloGene: 32551 GeneCards: DEPTOR Gene | ||||||||||||
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| RNA expression pattern | |||||||||||||
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| More reference expression data | |||||||||||||
| Orthologs | |||||||||||||
| Species | Human | Mouse | |||||||||||
| Entrez | 64798 | 97998 | |||||||||||
| Ensembl | ENSG00000155792 | ENSMUSG00000022419 | |||||||||||
| UniProt | Q8TB45 | Q570Y9 | |||||||||||
| RefSeq (mRNA) | NM_001283012 | NM_001037937 | |||||||||||
| RefSeq (protein) | NP_001269941 | NP_001033026 | |||||||||||
| Location (UCSC) | Chr 8: 120.89 – 121.06 Mb | Chr 15: 55.11 – 55.26 Mb | |||||||||||
| PubMed search | |||||||||||||
DEP domain-containing mTOR-interacting protein (DEPTOR) also known as DEP domain-containing protein 6 (DEPDC6) is a protein that in humans is encoded by the DEPTOR gene.[1]
Structure
The gene DEPTOR can be found only in vertebrates. In human, DEPTOR gene locates at chromosome 8, 8q24.12 with protein size 409 a.a.[2] Human DEPTOR contains two N-terminal DEP domains and a C-terminal PDZ domain.[3]
Function
DEPTOR is involved in mTOR signaling pathway as an inhibitor. A direct interaction between DEPTOR and mTOR has been shown.[3] Overexpression of DEPTOR downregulates the activity of mTORC1 while activates mTORC2. mTORC1 and mTORC2 can both inhibit DEPTOR through phosphorylation.[3]
Clinical cancer
Although in most cancer, mTOR pathway is constitutively activated and the expression of DEPTOR is low, one study has found that DEPTOR is overexpressed in multiple myeloma cells and is necessary for their survival.[3]
References
- ↑ "Entrez Gene: DEPDC6 DEP domain containing 6".
- ↑ "Homo sapiens - Ensembl protein families - Gene: DEPTOR (ENSG00000155792)". Ensembl genome browser 78.
- ↑ 3.0 3.1 3.2 3.3 Peterson TR, Laplante M, Thoreen CC, Sancak Y, Kang SA, Kuehl WM et al. (2009). "DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival". Cell 137 (5): 873–886. doi:10.1016/j.cell.2009.03.046. PMID 19446321.
Further reading
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Hartley JL, Temple GF, Brasch MA (2000). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
- Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
- Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S (2000). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
- Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
- Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.