Cryo-electron tomography
Cryo-electron tomography (cryo-ET or electron cryotomography) is a type of electron cryomicroscopy where tomography is used to obtain a 3D reconstruction of a sample from tilted 2D images at cryogenic temperatures. A cryoelectron tomography can be used to obtain structural details of complex cellular organizations at subnanometer resolutions.[1] The signal in cryo-electron tomograms is rather low, typically in the 0.01SNR regime. Resolution will be limited to about 50Angstrom. However, single particle analysis of cryo-electron subtomograms can improve resolution to about 15-30Angstrom. [2]
References
External links
- Explanation with multimedia
- Electron Cryotomography by Gavin E. Murphy and Grant J. Jensen
- Electron cryotomography: a new view into microbial ultrastructure by Zhuo Li and Grant J Jensen
- Example of tomography
- Software for processing Cryo Electron Tomography data