Crossover junction endodeoxyribonuclease

Crossover junction endodeoxyribonuclease
Identifiers
EC number 3.1.22.4
CAS number 99676-43-4
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Crossover junction endodeoxyribonuclease (EC 3.1.22.4, Hje endonuclease, Holliday junction endonuclease CCE1, Holliday junction resolvase, Holliday junction-cleaving endonuclease, Holliday junction-resolving endoribonuclease, RusA Holliday junction resolvase, RusA endonuclease, RuvC endonuclease, SpCCe1 Holliday junction resolvase, crossover junction endoribonuclease, cruciform-cutting endonuclease, endo X3, endonuclease RuvC, endonuclease VII, endonuclease X3, resolving enzyme CCE1) is an enzyme.[1][2][3][4][5] This enzyme catalyses the following chemical reaction

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)

The enzyme from Saccharomyces cerevisiae is not active on DNA molecules that do not contain Holliday junctions.

References

  1. ↑ Symington, L.S. and Kolodner, R. (1985). "Partial purification of an enzyme from Saccharomyces cerevisiae that cleaves Holliday junctions". Proc. Natl. Acad. Sci. USA 82: 7247–7251. doi:10.1073/pnas.82.21.7247. PMID 3903750.
  2. ↑ Shida, T., Iwasaki, H., Saito, A., Kyogoku, Y. and Shinagawa, H. (1996). "Analysis of substrate specificity of the RuvC holliday junction resolvase with synthetic Holliday junctions". J. Biol. Chem. 271: 26105–26109. doi:10.1074/jbc.271.42.26105. PMID 8824253.
  3. ↑ Shah, R., Cosstick, R. and West, S.C. (1997). "The RuvC protein dimer resolves Holliday junctions by a dual incision mechanism that involves base-specific contacts". EMBO J. 16: 1464–1472. doi:10.1093/emboj/16.6.1464. PMID 9135161.
  4. ↑ Fogg, J.M., Schofield, M.J., White, M.F. and Lilley, D.M. (1999). "Sequence and functional-group specificity for cleavage of DNA junctions by RuvC of Escherichia coli". Biochemistry 38: 11349–11358. doi:10.1021/bi990926n. PMID 10471285.
  5. ↑ Middleton, C.L., Parker, J.L., Richard, D.J., White, M.F. and Bond, C.S. (2003). "Crystallization and preliminary X-ray diffraction studies of Hje, a Holliday junction resolving enzyme from Sulfolobus solfataricus". Acta Crystallogr. D Biol. Crystallogr. 59: 171–173. doi:10.1107/s0907444902019546. PMID 12499561.

External links