Covalitoxin-II

Covalitoxin-II is a peptide toxin that is produced by the spider Coremiocnemis validus. It can induce excitatory, non-lethal behavioral symptoms like quivering and jerking in crickets.

Source

Covalitoxin-II (Cvtx-II) is a toxin in the venom of Coremiocnemis validus (Singapore or Blue femur tarantula).[1][2] This spider lives in South East Asian tropical forests.[1]

Chemistry

Structure

The toxin consists of 31 amino acids (ACSRAGENCYKSGRCCDGLYCKAYVVTCYKP). This sequence forms a peptide with a molecular weight of 3.4 kDa. It has six cysteine residues which form three disulphide bonds, between the specific amino acid locations 2&16, 19&21 and 15&28.[2] There is an equal distribution of hydrophilic, hydrophobic and neutral amino acids. Near the C-terminus there is a positively charged surface formed by conserved basic residues, which is thought to interact with an ion channel.[1][3] The inhibitor cystine knot motif that is formed by the cysteine residues shows analogy to other spider toxins and can also be found in ω-conotoxins, which are present in the venom of cone snails. It has low homology with two peptides:

Target and mode of action

The specific target and mode of action of Cvtx-II are not yet clear. Based on its effect on behavior, it has been speculated that Cvtx-II targets sodium channel inactivation, analogous to some excitotoxins.[1]

Toxicity

Cvtx-II induces the following non-lethal symptoms in crickets:

Experiments have shown that these symptoms are not in present in cockroaches or mice after Cvtx-II injections. Therefore, Cvtx-II is thought to be an insect species-specific neurotoxic peptide. A dose of 0.2 µmol/g was necessary to immobilize or inactivate 50% of the insects (ID50). After 40–60 minutes, the immobilizing effects disappear.[1]

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Balaji RA, Sasaki T, Gopalakrishnakone P et al.; Purification, structure determination and synthesis of covalitoxin-II, a short insect-specific neurotoxic peptide from the venom of the Coremiocnemis validus (Singapore tarantula); FEBS Lett. 2000 Jun 2;474(2-3):208-12. Balaji, R. A.; Sasaki, T.; Gopalakrishnakone, P.; Sato, K.; Kini, R. M.; Bay, B. H. (2000). "Purification, structure determination and synthesis of covalitoxin-II, a short insect-specific neurotoxic peptide from the venom of the Coremiocnemis validus (Singapore tarantula)". FEBS letters 474 (2–3): 208–212. doi:10.1016/S0014-5793(00)01593-3. PMID 10838086.
  2. ↑ 2.0 2.1 UniProt, UniProt Knowledge Base; P82601 (TXVL2_CORVA); Publicated December 1, 2000; Last modified October 3, 2012; Date of retrieval October 16, 2012;
  3. ↑ Grishin E; Polypeptide neurotoxins from spider venoms; Eur J Biochem. 1999 Sep;264(2):276-80. Grishin, E. (1999). "Polypeptide neurotoxins from spider venoms". European journal of biochemistry / FEBS 264 (2): 276–280. doi:10.1046/j.1432-1327.1999.00622.x. PMID 10491071.