Carboxyfluorescein

Carboxyfluorescein
Names
Other names
6-FAM
Identifiers
3301-79-9 Yes
ChEBI CHEBI:39073 Yes
ChemSpider 69262 Yes
Jmol-3D images Image
Properties
Molecular formula
 C21H12O7
Molar mass 376.32 g·mol−1
Except where noted otherwise, data is given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
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Infobox references

Carboxyfluorescein is either of two fluorescent dyes with an excitation and emission of 492/517 nm, respectively. A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. They are commonly used as a tracer agents. The dyes are membrane-impermeant and can be loaded into cells by microinjection or scrape loading.[1] It can be incorporated into liposomes, and allow for the tracking of liposomes as they pass through the body. In addition, carboxyfluorescein has been used to track division of cells.[2]

Popular derivatives for cell tracing purposes are Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and Carboxyfluorescein succinimidyl ester (CFSE).

See also

References

  1. ↑ Molecular Imaging Products Company (2005-08-26). "5-(and-6)-Carboxyfluorescein (5-(and-6)- FAM,mixed isomer) 100mg". Retrieved 2006-08-26.
  2. ↑ Parish, Christopher (December 1999). "Fluorescent dyes for lymphocyte migration and proliferation studies". Immunology and Cell Biology. Blackwell Synergy. Retrieved 2006-08-26.