CUL3

Cullin 3

PDB rendering based on 1iuy.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
4AP2, 4APF, 4EOZ, 4HXI

Identifiers

Symbols

CUL3 ; CUL-3; PHA2E

External IDs

OMIM: 603136 MGI: 1347360 HomoloGene: 2661 GeneCards: CUL3 Gene

Gene ontology
Molecular function	• ubiquitin-protein transferase activity • protein binding • cyclin binding • POZ domain binding • ubiquitin protein ligase binding
Cellular component	• Golgi membrane • nucleus • polar microtubule • membrane • Cul3-RING ubiquitin ligase complex • extracellular vesicular exosome
Biological process	• G1/S transition of mitotic cell cycle • mitotic anaphase • protein polyubiquitination • cytokinesis • trophectodermal cellular morphogenesis • protein monoubiquitination • ER to Golgi vesicle-mediated transport • cell cycle arrest • integrin-mediated signaling pathway • gastrulation • cyclin catabolic process • positive regulation of cell proliferation • Wnt signaling pathway • cell migration • protein ubiquitination • stem cell division • positive regulation of cytokinesis • negative regulation of Rho protein signal transduction • embryonic cleavage • stress fiber assembly • proteasome-mediated ubiquitin-dependent protein catabolic process • COPII vesicle coating • anaphase • intrinsic apoptotic signaling pathway
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 2:
225.33 – 225.45 Mb

Chr 1:
80.26 – 80.34 Mb

PubMed search

Cullin-3 is a protein that in humans is encoded by the CUL3 gene.^[1]^[2]^[3]

Interactions

CUL3 has been shown to interact with KLHL12,^[4] CAND1,^[5] KEAP1,^[6]^[7] DCUN1D1^[8] and Cyclin E1.^[9]

References

↑ Kipreos ET, Lander LE, Wing JP, He WW, Hedgecock EM (Aug 1996). "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family". Cell 85 (6): 829–39. doi:10.1016/S0092-8674(00)81267-2. PMID 8681378.
↑ Wimuttisuk W, Singer JD (Feb 2007). "The Cullin3 Ubiquitin Ligase Functions as a Nedd8-bound Heterodimer". Mol Biol Cell 18 (3): 899–909. doi:10.1091/mbc.E06-06-0542. PMC 1805106. PMID 17192413.
↑ "Entrez Gene: CUL3 cullin 3".
↑ Rondou, Pieter; Haegeman Guy; Vanhoenacker Peter; Van Craenenbroeck Kathleen (Apr 2008). "BTB Protein KLHL12 Targets the Dopamine D4 Receptor for Ubiquitination by a Cul3-based E3 Ligase". J. Biol. Chem. (United States) 283 (17): 11083–96. doi:10.1074/jbc.M708473200. ISSN 0021-9258. PMC 2431063. PMID 18303015.
↑ Min, Kyoeng-Woo; Hwang Ji-Won; Lee Jong-Sik; Park Yoon; Tamura Taka-aki; Yoon Jong-Bok (May 2003). "TIP120A associates with cullins and modulates ubiquitin ligase activity". J. Biol. Chem. (United States) 278 (18): 15905–10. doi:10.1074/jbc.M213070200. ISSN 0021-9258. PMID 12609982.
↑ Wang, Xiao-Jun; Sun Zheng; Chen Weimin; Li Yanjie; Villeneuve Nicole F; Zhang Donna D (Aug 2008). "Activation of Nrf2 by arsenite and monomethylarsonous acid is independent of Keap1-C151: enhanced Keap1-Cul3 interaction". Toxicol. Appl. Pharmacol. (United States) 230 (3): 383–9. doi:10.1016/j.taap.2008.03.003. ISSN 0041-008X. PMC 2610481. PMID 18417180.
↑ Rachakonda, Girish; Xiong Ying; Sekhar Konjeti R; Stamer Sheryl L; Liebler Daniel C; Freeman Michael L (Mar 2008). "Covalent modification at Cys151 dissociates the electrophile sensor Keap1 from the ubiquitin ligase CUL3". Chem. Res. Toxicol. (United States) 21 (3): 705–10. doi:10.1021/tx700302s. ISSN 0893-228X. PMID 18251510.
↑ Kim, Alexander Y; Bommeljé Claire C, Lee Benjamin E, Yonekawa Yoshihiro, Choi Lydia, Morris Luc G, Huang Guochang, Kaufman Andrew, Ryan Russel J H, Hao Bing, Ramanathan Y, Singh Bhuvanesh (Nov 2008). "SCCRO (DCUN1D1) Is an Essential Component of the E3 Complex for Neddylation". J. Biol. Chem. (United States) 283 (48): 33211–20. doi:10.1074/jbc.M804440200. ISSN 0021-9258. PMC 2586271. PMID 18826954.
↑ Singer, J D; Gurian-West M; Clurman B; Roberts J M (Sep 1999). "Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells". Genes Dev. (UNITED STATES) 13 (18): 2375–87. doi:10.1101/gad.13.18.2375. ISSN 0890-9369. PMC 317026. PMID 10500095.

Andersson B, Wentland MA, Ricafrente JY et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
Yu W, Andersson B, Worley KC et al. (1997). "Large-Scale Concatenation cDNA Sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
Michel JJ, Xiong Y (1998). "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A". Cell Growth Differ. 9 (6): 435–49. PMID 9663463.
Du M, Sansores-Garcia L, Zu Z, Wu KK (1998). "Cloning and expression analysis of a novel salicylate suppressible gene, Hs-CUL-3, a member of cullin/Cdc53 family". J. Biol. Chem. 273 (38): 24289–92. doi:10.1074/jbc.273.38.24289. PMID 9733711.
Ishikawa K, Nagase T, Suyama M et al. (1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (3): 169–76. doi:10.1093/dnares/5.3.169. PMID 9734811.
Ohta T, Michel JJ, Schottelius AJ, Xiong Y (1999). "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity". Mol. Cell 3 (4): 535–41. doi:10.1016/S1097-2765(00)80482-7. PMID 10230407.
Singer JD, Gurian-West M, Clurman B, Roberts JM (1999). "Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells". Genes Dev. 13 (18): 2375–87. doi:10.1101/gad.13.18.2375. PMC 317026. PMID 10500095.
Hori T, Osaka F, Chiba T et al. (2000). "Covalent modification of all members of human cullin family proteins by NEDD8". Oncogene 18 (48): 6829–34. doi:10.1038/sj.onc.1203093. PMID 10597293.
Maeda I, Ohta T, Koizumi H, Fukuda M (2001). "In vitro ubiquitination of cyclin D1 by ROC1-CUL1 and ROC1-CUL3". FEBS Lett. 494 (3): 181–5. doi:10.1016/S0014-5793(01)02343-2. PMID 11311237.
Lyapina S, Cope G, Shevchenko A et al. (2001). "Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome". Science 292 (5520): 1382–5. doi:10.1126/science.1059780. PMID 11337588.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Min KW, Hwang JW, Lee JS et al. (2003). "TIP120A associates with cullins and modulates ubiquitin ligase activity". J. Biol. Chem. 278 (18): 15905–10. doi:10.1074/jbc.M213070200. PMID 12609982.
Kobayashi A, Kang MI, Okawa H et al. (2004). "Oxidative Stress Sensor Keap1 Functions as an Adaptor for Cul3-Based E3 Ligase To Regulate Proteasomal Degradation of Nrf2". Mol. Cell. Biol. 24 (16): 7130–9. doi:10.1128/MCB.24.16.7130-7139.2004. PMC 479737. PMID 15282312.
Beausoleil SA, Jedrychowski M, Schwartz D et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
Gerhard DS, Wagner L, Feingold EA et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Rush J, Moritz A, Lee KA et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455.
Furukawa M, Xiong Y (2005). "BTB Protein Keap1 Targets Antioxidant Transcription Factor Nrf2 for Ubiquitination by the Cullin 3-Roc1 Ligase". Mol. Cell. Biol. 25 (1): 162–71. doi:10.1128/MCB.25.1.162-171.2005. PMC 538799. PMID 15601839.
Hernández-Muñoz I, Lund AH, van der Stoop P et al. (2005). "Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase". Proc. Natl. Acad. Sci. U.S.A. 102 (21): 7635–40. doi:10.1073/pnas.0408918102. PMC 1140410. PMID 15897469.
Lu L, Zhou ZM, Huang XY et al. (2005). "Identification and characterization of cul-3b, a novel hominine CUL-3 transcript variant". Asian J. Androl. 7 (2): 205–11. doi:10.1111/j.1745-7262.2005.00024.x. PMID 15897978.

PDB gallery

1iuy: Solution structure of the cullin-3 homologue

CUL3

Interactions

References

Further reading