CPSF6
| Cleavage and polyadenylation specific factor 6, 68kDa | |||||||||||||
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 Rendering based on PDB 3P5T. | |||||||||||||
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| Identifiers | |||||||||||||
| Symbols | CPSF6 ; CFIM; CFIM68; HPBRII-4; HPBRII-7 | ||||||||||||
| External IDs | OMIM: 604979 MGI: 1913948 HomoloGene: 134126 GeneCards: CPSF6 Gene | ||||||||||||
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| RNA expression pattern | |||||||||||||
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| Orthologs | |||||||||||||
| Species | Human | Mouse | |||||||||||
| Entrez | 11052 | 432508 | |||||||||||
| Ensembl | ENSG00000111605 | ENSMUSG00000055531 | |||||||||||
| UniProt | Q16630 | Q6NVF9 | |||||||||||
| RefSeq (mRNA) | NM_001300947 | NM_001013391 | |||||||||||
| RefSeq (protein) | NP_001287876 | NP_001013409 | |||||||||||
| Location (UCSC) | Chr 12: 69.63 – 69.67 Mb | Chr 10: 117.34 – 117.38 Mb | |||||||||||
| PubMed search | |||||||||||||
Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene.[1][2][3]
The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitement of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins.[3]
Interactions
CPSF6 has been shown to interact with WWP1[4] and PLSCR1.[4]
References
- ↑ Ruegsegger U, Blank D, Keller W (July 1998). "Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits". Mol Cell 1 (2): 243–53. doi:10.1016/S1097-2765(00)80025-8. PMID 9659921.
- ↑ Cardinale S, Cisterna B, Bonetti P, Aringhieri C, Biggiogera M, Barabino SM (March 2007). "Subnuclear localization and dynamics of the Pre-mRNA 3' end processing factor mammalian cleavage factor I 68-kDa subunit". Mol Biol Cell 18 (4): 1282–92. doi:10.1091/mbc.E06-09-0846. PMC 1838998. PMID 17267687.
- ↑ 3.0 3.1 "Entrez Gene: CPSF6 cleavage and polyadenylation specific factor 6, 68kDa".
- ↑ 4.0 4.1 Rual, Jean-François; Venkatesan Kavitha, Hao Tong, Hirozane-Kishikawa Tomoko, Dricot Amélie, Li Ning, Berriz Gabriel F, Gibbons Francis D, Dreze Matija, Ayivi-Guedehoussou Nono, Klitgord Niels, Simon Christophe, Boxem Mike, Milstein Stuart, Rosenberg Jennifer, Goldberg Debra S, Zhang Lan V, Wong Sharyl L, Franklin Giovanni, Li Siming, Albala Joanna S, Lim Janghoo, Fraughton Carlene, Llamosas Estelle, Cevik Sebiha, Bex Camille, Lamesch Philippe, Sikorski Robert S, Vandenhaute Jean, Zoghbi Huda Y, Smolyar Alex, Bosak Stephanie, Sequerra Reynaldo, Doucette-Stamm Lynn, Cusick Michael E, Hill David E, Roth Frederick P, Vidal Marc (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature (England) 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Further reading
- Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Rüegsegger U, Beyer K, Keller W (1996). "Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors". J. Biol. Chem. 271 (11): 6107–13. doi:10.1074/jbc.271.11.6107. PMID 8626397.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- de Vries H, Rüegsegger U, Hübner W et al. (2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". EMBO J. 19 (21): 5895–904. doi:10.1093/emboj/19.21.5895. PMC 305781. PMID 11060040.
- Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Awasthi S, Alwine JC (2003). "Association of polyadenylation cleavage factor I with U1 snRNP". RNA 9 (11): 1400–9. doi:10.1261/rna.5104603. PMC 1287061. PMID 14561889.
- Brown KM, Gilmartin GM (2004). "A mechanism for the regulation of pre-mRNA 3' processing by human cleavage factor Im". Mol. Cell 12 (6): 1467–76. doi:10.1016/S1097-2765(03)00453-2. PMID 14690600.
- Dettwiler S, Aringhieri C, Cardinale S et al. (2005). "Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization". J. Biol. Chem. 279 (34): 35788–97. doi:10.1074/jbc.M403927200. PMID 15169763.
- Gerhard DS, Wagner L, Feingold EA et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Venkataraman K, Brown KM, Gilmartin GM (2005). "Analysis of a noncanonical poly(A) site reveals a tripartite mechanism for vertebrate poly(A) site recognition". Genes Dev. 19 (11): 1315–27. doi:10.1101/gad.1298605. PMC 1142555. PMID 15937220.
- Rual JF, Venkatesan K, Hao T et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
- Kimura K, Wakamatsu A, Suzuki Y et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
- Millevoi S, Loulergue C, Dettwiler S et al. (2006). "An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries". EMBO J. 25 (20): 4854–64. doi:10.1038/sj.emboj.7601331. PMC 1618107. PMID 17024186.
- Ewing RM, Chu P, Elisma F et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.