CDC45-related protein
| Cell division cycle 45 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||||||
| Symbols | CDC45 ; CDC45L; CDC45L2; PORC-PI-1 | ||||||||||||
| External IDs | OMIM: 603465 MGI: 1338073 HomoloGene: 2616 ChEMBL: 3040 GeneCards: CDC45 Gene | ||||||||||||
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| RNA expression pattern | |||||||||||||
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| More reference expression data | |||||||||||||
| Orthologs | |||||||||||||
| Species | Human | Mouse | |||||||||||
| Entrez | 8318 | 12544 | |||||||||||
| Ensembl | ENSG00000093009 | ENSMUSG00000000028 | |||||||||||
| UniProt | O75419 | Q9Z1X9 | |||||||||||
| RefSeq (mRNA) | NM_001178010 | NM_001161623 | |||||||||||
| RefSeq (protein) | NP_001171481 | NP_001155095 | |||||||||||
| Location (UCSC) | Chr 22: 19.47 – 19.51 Mb | Chr 16: 18.78 – 18.81 Mb | |||||||||||
| PubMed search | |||||||||||||
CDC45 is a protein that in humans is encoded by the CDC45L gene.[1][2][3][4]
Function
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.[4]
Interactions
CDC45-related protein has been shown to interact with:
References
- ↑ Saha P, Thome KC, Yamaguchi R, Hou Z, Weremowicz S, Dutta A (Aug 1998). "The human homolog of Saccharomyces cerevisiae CDC45". J. Biol. Chem. 273 (29): 18205–9. doi:10.1074/jbc.273.29.18205. PMID 9660782.
- ↑ McKie JM, Wadey RB, Sutherland HF, Taylor CL, Scambler PJ (Sep 1998). "Direct selection of conserved cDNAs from the DiGeorge critical region: isolation of a novel CDC45-like gene". Genome Res. 8 (8): 834–41. doi:10.1101/gr.8.8.834. PMC 310757. PMID 9724329.
- ↑ Pollok S, Bauerschmidt C, Sänger J, Nasheuer HP, Grosse F (Jul 2007). "Human Cdc45 is a proliferation-associated antigen". FEBS J. 274 (14): 3669–84. doi:10.1111/j.1742-4658.2007.05900.x. PMID 17608804. Vancouver style error (help)
- ↑ 4.0 4.1 "Entrez Gene: CDC45L CDC45 cell division cycle 45-like (S. cerevisiae)".
- ↑ 5.0 5.1 5.2 5.3 5.4 Kneissl M, Pütter V, Szalay AA, Grummt F (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612. Vancouver style error (help)
- ↑ Kukimoto I, Igaki H, Kanda T (Nov 1999). "Human CDC45 protein binds to minichromosome maintenance 7 protein and the p70 subunit of DNA polymerase alpha". Eur. J. Biochem. 265 (3): 936–43. doi:10.1046/j.1432-1327.1999.00791.x. PMID 10518787.
Further reading
- Leatherwood J (1998). "Emerging mechanisms of eukaryotic DNA replication initiation". Curr. Opin. Cell Biol. 10 (6): 742–8. doi:10.1016/S0955-0674(98)80117-8. PMID 9914182.
- Mimura S, Takisawa H (1998). "Xenopus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-phase Cdk". EMBO J. 17 (19): 5699–707. doi:10.1093/emboj/17.19.5699. PMC 1170898. PMID 9755170.
- Shaikh TH, Gottlieb S, Sellinger B, Chen F, Roe BA, Oakey RJ et al. (1999). "Characterization of CDC45L: a gene in the 22q11.2 deletion region expressed during murine and human development". Mamm. Genome 10 (3): 322–6. doi:10.1007/s003359900996. PMID 10051334.
- Kukimoto I, Igaki H, Kanda T (1999). "Human CDC45 protein binds to minichromosome maintenance 7 protein and the p70 subunit of DNA polymerase alpha". Eur. J. Biochem. 265 (3): 936–43. doi:10.1046/j.1432-1327.1999.00791.x. PMID 10518787.
- Kneissl M, Pütter V, Szalay AA, Grummt F (2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/S0022-2836(03)00079-2. PMID 12614612. Vancouver style error (help)
- Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J et al. (2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
- Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau AY et al. (2004). "Self-assembling protein microarrays". Science 305 (5680): 86–90. doi:10.1126/science.1097639. PMID 15232106.
- Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
- Liu P, Barkley LR, Day T, Bi X, Slater DM, Alexandrow MG et al. (2006). "The Chk1-mediated S-phase checkpoint targets initiation factor Cdc45 via a Cdc25A/Cdk2-independent mechanism". J. Biol. Chem. 281 (41): 30631–44. doi:10.1074/jbc.M602982200. PMID 16912045.
- Masai H, Taniyama C, Ogino K, Matsui E, Kakusho N, Matsumoto S et al. (2006). "Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatin". J. Biol. Chem. 281 (51): 39249–61. doi:10.1074/jbc.M608935200. PMID 17046832.
- Boskovic J, Coloma J, Aparicio T, Zhou M, Robinson CV, Méndez J et al. (2007). "Molecular architecture of the human GINS complex". EMBO Rep. 8 (7): 678–84. doi:10.1038/sj.embor.7401002. PMC 1905900. PMID 17557111. Vancouver style error (help)
- Bauerschmidt C, Pollok S, Kremmer E, Nasheuer HP, Grosse F (2007). "Interactions of human Cdc45 with the Mcm2-7 complex, the GINS complex, and DNA polymerases delta and epsilon during S phase". Genes Cells 12 (6): 745–58. doi:10.1111/j.1365-2443.2007.01090.x. PMID 17573775.
- Pollok S, Grosse F (2007). "Cdc45 degradation during differentiation and apoptosis". Biochem. Biophys. Res. Commun. 362 (4): 910–5. doi:10.1016/j.bbrc.2007.08.069. PMID 17767920.