CAPN2

Calpain 2, (m/II) large subunit

PDB rendering based on 1df0.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
SymbolsCAPN2 ; CANP2; CANPL2; CANPml; mCANP
External IDsOMIM: 114230 MGI: 88264 HomoloGene: 1326 IUPHAR: 2337 ChEMBL: 2382 GeneCards: CAPN2 Gene
EC number3.4.22.53
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez82412334
EnsemblENSG00000162909ENSMUSG00000026509
UniProtP17655O08529
RefSeq (mRNA)NM_001146068NM_009794
RefSeq (protein)NP_001139540NP_033924
Location (UCSC)Chr 1:
223.89 – 223.96 Mb		Chr 1:
182.47 – 182.52 Mb
PubMed search

Calpain-2 catalytic subunit is a protein that in humans is encoded by the CAPN2 gene.[1][2]

Function

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported.[3]

Interactions

CAPN2 has been shown to interact with Bcl-2.[4]

References

  1. ↑ Imajoh S, Aoki K, Ohno S, Emori Y, Kawasaki H, Sugihara H et al. (May 1989). "Molecular cloning of the cDNA for the large subunit of the high-Ca2+-requiring form of human Ca2+-activated neutral protease". Biochemistry 27 (21): 8122–8. doi:10.1021/bi00421a022. PMID 2852952. Check date values in: |year= / |date= mismatch (help)
  2. ↑ Hata A, Ohno S, Akita Y, Suzuki K (May 1989). "Tandemly reiterated negative enhancer-like elements regulate transcription of a human gene for the large subunit of calcium-dependent protease". J. Biol. Chem. 264 (11): 6404–11. PMID 2539381.
  3. ↑ "Entrez Gene: CAPN2 calpain 2, (m/II) large subunit".
  4. ↑ Gil-Parrado S, Fernández-Montalván A, Assfalg-Machleidt I, Popp O, Bestvater F, Holloschi A et al. (Jul 2002). "Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members". J. Biol. Chem. 277 (30): 27217–26. doi:10.1074/jbc.M202945200. PMID 12000759.

Further reading

  • Suzuki K, Sorimachi H, Yoshizawa T, Kinbara K, Ishiura S (1995). "Calpain: novel family members, activation, and physiologic function". Biol. Chem. Hoppe-Seyler 376 (9): 523–9. PMID 8561910.
  • Cohen GM (1997). "Caspases: the executioners of apoptosis". Biochem. J. 326 ( Pt 1) (Pt 1): 1–16. PMC 1218630. PMID 9337844.
  • Reverter D, Sorimachi H, Bode W (2001). "The structure of calcium-free human m-calpain: implications for calcium activation and function". Trends Cardiovasc. Med. 11 (6): 222–9. doi:10.1016/S1050-1738(01)00112-8. PMID 11673052.
  • Kopp S (1976). "Reproducibility of response to a questionnaire on symptoms of masticatory dysfunction". Community Dent Oral Epidemiol 4 (5): 205–9. doi:10.1111/j.1600-0528.1976.tb00985.x. PMID 1067155.
  • Adachi Y, Kitahara-Ozawa A, Sugamura K, Lee WJ, Yodoi J, Maki M et al. (1992). "Expression of calpain II gene in human hematopoietic system cells infected with human T-cell leukemia virus type I". J. Biol. Chem. 267 (27): 19373–8. PMID 1527057.
  • Ohno S, Minoshima S, Kudoh J, Fukuyama R, Shimizu Y, Ohmi-Imajoh S et al. (1990). "Four genes for the calpain family locate on four distinct human chromosomes". Cytogenet. Cell Genet. 53 (4): 225–9. doi:10.1159/000132937. PMID 2209092.
  • Ishiguro H, Higashiyama S, Namikawa C, Kunimatsu M, Takano E, Tanaka K et al. (1987). "Interaction of human calpains I and II with high molecular weight and low molecular weight kininogens and their heavy chain: mechanism of interaction and the role of divalent cations". Biochemistry 26 (10): 2863–70. doi:10.1021/bi00384a030. PMID 3038169.
  • Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N, Armstrong RC, Wang L et al. (1996). "The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32". J. Biol. Chem. 271 (43): 27099–106. doi:10.1074/jbc.271.43.27099. PMID 8900201.
  • Corasaniti MT, Navarra M, Catani MV, Melino G, Nisticò G, Finazzi-Agrò A (1996). "NMDA and HIV-1 coat protein, GP120, produce necrotic but not apoptotic cell death in human CHP100 neuroblastoma cultures via a mechanism involving calpain". Biochem. Biophys. Res. Commun. 229 (1): 299–304. doi:10.1006/bbrc.1996.1796. PMID 8954122.
  • Fujitani K, Kambayashi J, Sakon M, Ohmi SI, Kawashima S, Yukawa M et al. (1997). "Identification of mu-, m-calpains and calpastatin and capture of mu-calpain activation in endothelial cells". J. Cell. Biochem. 66 (2): 197–209. doi:10.1002/(SICI)1097-4644(19970801)66:2<197::AID-JCB7>3.0.CO;2-L. PMID 9213221.
  • Rock MT, Brooks WH, Roszman TL (1997). "Calcium-dependent signaling pathways in T cells. Potential role of calpain, protein tyrosine phosphatase 1b, and p130Cas in integrin-mediated signaling events". J. Biol. Chem. 272 (52): 33377–83. doi:10.1074/jbc.272.52.33377. PMID 9407132.
  • Ueyama H, Kumamoto T, Fujimoto S, Murakami T, Tsuda T (1998). "Expression of three calpain isoform genes in human skeletal muscles". J. Neurol. Sci. 155 (2): 163–9. doi:10.1016/S0022-510X(97)00309-2. PMID 9562261.
  • Strobl S, Fernandez-Catalan C, Braun M, Huber R, Masumoto H, Nakagawa K et al. (2000). "The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium". Proc. Natl. Acad. Sci. U.S.A. 97 (2): 588–92. doi:10.1073/pnas.97.2.588. PMC 15374. PMID 10639123.
  • Masumoto H, Nakagawa K, Irie S, Sorimachi H, Suzuki K, Bourenkov GP et al. (2000). "Crystallization and preliminary X-ray analysis of recombinant full-length human m-calpain". Acta Crystallogr. D Biol. Crystallogr. 56 (Pt 1): 73–5. doi:10.1107/S0907444999013748. PMID 10666632.
  • Chua BT, Guo K, Li P (2000). "Direct cleavage by the calcium-activated protease calpain can lead to inactivation of caspases". J. Biol. Chem. 275 (7): 5131–5. doi:10.1074/jbc.275.7.5131. PMID 10671558.
  • Lee MS, Kwon YT, Li M, Peng J, Friedlander RM, Tsai LH (2000). "Neurotoxicity induces cleavage of p35 to p25 by calpain". Nature 405 (6784): 360–4. doi:10.1038/35012636. PMID 10830966.

External links