Bismarck brown Y

Bismarck brown Y

Names
Other names Bismarck brown Manchester brown Phenylene brown Basic Brown 1 C.I. 21000 Vesuvine BA
Identifiers
CAS Registry Number	8005-77-4 N
ChemSpider	13374 N
InChI InChI=1S/C18H18N8/c19-11-4-6-17(15(21)8-11)25-23-13-2-1-3-14(10-13)24-26-18-7-5-12(20)9-16(18)22/h1-10H,19-22H2/b25-23+,26-24+ N Key: BDFZFGDTHFGWRQ-OGGGYYITSA-N N InChI=1/C18H18N8/c19-11-4-6-17(15(21)8-11)25-23-13-2-1-3-14(10-13)24-26-18-7-5-12(20)9-16(18)22/h1-10H,19-22H2/b25-23+,26-24+ Key: BDFZFGDTHFGWRQ-OGGGYYITBR
Jmol-3D images	Image Image
PubChem	13981
SMILES CC1=C(C=C(C=C1)N=NC2=C(C=C(C(=C2)C)N)N)N=NC3=C(C=C(C(=C3)C)N)N N(=N/c1ccc(cc1N)N)\c3cccc(/N=N/c2ccc(N)cc2N)c3
Properties
Molecular formula	C21H24N8
Molar mass	388.47 g·mol−1
Except where noted otherwise, data is given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
N verify (what is: Y/N?)
Infobox references

Bismarck brown Y is a diazo dye. It is used in histology for staining tissues. It stains acid mucins to yellow color. It can be used with live cells. It is also used to stain cartilage in bone specimens, as one of Kasten's Schiff-type reagents in the periodic acid-Schiff stain to stain cellulose, and in Feulgen stain to stain DNA.

It was more common in the past; today it is partially replaced by other stains.

Bismarck brown Y is a constituent of Papanicolaou stains.

It can also be used as a counterstain for Victoria blue R for staining of acid-fast microorganisms.

References

Lillie, RD (1977) Conn's Biological Stains, 9th ed. Baltimore: Williams & Wilkins, p. 145-146.