Asparagine
Names | |
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IUPAC name
Asparagine | |
Other names
2-Amino-3-carbamoylpropanoic acid | |
Identifiers | |
70-47-3 | |
ChEBI | CHEBI:17196 |
ChEMBL | ChEMBL58832 |
ChemSpider | 6031 |
DrugBank | DB03943 |
EC-number | 200-735-9 |
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Jmol-3D images | Image Image |
KEGG | C00152 |
PubChem | 236 |
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UNII | 7NG0A2TUHQ |
Properties | |
Molecular formula |
C4H8N2O3 |
Molar mass | 132.12 g·mol−1 |
Appearance | white crystals |
Density | 1.543 g/cm3 |
Melting point | 234 °C (453 °F; 507 K) |
Boiling point | 438 °C (820 °F; 711 K) |
2.94 g/100 mL | |
Solubility | soluble in acid, alkali negligible in methanol, ethanol, ether, benzene |
log P | -3.82 |
Acidity (pKa) | 2.02 (carboxyl), 8.80 (amino)[1] |
Structure | |
Crystal structure | orthorhomic |
Thermochemistry | |
Std enthalpy of formation (ΔfH |
-789.4 kJ/mol |
Hazards | |
MSDS | External MSDS |
NFPA 704 | |
Flash point | 219 °C (426 °F; 492 K) |
Supplementary data page | |
Refractive index (n), Dielectric constant (εr), etc. | |
Thermodynamic data |
Phase behaviour solid–liquid–gas |
UV, IR, NMR, MS | |
Except where noted otherwise, data is given for materials in their standard state (at 25 °C (77 °F), 100 kPa) | |
verify (what is: / ?) | |
Infobox references | |
Asparagine (abbreviated as Asn or N) is one of the 20 most-common natural amino acids on Earth. It has carboxamide as the side-chain's functional group. It is not an essential amino acid. Its codons are AAU and AAC.[2]
A reaction between asparagine and reducing sugars or reactive carbonyls produces acrylamide (acrylic amide) in food when heated to sufficient temperature. These products occur in baked goods such as French fries, potato chips, and toasted bread.
History
Asparagine was first isolated in 1806, under a crystalline form, by French chemists Louis Nicolas Vauquelin and Pierre Jean Robiquet (then a young assistant) from asparagus juice,[3][4] in which it is abundant — hence, the name they chose for that new compound — becoming the first amino acid to be isolated.
A few years later, in 1809, Pierre Jean Robiquet again identified, this time from liquorice root, a substance with properties he qualified as very similar to those of asparagine, that Plisson in 1828 identified as asparagine itself.[5]
Structural function in proteins
Since the asparagine side-chain can form hydrogen bond interactions with the peptide backbone, asparagine residues are often found near the beginning and the end of alpha-helices, and in turn motifs in beta sheets. Its role can be thought as "capping" the hydrogen bond interactions that would otherwise be satisfied by the polypeptide backbone. Glutamines, with an extra methylene group, have more conformational entropy and thus are less useful in this regard.
Asparagine also provides key sites for N-linked glycosylation, modification of the protein chain with the addition of carbohydrate chains. Typically, a carbohydrate tree can solely be added to an asparagine residue if the latter is flanked on the C side by X-serine or X-threonine, where X is any amino acid with the exception of proline.[6]
Sources
Dietary sources
Asparagine is not essential for humans, which means that it can be synthesized from central metabolic pathway intermediates and is not required in the diet.
Asparagine is found in:
- Animal sources: dairy, whey, beef, poultry, eggs, fish, lactalbumin, seafood
- Plant sources: asparagus, potatoes, legumes, nuts, seeds, soy, whole grains
Biosynthesis
The precursor to asparagine is oxaloacetate. Oxaloacetate is converted to aspartate using a transaminase enzyme. The enzyme transfers the amino group from glutamate to oxaloacetate producing α-ketoglutarate and aspartate. The enzyme asparagine synthetase produces asparagine, AMP, glutamate, and pyrophosphate from aspartate, glutamine, and ATP. In the asparagine synthetase reaction, ATP is used to activate aspartate, forming β-aspartyl-AMP. Glutamine donates an ammonium group, which reacts with β-aspartyl-AMP to form asparagine and free AMP.
Degradation
Asparagine usually enters the citric acid cycle in humans as malate. In bacteria, the degradation of asparagine leads to the production of oxaloacetate which is the molecule which combines with citrate in the citric acid cycle (Kreb's cycle). Asparagine is hydrolyzed to aspartate by asparaginase. Aspartate then undergoes transamination to form glutamate and oxaloacetate from alpha-ketogluterate.
Function
Asparagine is required for development and function of the brain. It also plays an important role in the synthesis of ammonia.
The addition of N-acetylglucosamine to asparagine is performed by oligosaccharyltransferase enzymes in the endoplasmic reticulum.[7] This glycosylation is important both for protein structure[8] and protein function.[9]
Betaine structure
External links
References
- ↑ R. M. C. Dawson, Daphne Elliott, W. H. Elliott and K. M. Jones. Clarendon, ed. (1959). Data for Biochemical Research. Oxford: Clarendon Press. OCLC 644267041.
- ↑ "Nomenclature and symbolism for amino acids and peptides (IUPAC-IUB Recommendations 1983)", Pure Appl. Chem. 56 (5), 1984: 595–624, doi:10.1351/pac198456050595.
- ↑ Vauquelin LN, Robiquet PJ (1806). "La découverte d'un nouveau principe végétal dans le suc des asperges". Annales de Chimie 57: 88–93.
- ↑ R.H.A. Plimmer (1912) [1908]. R.H.A. Plimmer & F.G. Hopkins, ed. The chemical composition of the proteins. Monographs on biochemistry. Part I. Analysis (2nd ed.). London: Longmans, Green and Co. p. 112. Retrieved January 18, 2010.
- ↑ Harvey Wickes Felter, M.D., and John Uri Lloyd, Phr. M., Ph. D. (1898). "Glycyrrhiza (U. S. P.)—Glycyrrhiza". King's American Dispensatory. Henriette's Herbal Homepage.
- ↑ Brooker, Robert; Widmaier, Eric; Graham, Linda; Stiling, Peter; Hasenkampf, Clare; Hunter, Fiona; Bidochka, Michael; Riggs, Daniel (2010). "Chapter 5: Systems Biology of Cell Organization". Biology (Canadian ed.). United States of America: McGraw-Hill Ryerson. pp. 105–106. ISBN 978-0-07-074175-1.
- ↑ Burda, Patricie; Aebi, Markus (1999). "The dolichol pathway of N-linked glycosylation". Biochimica et Biophysica Acta (BBA) - General Subjects 1426 (2): 239. doi:10.1016/S0304-4165(98)00127-5.
- ↑ Imperiali, Barbara; o’Connor, Sarah E (1999). "Effect of N-linked glycosylation on glycopeptide and glycoprotein structure". Current Opinion in Chemical Biology 3 (6): 643. doi:10.1016/S1367-5931(99)00021-6. PMID 10600722.
- ↑ Patterson, Marc C. (2005). "Metabolic Mimics: The Disorders of N-Linked Glycosylation". Seminars in Pediatric Neurology 12 (3): 144–51. doi:10.1016/j.spen.2005.10.002. PMID 16584073.
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