Ameloblastoma

Ameloblastoma
Micrograph of an ameloblastoma showing the characteristic nuclear palisading and stellate reticulum. H&E stain.
Classification and external resources
ICD-10	D16.4-D16.5
ICD-9	213.0-213.1
ICD-O	9310/0
DiseasesDB	31676
MeSH	D000564

Ameloblastoma (from the early English word amel, meaning enamel + the Greek word blastos, meaning germ^[1]) is a rare, benign tumor of odontogenic epithelium (ameloblasts, or outside portion, of the teeth during development) much more commonly appearing in the lower jaw than the upper jaw.^[2] It was recognized in 1827 by Cusack.^[3] This type of odontogenic neoplasm was designated as an adamantinoma in 1885 by the French physician Louis-Charles Malassez.^[4] It was finally renamed to the modern name ameloblastoma in 1930 by Ivey and Churchill.^[5][6]

While these tumors are rarely malignant or metastatic (that is, they rarely spread to other parts of the body), and progress slowly, the resulting lesions can cause severe abnormalities of the face and jaw. Additionally, because abnormal cell growth easily infiltrates and destroys surrounding bony tissues, wide surgical excision is required to treat this disorder. If an aggressive tumor is left untreated, it can obstruct the nasal and oral airways making it impossible to breathe without oropharyngeal intervention.

Subtypes

There are three main clinical subtypes of ameloblastoma: unicystic, multicystic, peripheral.^[7] The peripheral subtype composes 2% of all ameloblastomas.^[2] Of all ameloblastomas in younger patients, unicystic ameloblastomas represent 6% of the cases.^[2] A fourth subtype, malignant, has been considered by some oncologic specialists, however, this form of the tumor is rare and may be simply a manifestation of one of the three main subtypes. Ameloblastoma also occurs in long bones, and another variant is Craniopharyngioma (Rathke's pouch tumour, Pituitary Ameloblastoma.)

Clinical features

A CT scan of a patient suffering from an ameloblastoma.

The resected left half of a mandible containing an ameloblastoma, initiated at the third molar

Ameloblastomas are often associated with the presence of unerupted teeth. Symptoms include painless swelling, facial deformity if severe enough, pain if the swelling impinges on other structures, loose teeth, ulcers, and periodontal (gum) disease. Lesions will occur in the mandible and maxilla,although 75% occur in the ascending ramus area and will result in extensive and grotesque deformitites of the mandible and maxilla. In the maxilla it can extend into the maxillary sinus and floor of the nose. The lesion has a tendency to expand the bony cortices because slow growth rate of the lesion allows time for periosteum to develop thin shell of bone ahead of the expanding lesion. This shell of bone cracks when palpated and this phenomenon is referred to as "Egg Shell Cracking" or crepitus, an important diagnostic feature. Ameloblastoma is tentatively diagnosed through radiographic examination and must be confirmed by histological examination (e.g., biopsy). Radiographically, it appears as a lucency in the bone of varying size and features—sometimes it is a single, well-demarcated lesion whereas it often demonstrates as a multiloculated "soap bubble" appearance. Resorption of roots of involved teeth can be seen in some cases, but is not unique to ameloblastoma. The disease is most often found in the posterior body and angle of the mandible, but can occur anywhere in either the maxilla or mandible.

Ameloblastoma is often associated with bony-impacted wisdom teeth—one of the many reasons some dentists recommend having them extracted.

Histopathology

Histopathology will show cells that have the tendency to move the nucleus away from the basement membrane. This process is referred to as "Reverse Polarization". The follicular type will have outer arrangement of columnar or palisaded ameloblast like cells and inner zone of triangular shaped cells resembling stellate reticulum in bell stage. The central cells sometimes degenerate to form central microcysts. The plexiform type has epithelium that proliferates in a "Fish Net Pattern". The plexiform ameloblastoma shows epithelium proliferating in a 'cord like fashion', hence the name 'plexiform'. There are layers of cells in between the proliferating epithelium with a well-formed desmosomal junctions, simulating spindle cell layers.

Ameloblastoma

Variants

The six different histopathological variants of ameloblastoma are desmoplastic, granular cell, basal cell, plexiform, follicular, and acanthomatous.^[8]

The acanthomatous variant is extremely rare.^[9]

One-third of ameloblastomas are plexiform, one-third are follicular. Other variants such as acanthomatous occur in older patients.^[2] In one center, desmoplastic ameloblastomas represented about 9% of all ameloblastomas encountered.^[10]

Treatment

Tracheal intubation is anticipated to be difficult in this child with a massive ameloblastoma.

While chemotherapy, radiation therapy, curettage and liquid nitrogen have been effective in some cases of ameloblastoma, surgical resection or enucleation remains the most definitive treatment for this condition. In a detailed study of 345 patients, chemotherapy and radiation therapy seemed to be contraindicated for the treatment of ameloblastomas.^[2] Thus, surgery is the most common treatment of this tumor. Because of the invasive nature of the growth, excision of normal tissue near the tumor margin is often required. Some have likened the disease to basal cell carcinoma (a skin cancer) in its tendency to spread to adjacent bony and sometimes soft tissues without metastasizing. While not a cancer that actually invades adjacent tissues, ameloblastoma is suspected to spread to adjacent areas of the jaw bone via marrow space. Thus, wide surgical margins that are clear of disease are required for a good prognosis. This is very much like surgical treatment of cancer. Often, treatment requires excision of entire portions of the jaw.

Radiation is ineffective^[2] in many cases of ameloblastoma.^[11] There have also been reports of sarcoma being induced as the result of using radiation to treat ameloblastoma.^[12] Chemotherapy is also often ineffective.^[12] However, there is some controversy regarding this^[13] and some indication that some ameloblastomas might be more responsive to radiation that previously thought.^[14][6]

While the Mayo Clinic recommends surgery for almost all ameloblastomas, there are situations in which a Mayo Clinic physician might recommend radiation therapy. These include malignancy, inability to completely remove the ameloblastoma, recurrence, unacceptable loss of function, and unacceptable cosmetic damage.^[15] In the case of radiotherapy, oncologists at the Mayo Clinic would use intensity-modulated radiotherapy.^[15]

Molecular biology

There is evidence that suppression of matrix metalloproteinase-2 may inhibit the local invasiveness of ameloblastoma, however, this was only demonstrated in vitro.^[16] There is also some research suggesting that α₅β₁ integrin may participate in the local invasiveness of ameloblastomas.^[17]

A recent study discovered a high frequency of BRAF V600E mutations (15 of 24 samples, 63%) in solid/multicystic ameloblastoma. These data suggests drugs targeting mutant BRAF as potential novel therapies for ameloblastoma.^[18]

Recurrence

Recurrence is common, although the recurrence rates for block resection followed by bone graft are lower than those of enucleation and curettage.^[19] Follicular variants appear to recur more than plexiform variants.^[2] Unicystic tumors recur less frequently than "non-unicystic" tumors.^[2] Persistent follow-up examination is essential for managing ameloblastoma.^[20] Follow up should occur at regular intervals for at least 10 years.^[21] Follow up is important, because 50% of all recurrences occur within 5 years postoperatively.^[2] Recurrence within a bone graft (following resection of the original tumor) does occur, but is less common.^[22] Seeding to the bone graft is suspected as a cause of recurrence.^[20] The recurrences in these cases seem to stem from the soft tissues, especially the adjacent periosteum.^[23] Recurrence has been reported to occur as many as 36 years after treatment.^[24]

To reduce the likelihood of recurrence within grafted bone, meticulous surgery^[22] with attention to the adjacent soft tissues is required.^[23][20]

Epidemiology

The annual incidence rates per million for ameloblastomas are 1.96, 1.20, 0.18 and 0.44 for black males, black females, white males and white females respectively.^[25] Ameloblastomas account for about one percent of all oral tumors^[12] and about 18% of odontogenic tumors.^[26] Men and women tend to be equally affected, although women tend to be 4 years younger than men when tumors first occur and tumors appear to be larger in females.^[2]

See also

• Ameloblastic fibroma
• Bone grafting
• Epithelial cell rests of Malassez
• List of cutaneous conditions
• Matrix Metalloproteinase-2
• Tooth development and Odontogenesis

References

External links

Wikimedia Commons has media related to Ameloblastoma.

• Radiology and Pathology MedPix Image Database