ARAF

A-Raf proto-oncogene, serine/threonine kinase

PDB rendering based on 1wxm.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
1WXM

Identifiers

Symbols

ARAF ; A-RAF; ARAF1; PKS2; RAFA1

External IDs

OMIM: 311010 MGI: 88065 HomoloGene: 1249 IUPHAR: 1933 ChEMBL: 1169596 GeneCards: ARAF Gene

EC number

2.7.11.1

Gene ontology
Molecular function	• protein kinase activity • protein serine/threonine kinase activity • receptor signaling protein activity • protein binding • ATP binding • metal ion binding
Cellular component	• cellular_component • mitochondrion
Biological process	• cellular protein modification process • protein phosphorylation • regulation of TOR signaling • regulation of proteasomal ubiquitin-dependent protein catabolic process • positive regulation of peptidyl-serine phosphorylation • intracellular signal transduction • negative regulation of apoptotic process
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr X:
47.42 – 47.43 Mb

Chr X:
20.8 – 20.86 Mb

PubMed search

Serine/threonine-protein kinase A-Raf or simply A-Raf is an enzyme that in humans is encoded by the ARAF gene.^[1] A-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases.^[2]

Interactions

ARAF has been shown to interact with:

References

↑ "Entrez Gene: ARAF V-raf murine sarcoma 3611 viral oncogene homolog".
↑ Mark GE, Seeley TW, Shows TB, Mountz JD (September 1986). "Pks, a raf-related sequence in humans". Proc. Natl. Acad. Sci. U.S.A. 83 (17): 6312–6. doi:10.1073/pnas.83.17.6312. PMC 386493. PMID 3529082.
↑ 3.0 3.1 3.2 3.3 3.4 Yuryev A, Wennogle LP (February 2003). "Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis". Genomics 81 (2): 112–25. doi:10.1016/S0888-7543(02)00008-3. PMID 12620389.
↑ Yin XL, Chen S, Yan J, Hu Y, Gu JX (February 2002). "Identification of interaction between MEK2 and A-Raf-1". Biochim. Biophys. Acta 1589 (1): 71–6. doi:10.1016/S0167-4889(01)00188-4. PMID 11909642.
↑ 5.0 5.1 5.2 Yuryev A, Ono M, Goff SA, Macaluso F, Wennogle LP (July 2000). "Isoform-Specific Localization of A-RAF in Mitochondria". Mol. Cell. Biol. 20 (13): 4870–8. doi:10.1128/MCB.20.13.4870-4878.2000. PMC 85938. PMID 10848612.
↑ Yin XL, Chen S, Gu JX (February 2002). "Identification of TH1 as an interaction partner of A-Raf kinase". Mol. Cell. Biochem. 231 (1–2): 69–74. doi:10.1023/A:1014437024129. PMID 11952167.

Belanger BF, Williams WJ, Yin TC (1976). "A flexible renewal process simulator for neural spike trains". IEEE Trans Biomed Eng 23 (3): 262–6. doi:10.1109/TBME.1976.324641. PMID 1262038.
Popescu NC, Mark GE (1989). "Localization of the pKs gene, a raf related sequence on human chromosomes X and 7". Oncogene 4 (4): 517–9. PMID 2717185.
Beck TW, Huleihel M, Gunnell M, Bonner TI, Rapp UR (1987). "The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus". Nucleic Acids Res. 15 (2): 595–609. doi:10.1093/nar/15.2.595. PMC 340454. PMID 3029685.
Papin C, Eychène A, Brunet A, Pagès G, Pouysségur J, Calothy G et al. (1995). "B-Raf protein isoforms interact with and phosphorylate Mek-1 on serine residues 218 and 222". Oncogene 10 (8): 1647–51. PMID 7731720.
Lee JE, Beck TW, Brennscheidt U, DeGennaro LJ, Rapp UR (1994). "The complete sequence and promoter activity of the human A-raf-1 gene (ARAF1)". Genomics 20 (1): 43–55. doi:10.1006/geno.1994.1125. PMID 8020955.
Alessi DR, Saito Y, Campbell DG, Cohen P, Sithanandam G, Rapp U et al. (1994). "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1". EMBO J. 13 (7): 1610–9. PMC 394991. PMID 8157000.
Gardner AM, Vaillancourt RR, Johnson GL (1993). "Activation of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase by G protein and tyrosine kinase oncoproteins". J. Biol. Chem. 268 (24): 17896–901. PMID 8394352.
Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
Wu X, Noh SJ, Zhou G, Dixon JE, Guan KL (1996). "Selective activation of MEK1 but not MEK2 by A-Raf from epidermal growth factor-stimulated Hela cells". J. Biol. Chem. 271 (6): 3265–71. doi:10.1074/jbc.271.6.3265. PMID 8621729.
Boldyreff B, Issinger OG (1997). "A-Raf kinase is a new interacting partner of protein kinase CK2 beta subunit". FEBS Lett. 403 (2): 197–9. doi:10.1016/S0014-5793(97)00010-0. PMID 9042965.
Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W et al. (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
Yuryev A, Ono M, Goff SA, Macaluso F, Wennogle LP (2000). "Isoform-specific localization of A-RAF in mitochondria". Mol. Cell. Biol. 20 (13): 4870–8. doi:10.1128/MCB.20.13.4870-4878.2000. PMC 85938. PMID 10848612.
King TR, Fang Y, Mahon ES, Anderson DH (2000). "Using a phage display library to identify basic residues in A-Raf required to mediate binding to the Src homology 2 domains of the p85 subunit of phosphatidylinositol 3'-kinase". J. Biol. Chem. 275 (46): 36450–6. doi:10.1074/jbc.M004720200. PMID 10967104.
Fang Y, Johnson LM, Mahon ES, Anderson DH (2002). "Two phosphorylation-independent sites on the p85 SH2 domains bind A-Raf kinase". Biochem. Biophys. Res. Commun. 290 (4): 1267–74. doi:10.1006/bbrc.2002.6347. PMID 11812000.
Yin XL, Chen S, Yan J, Hu Y, Gu JX (2002). "Identification of interaction between MEK2 and A-Raf-1". Biochim. Biophys. Acta 1589 (1): 71–6. doi:10.1016/S0167-4889(01)00188-4. PMID 11909642.
Yin XL, Chen S, Gu JX (2002). "Identification of TH1 as an interaction partner of A-Raf kinase". Mol. Cell. Biochem. 231 (1-2): 69–74. doi:10.1023/A:1014437024129. PMID 11952167.
Yuryev A, Wennogle LP (2003). "Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis". Genomics 81 (2): 112–25. doi:10.1016/S0888-7543(02)00008-3. PMID 12620389.
Liu W, Shen X, Yang Y, Yin X, Xie J, Yan J et al. (2004). "Trihydrophobin 1 is a new negative regulator of A-Raf kinase". J. Biol. Chem. 279 (11): 10167–75. doi:10.1074/jbc.M307994200. PMID 14684750.

PDB gallery

1wxm: Solution Structure of the N-terminal Ras-binding Domain (RBD) in Human a-Raf Kinase

ARAF

Interactions

References

Further reading