USP14

From Wikipedia, the free encyclopedia

Ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)

PDB rendering based on 1wgg.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
2AYN, 2AYO

Identifiers

Symbols

USP14; TGT

External IDs

OMIM: 607274 MGI: 1928898 HomoloGene: 3780 ChEMBL: 1293295 GeneCards: USP14 Gene

EC number

3.4.19.12

Gene Ontology
Molecular function	• cysteine-type endopeptidase activity • ubiquitin thiolesterase activity • ubiquitin-specific protease activity • endopeptidase inhibitor activity • protein binding • tRNA guanylyltransferase activity • proteasome binding
Cellular component	• proteasome complex • cytoplasm • plasma membrane • cell surface • cytoplasmic membrane-bounded vesicle • synapse
Biological process	• ubiquitin-dependent protein catabolic process • synaptic transmission • negative regulation of endopeptidase activity • regulation of chemotaxis • regulation of proteasomal protein catabolic process
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 18:
0.16 – 0.21 Mb

Chr 18:
10 – 10.05 Mb

PubMed search

Ubiquitin carboxyl-terminal hydrolase 14 is an enzyme that in humans is encoded by the USP14 gene.^[1]^[2]

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.^[2]

Interactions

USP14 has been shown to interact with CXCR4.^[3]

References

↑ Puente XS, Sanchez LM, Overall CM, Lopez-Otin C (Jul 2003). "Human and mouse proteases: a comparative genomic approach". Nat Rev Genet 4 (7): 544–58. doi:10.1038/nrg1111. PMID 12838346.
↑ 2.0 2.1 "Entrez Gene: USP14 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)".
↑ Mines, Marjelo A; Goodwin J Shawn, Limbird Lee E, Cui Fei-Fei, Fan Guo-Huang (Feb 2009). "Deubiquitination of CXCR4 by USP14 is critical for both CXCL12-induced CXCR4 degradation and chemotaxis but not ERK ativation". J. Biol. Chem. (United States) 284 (9): 5742–52. doi:10.1074/jbc.M808507200. ISSN 0021-9258. PMC 2645827. PMID 19106094. Cite uses deprecated parameters (help)

Wilson, S.M. "et al." (2002). "Synaptic defects in ataxia mice result from a mutation in Usp14, encoding a ubiquitin-specific protease.". Nature Genetics 33 (32): 420–425. doi:10.1038/ng1006. PMID 12368914.
D'Andrea A, Pellman D (1999). "Deubiquitinating enzymes: a new class of biological regulators.". Crit. Rev. Biochem. Mol. Biol. 33 (5): 337–52. doi:10.1080/10409239891204251. PMID 9827704.
Deshpande KL, Seubert PH, Tillman DM, et al. (1996). "Cloning and characterization of cDNA encoding the rabbit tRNA-guanine transglycosylase 60-kilodalton subunit.". Arch. Biochem. Biophys. 326 (1): 1–7. doi:10.1006/abbi.1996.0039. PMID 8579355.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Reuter TY, Medhurst AL, Waisfisz Q, et al. (2003). "Yeast two-hybrid screens imply involvement of Fanconi anemia proteins in transcription regulation, cell signaling, oxidative metabolism, and cellular transport.". Exp. Cell Res. 289 (2): 211–21. doi:10.1016/S0014-4827(03)00261-1. PMID 14499622.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Dennehey BK, Gutches DG, McConkey EH, Krauter KS (2004). "Inversion, duplication, and changes in gene context are associated with human chromosome 18 evolution.". Genomics 83 (3): 493–501. doi:10.1016/j.ygeno.2003.08.017. PMID 14962675.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Hu M, Li P, Song L, et al. (2005). "Structure and mechanisms of the proteasome-associated deubiquitinating enzyme USP14.". EMBO J. 24 (21): 3747–56. doi:10.1038/sj.emboj.7600832. PMC 1276716. PMID 16211010.
Shinji S, Naito Z, Ishiwata S, et al. (2006). "Ubiquitin-specific protease 14 expression in colorectal cancer is associated with liver and lymph node metastases.". Oncol. Rep. 15 (3): 539–43. PMID 16465409.
Crimmins S, Jin Y, Wheeler C, et al. (2006). "Transgenic rescue of ataxia mice with neuronal-specific expression of ubiquitin-specific protease 14.". J. Neurosci. 26 (44): 11423–31. doi:10.1523/JNEUROSCI.3600-06.2006. PMID 17079671.

PDB gallery

1wgg: Solution Structure of the N-terminal Ubiquitin-like Domain of Mouse Ubiquitin Specific Protease 14 (USP14)

2ayn: Structure of USP14, a proteasome-associated deubiquitinating enzyme

2ayo: Structure of USP14 bound to ubquitin aldehyde

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USP14

Interactions

References

Further reading